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Literature summary for 1.1.1.106 extracted from

  • Mäntsälä, P.
    Purification of pantoate and dimethylmalate dehydrogenase from Pseudomonas fluorescens UK-1 (1978), Biochim. Biophys. Acta, 526, 25-33.
    View publication on PubMed
Search for more literature for 1.1.1.106

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 4 * 24000, SDS-PAGE Pseudomonas fluorescens
80000
-
 ultracentrifugation Pseudomonas fluorescens
86000
-
 gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-pantoate + NAD+ Pseudomonas fluorescens
-
 (R)-4-dehydropantoate + NADH
-
 ?
(R)-pantoate + NAD+ Pseudomonas fluorescens UK1
-
 (R)-4-dehydropantoate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
 UK1
-
Pseudomonas fluorescens UK1
-
 UK1
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, 50°C, gel filtration, hydroxyapatite, 60°C, gel filtration Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.34
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pantoate + NAD+
-
 Pseudomonas fluorescens (R)-4-dehydropantoate + NADH
-
 ?
(R)-pantoate + NAD+
-
 Pseudomonas fluorescens UK1 (R)-4-dehydropantoate + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 24000, SDS-PAGE Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas fluorescens