KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
D-pantoate | at pH 10.0 | Pseudomonas sp. P2 | |
0.5 | - |
ketopantoate | - |
Pseudomonas sp. P2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pantoate + NAD+ | Pseudomonas sp. P2 | - |
(R)-4-dehydropantoate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. P2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
50°C, protamine sulfate, calcium phosphate gel, ammonium sulfate, 50°C | Pseudomonas sp. P2 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas sp. P2 |
Storage Stability | Organism |
---|---|
-20°C, 2 mM potassium phosphate, pH 7.0, 0.01% EDTA, 1 mM mercaptoethanol, several months, no loss of activity | Pseudomonas sp. P2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pantoate + NAD+ | - |
Pseudomonas sp. P2 | (R)-4-dehydropantoate + NADH | - |
r | |
2-oxo-pantoate + NAD+ | - |
Pseudomonas sp. P2 | 2-oxo-4-dehydropantoate | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | linear activity increase | Pseudomonas sp. P2 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. P2 |