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Literature summary for 1.1.1.107 extracted from

  • Yokochi, N.; Yoshikane, Y.; Trongpanich, Y.; Ohnishi, K.; Yagi, T.
    Molecular cloning, expression, and properties of an unusual aldo-keto reductase family enzyme, pyridoxal 4-dehydrogenase, that catalyzes irreversible oxidation of pyridoxal (2004), J. Biol. Chem., 279, 37377-37384.
    View publication on PubMed
Search for more literature for 1.1.1.107

Cloned(Commentary)

Cloned (Comment) Organism
-
 Microbacterium luteolum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 NAD+ cosubstrate L-fucose, pH 8.0, 30°C Microbacterium luteolum
0.05
-
 NAD+ cosubstrate pyridoxal, pH 8.0, 30°C Microbacterium luteolum
0.41
-
 L-fucose pH 8.0, 30°C Microbacterium luteolum
0.58
-
 NADP+ cosubstrate L-fucose, pH 8.0, 30°C Microbacterium luteolum
0.91
-
 D-arabinose pH 8.0, 30°C Microbacterium luteolum
3.5
-
 L-Xylose pH 8.0, 30°C Microbacterium luteolum
13
-
 pyridoxal pH 8.0, 30°C Microbacterium luteolum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Microbacterium luteolum
84000
-
 gel filtration Microbacterium luteolum

Organism

Organism UniProt Comment Textmining
Microbacterium luteolum Q76KC2
-
-
Microbacterium luteolum YK-1 Q76KC2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
635
-
 pH 8.0, 30°C Microbacterium luteolum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose + NAD+
-
 Microbacterium luteolum ? + NADH
-
 ?
D-arabinose + NAD+
-
 Microbacterium luteolum YK-1 ? + NADH
-
 ?
D-arabinose + NAD+
-
 Microbacterium luteolum D-arabinono-1,4-lactone + NADH + H+
-
 ?
L-fucose + NAD+
-
 Microbacterium luteolum ? + NADH
-
 ?
L-fucose + NAD+
-
 Microbacterium luteolum YK-1 ? + NADH
-
 ?
L-fucose + NAD+
-
 Microbacterium luteolum L-fucono-1,5-lactone + NADH + H+
-
 ?
L-xylose + NAD+
-
 Microbacterium luteolum ? + NADH
-
 ?
L-xylose + NAD+
-
 Microbacterium luteolum YK-1 ? + NADH
-
 ?
pyridoxal + NAD+
-
 Microbacterium luteolum 4-pyridoxolactone + NADH + H+
-
 ?
pyridoxal + NAD+
-
 Microbacterium luteolum YK-1 4-pyridoxolactone + NADH + H+
-
 ?
pyridoxal + NADP+
-
 Microbacterium luteolum 4-pyridoxolactone + NADPH + H+
-
 ?
pyridoxal + NADP+
-
 Microbacterium luteolum YK-1 4-pyridoxolactone + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Microbacterium luteolum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Microbacterium luteolum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Microbacterium luteolum
45
-
 10 min, 75% inactivation Microbacterium luteolum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
59.2
-
 pyridoxal pH 8.0, 30°C Microbacterium luteolum
125
-
 L-fucose pH 8.0, 30°C Microbacterium luteolum
175
-
 D-arabinose pH 8.0, 30°C Microbacterium luteolum
214
-
 L-Xylose pH 8.0, 30°C Microbacterium luteolum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 substrate pyridoxal Microbacterium luteolum
9.5
-
 substrate L-fucose Microbacterium luteolum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Microbacterium luteolum
NADP+
-
 Microbacterium luteolum