Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | rapid inactivation at 1 mM | Saccharolobus solfataricus | |
5,5'-dithiobis-(2-nitrobenzoic acid) | - |
Saccharolobus solfataricus | |
EDTA | 5% residual activity at 50 mM, addition of Mg2+ in excess restored the initial activity | Saccharolobus solfataricus | |
Guanidinium chloride | rapid inactivation at 4 M | Saccharolobus solfataricus | |
additional information | D-alactose, D-mannose and D-ribose, in concentrations as high as 0.04 mM do not inhibit glucose oxidation by NAD+ | Saccharolobus solfataricus | |
N-ethylmaleimide | - |
Saccharolobus solfataricus | |
NADPH | inhibits the NAD+-dependent carbohydrate oxidations in a competitive manner with respect to NAD+ | Saccharolobus solfataricus | |
tetrahydrofuran | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADP+ | in the presence of D-galactose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
0.03 | - |
NADP+ | in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
0.03 | - |
NADP+ | in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
0.44 | - |
D-glucose | in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
1.2 | - |
NAD+ | in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
1.2 | - |
NAD+ | in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
2.2 | - |
D-xylose | in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
8 | - |
D-glucose | in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
22 | - |
D-galactose | in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus | |
68 | - |
D-xylose | in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | maximally active at 20 mM | Saccharolobus solfataricus | |
Mg2+ | maximally active at 20 mM | Saccharolobus solfataricus | |
Mn2+ | maximally active at 20 mM | Saccharolobus solfataricus | |
additional information | Zn2+ is partially effective, whereas Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30100 | - |
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS | Saccharolobus solfataricus |
60200 | - |
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride | Saccharolobus solfataricus |
124000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | stable in the presence of chaotropic agents and water-miscible organic solvents such as acetone | Saccharolobus solfataricus |
Ethanol | a slow time-dependent decrease of activity is observed in the presence of ethanol | Saccharolobus solfataricus |
Methanol | stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol | Saccharolobus solfataricus |
additional information | 2 M urea and 0.05% (w/v) SDS do not cause any loss of enzymic activity | Saccharolobus solfataricus |
SDS | a slow time-dependent decrease of activity is observed in the presence of 0.1% SDS | Saccharolobus solfataricus |
urea | a slow time-dependent decrease of activity is observed in the presence of 4 M urea | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
SP-Sephadex C-50 gel filtration, phenyl-Sepharose column chromatography, and Affi-Gel Blue matrix gel filtration | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
unpurified enzyme, at 70°C | Saccharolobus solfataricus |
437 | - |
after 623fold purification, at 70°C | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
37°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 40 days, 50% loss of activity | Saccharolobus solfataricus |
4°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, several months, remains stable | Saccharolobus solfataricus |
70°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 45 h, 50% loss of activity | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-2-deoxy-D-glucose + NAD+ | 26% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADH | - |
? | |
2-amino-2-deoxy-D-glucose + NADP+ | 5% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
2-deoxy-D-glucose + NAD+ | 12% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
2-deoxy-D-glucose + NAD+ | 12% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus MT-4 / DSM 5833 | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
2-deoxy-D-glucose + NADP+ | 25% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
6-deoxy-D-glucose + NAD+ | 66% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | 6-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
6-deoxy-D-glucose + NAD+ | 66% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus MT-4 / DSM 5833 | 6-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
6-deoxy-D-glucose + NADP+ | 9% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
D-altrose + NAD+ | 5% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | D-altrono-1,5-lactone + NADH + H+ | - |
? | |
D-altrose + NADP+ | 12% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
D-galactose + NAD+ | 15% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | D-galactono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | 100% activity | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | 100% activity | Saccharolobus solfataricus MT-4 / DSM 5833 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NADP+ | 9% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose + NADP+ | 9% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus MT-4 / DSM 5833 | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-gulose + NAD+ | 8% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADH | - |
? | |
D-gulose + NADP+ | 12% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
D-idose + NAD+ | 65% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADH | - |
? | |
D-idose + NADP+ | 12% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
D-mannose + NADP+ | 4% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
D-ribose + NADP+ | 4% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
D-xylose + NAD+ | 26% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADH | - |
? | |
D-xylose + NADP+ | 28% activity compared to D-glucose in combination with NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
additional information | no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+ | Saccharolobus solfataricus | ? | - |
? | |
additional information | no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+ | Saccharolobus solfataricus MT-4 / DSM 5833 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride | Saccharolobus solfataricus |
homotetramer | 4 * 30100, gel filtration, in the presence of 5% (w/v) SDS | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
glucose dehydrogenase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
77 | - |
- |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | the enzyme stability does not vary significantly in the pH range 5-9 | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharolobus solfataricus | |
NADP+ | - |
Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
NADPH | using D-glucose as a substrate | Saccharolobus solfataricus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1 | - |
at 25°C and pH 9 | Saccharolobus solfataricus | N-ethylmaleimide | |
3 | - |
at 25°C and pH 9 | Saccharolobus solfataricus | 5,5'-dithiobis-(2-nitrobenzoic acid) |