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Literature summary for 1.1.1.108 extracted from
- Purification and properties of carnitine dehydrogenase from Pseudomonas putida (1988), Biochim. Biophys. Acta, 957, 335-339.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
NADH | potassium phosphate buffer, pH 7 | Pseudomonas putida |  |
| 0.067 | - |
NADH | Tris-HCl buffer, pH 9 | Pseudomonas putida | |
| 0.2 | - |
NAD+ | - |
Pseudomonas putida | |
| 1.1 | - |
3-dehydrocarnitine | potassium phosphate buffer, pH 7 | Pseudomonas putida | |
| 1.54 | - |
3-dehydrocarnitine | Tris-HCl buffer, pH 9 | Pseudomonas putida | |
| 6.25 | - |
L-carnitine | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Pseudomonas putida |
| 60000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxy-4-trimethylaminobutyrate + NAD+ | Pseudomonas putida | - |
3-dehydrocarnitine + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephadex G-200, 5'-AMP-Sepharose 4B, gel filtration | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 55.3 | - |
- |
Pseudomonas putida |
| 240 | - |
reduction of 3-dehydrocarnitine at pH 7-7.5 | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxy-4-trimethylaminobutyrate + NAD+ | - |
Pseudomonas putida | 3-dehydrocarnitine + NADH + H+ | - |
r | |
| carnitine + NAD+ | - |
Pseudomonas putida | 3-dehydrocarnitine + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity declines rapidly above 35°C | Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 30 | - |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | reduction of 3-dehydrocarnitine | Pseudomonas putida |
| 9 | - |
oxidation of L-carnitine | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas putida | |
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