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Literature summary for 1.1.1.11 extracted from

  • Neuberger, M.S.; Patterson, R.A.; Hartley, B.S.
    Purification and properties of Klebsiella aerogenes D-arabitol dehydrogenase (1979), Biochem. J., 183, 31-42.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene over-expressed in Escherichia coli K12 Klebsiella aerogenes

General Stability

General Stability Organism
glycerol stabilizes Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
NAD+
-
Klebsiella aerogenes
20
-
D-arabinitol
-
Klebsiella aerogenes
70
-
D-mannitol
-
Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
gel filtration, ultracentrifugation Klebsiella aerogenes
44000
-
sedimentation equilibrium Klebsiella aerogenes
46000
-
calculated from amino acid composition Klebsiella aerogenes
46500
-
1 * 46500, SDS-PAGE Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-arabinitol + NAD+ Klebsiella aerogenes
-
D-xylulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
i.e. Klebsiella pneumoniae, gene expression in Escherichia coli K12
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.1
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
-20°C, protein concentration 50 mg/ml, 50% v/v glycerol, stable 3 months, loss of activity less than 50% Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinitol + NAD+
-
Klebsiella aerogenes D-xylulose + NADH + H+
-
?
D-mannitol + NAD+
-
Klebsiella aerogenes D-fructose + NADH + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 46500, SDS-PAGE Klebsiella aerogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
enzyme assay at Klebsiella aerogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
20 min Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Klebsiella aerogenes