Cloned (Comment) | Organism |
---|---|
gene over-expressed in Escherichia coli K12 | Klebsiella aerogenes |
General Stability | Organism |
---|---|
glycerol stabilizes | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NAD+ | - |
Klebsiella aerogenes | |
20 | - |
D-arabinitol | - |
Klebsiella aerogenes | |
70 | - |
D-mannitol | - |
Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration, ultracentrifugation | Klebsiella aerogenes |
44000 | - |
sedimentation equilibrium | Klebsiella aerogenes |
46000 | - |
calculated from amino acid composition | Klebsiella aerogenes |
46500 | - |
1 * 46500, SDS-PAGE | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinitol + NAD+ | Klebsiella aerogenes | - |
D-xylulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
i.e. Klebsiella pneumoniae, gene expression in Escherichia coli K12 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein from Escherichia coli | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.1 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
-20°C, protein concentration 50 mg/ml, 50% v/v glycerol, stable 3 months, loss of activity less than 50% | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinitol + NAD+ | - |
Klebsiella aerogenes | D-xylulose + NADH + H+ | - |
? | |
D-mannitol + NAD+ | - |
Klebsiella aerogenes | D-fructose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46500, SDS-PAGE | Klebsiella aerogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
enzyme assay at | Klebsiella aerogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella aerogenes |