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Literature summary for 1.1.1.103 extracted from

  • Desjardins, M.; Mak, W.S.; OBrien, T.E.; Carlin, D.A.; Tantillo, D.J.; Siegel, J.B.
    Systematic functional analysis of active-site residues in l-threonine dehydrogenase from Thermoplasma volcanium (2017), ACS Omega, 2, 3308-3314 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.103

Protein Variants

Protein Variants Comment Organism
D180A 228fold decrease in the catalytic efficiency Thermoplasma volcanium
G114A mutation cause a 4fold increase in catalytic efficiency Thermoplasma volcanium
I113A 6fold decrease in the catalytic efficiency Thermoplasma volcanium
S74A 1886fold decrease in the catalytic efficiency Thermoplasma volcanium
T178A 18fold decrease in the catalytic efficiency Thermoplasma volcanium
T179A 60fold decrease in the catalytic efficiency Thermoplasma volcanium
W273a 413fold decrease in the catalytic efficiency Thermoplasma volcanium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00057
-
 L-threonine 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium
0.0026
-
 L-threonine 25°C, pH 10, wild-type enzyme Thermoplasma volcanium
0.0088
-
 L-threonine 25°C, pH 10, mutant enzyme I113A Thermoplasma volcanium
0.011
-
 L-threonine 25°C, pH 10, mutant enzyme T178A Thermoplasma volcanium
0.043
-
 L-threonine 25°C, pH 10, mutant enzyme D180A Thermoplasma volcanium
0.061
-
 L-serine 25°C, pH 10, wild-type enzyme Thermoplasma volcanium
0.18
-
 L-serine 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium
0.36
-
 (R)-(-)-1-amino-2-propanol 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium
1.6
-
 L-serine 25°C, pH 10, mutant enzyme T178A Thermoplasma volcanium
1.8
-
 L-serine 25°C, pH 10, mutant enzyme I113A Thermoplasma volcanium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Thermoplasma volcanium
-
 L-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+ Thermoplasma volcanium ATCC 51530
-
 L-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma volcanium Q97BK3
-
-
Thermoplasma volcanium ATCC 51530 Q97BK3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(-)-1-amino-2-propanol + NAD+ activity with mutant enzyme G114A. No activity with wild-type enzyme Thermoplasma volcanium ?
-
 ?
(R)-(-)-1-amino-2-propanol + NAD+ activity with mutant enzyme G114A. No activity with wild-type enzyme Thermoplasma volcanium ATCC 51530 ?
-
 ?
L-serine + NAD+
-
 Thermoplasma volcanium L-2-amino-3-oxopropionate + NADH + H+
-
 ?
L-serine + NAD+
-
 Thermoplasma volcanium ATCC 51530 L-2-amino-3-oxopropionate + NADH + H+
-
 ?
L-threonine + NAD+
-
 Thermoplasma volcanium L-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+
-
 Thermoplasma volcanium ATCC 51530 L-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threoninol + NAD+
-
 Thermoplasma volcanium ?
-
 ?
L-threoninol + NAD+
-
 Thermoplasma volcanium ATCC 51530 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0085
-
 (R)-(-)-1-amino-2-propanol 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium
0.01
-
 L-serine 25°C, pH 10, wild-type enzyme Thermoplasma volcanium
0.013
-
 L-serine 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium
0.014
-
 L-serine 25°C, pH 10, mutant enzyme T178A Thermoplasma volcanium
0.026
-
 L-serine 25°C, pH 10, mutant enzyme I113A Thermoplasma volcanium
0.37
-
 L-threonine 25°C, pH 10, mutant enzyme D180A Thermoplasma volcanium
0.73
-
 L-threonine 25°C, pH 10, mutant enzyme I113A Thermoplasma volcanium
1.6
-
 L-threonine 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium
1.7
-
 L-threonine 25°C, pH 10, wild-type enzyme Thermoplasma volcanium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00000087
-
 L-threoninol 25°C, pH 10, wild-type enzyme Thermoplasma volcanium
0.0000035
-
 (R)-(-)-1-amino-2-propanol 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium
0.0000095
-
 L-serine 25°C, pH 10, mutant enzyme T178A Thermoplasma volcanium
0.000023
-
 L-threoninol 25°C, pH 10, wild-type enzyme Thermoplasma volcanium
0.000055
-
 L-serine 25°C, pH 10, mutant enzyme I113A Thermoplasma volcanium
0.00017
-
 L-serine 25°C, pH 10, wild-type enzyme Thermoplasma volcanium
0.00035
-
 L-threonine 25°C, pH 10, mutant enzyme T178A Thermoplasma volcanium
0.00035
-
 L-threonine 25°C, pH 10, mutant enzyme W273A Thermoplasma volcanium
0.00054
-
 L-serine 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium
0.0016
-
 L-threonine 25°C, pH 10, mutant enzyme T179A Thermoplasma volcanium
0.0029
-
 L-threonine 25°C, pH 10, mutant enzyme S74A Thermoplasma volcanium
0.011
-
 L-threonine 25°C, pH 10, mutant enzyme D180A Thermoplasma volcanium
0.11
-
 L-threonine 25°C, pH 10, mutant enzyme I113A Thermoplasma volcanium
0.66
-
 L-threonine 25°C, pH 10, wild-type enzyme Thermoplasma volcanium
2.7
-
 L-threonine 25°C, pH 10, mutant enzyme G114A Thermoplasma volcanium