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Literature summary for 1.1.1.118 extracted from

  • Giardina, P.; DeBasia, M.G.; DeRosa, M.; Gambacort, A.; Buonocore, V.
    Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus (1986), Biochem. J., 239, 517-522.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol rapid inactivation at 1 mM Saccharolobus solfataricus
5,5'-dithiobis-(2-nitrobenzoic acid)
-
Saccharolobus solfataricus
EDTA 5% residual activity at 50 mM, addition of Mg2+ in excess restored the initial activity Saccharolobus solfataricus
Guanidinium chloride rapid inactivation at 4 M Saccharolobus solfataricus
additional information D-alactose, D-mannose and D-ribose, in concentrations as high as 0.04 mM do not inhibit glucose oxidation by NAD+ Saccharolobus solfataricus
N-ethylmaleimide
-
Saccharolobus solfataricus
NADPH inhibits the NAD+-dependent carbohydrate oxidations in a competitive manner with respect to NAD+ Saccharolobus solfataricus
tetrahydrofuran
-
Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
NADP+ in the presence of D-galactose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
0.03
-
NADP+ in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
0.03
-
NADP+ in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
0.44
-
D-glucose in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.2
-
NAD+ in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.2
-
NAD+ in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
2.2
-
D-xylose in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
8
-
D-glucose in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
22
-
D-galactose in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
68
-
D-xylose in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximally active at 20 mM Saccharolobus solfataricus
Mg2+ maximally active at 20 mM Saccharolobus solfataricus
Mn2+ maximally active at 20 mM Saccharolobus solfataricus
additional information Zn2+ is partially effective, whereas Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30100
-
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS Saccharolobus solfataricus
60200
-
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride Saccharolobus solfataricus
124000
-
gel filtration Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone stable in the presence of chaotropic agents and water-miscible organic solvents such as acetone Saccharolobus solfataricus
Ethanol a slow time-dependent decrease of activity is observed in the presence of ethanol Saccharolobus solfataricus
Methanol stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol Saccharolobus solfataricus
additional information 2 M urea and 0.05% (w/v) SDS do not cause any loss of enzymic activity Saccharolobus solfataricus
SDS a slow time-dependent decrease of activity is observed in the presence of 0.1% SDS Saccharolobus solfataricus
urea a slow time-dependent decrease of activity is observed in the presence of 4 M urea Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
SP-Sephadex C-50 gel filtration, phenyl-Sepharose column chromatography, and Affi-Gel Blue matrix gel filtration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
unpurified enzyme, at 70°C Saccharolobus solfataricus
437
-
after 623fold purification, at 70°C Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
37°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 40 days, 50% loss of activity Saccharolobus solfataricus
4°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, several months, remains stable Saccharolobus solfataricus
70°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 45 h, 50% loss of activity Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2-deoxy-D-glucose + NAD+ 26% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADH
-
?
2-amino-2-deoxy-D-glucose + NADP+ 5% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
2-deoxy-D-glucose + NAD+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
?
2-deoxy-D-glucose + NAD+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus MT-4 / DSM 5833 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
?
2-deoxy-D-glucose + NADP+ 25% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
6-deoxy-D-glucose + NAD+ 66% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus 6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
?
6-deoxy-D-glucose + NAD+ 66% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus MT-4 / DSM 5833 6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
?
6-deoxy-D-glucose + NADP+ 9% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
D-altrose + NAD+ 5% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus D-altrono-1,5-lactone + NADH + H+
-
?
D-altrose + NADP+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
D-galactose + NAD+ 15% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus D-galactono-1,5-lactone + NADH + H+
-
?
D-glucose + NAD+ 100% activity Saccharolobus solfataricus D-glucono-1,5-lactone + NADH + H+
-
?
D-glucose + NAD+ 100% activity Saccharolobus solfataricus MT-4 / DSM 5833 D-glucono-1,5-lactone + NADH + H+
-
?
D-glucose + NADP+ 9% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH + H+
-
?
D-glucose + NADP+ 9% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus MT-4 / DSM 5833 D-glucono-1,5-lactone + NADPH + H+
-
?
D-gulose + NAD+ 8% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADH
-
?
D-gulose + NADP+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
D-idose + NAD+ 65% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADH
-
?
D-idose + NADP+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
D-mannose + NADP+ 4% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
D-ribose + NADP+ 4% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
D-xylose + NAD+ 26% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADH
-
?
D-xylose + NADP+ 28% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
?
additional information no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+ Saccharolobus solfataricus ?
-
?
additional information no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+ Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride Saccharolobus solfataricus
homotetramer 4 * 30100, gel filtration, in the presence of 5% (w/v) SDS Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
glucose dehydrogenase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
77
-
-
Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 the enzyme stability does not vary significantly in the pH range 5-9 Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharolobus solfataricus
NADP+
-
Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.075
-
NADPH using D-glucose as a substrate Saccharolobus solfataricus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1
-
at 25°C and pH 9 Saccharolobus solfataricus N-ethylmaleimide
3
-
at 25°C and pH 9 Saccharolobus solfataricus 5,5'-dithiobis-(2-nitrobenzoic acid)