Literature summary for 1.1.1.103 extracted from

Bowyer, A.; Mikolajek, H.; Wright, J.N.; Coker, A.; Erskine, P.T.; Cooper, J.B.; Bashir, Q.; Rashid, N.; Jamil, F.; Akhtar, M.
Crystallization and preliminary X-ray diffraction analysis of L-threonine dehydrogenase (TDH) from the hyperthermophilic archaeon Thermococcus kodakaraensis (2008), Acta Crystallogr. Sect. F, 64, 828-830.

Search for more literature for 1.1.1.103

Cloned(Commentary)

Cloned (Comment)	Organism
ligated into the pTZ57R/T cloning vector. The recombinant pTZ-tdh plasmid digested with NcoI and EcoRI and the TDH gene ligated with pET-8c, giving the recombinant pET-tdh plasmid, used to transform Escherichia coli DH5alpha competent cells. Recombinant and purified pET-tdh plasmid expressed in Escherichia coli BL21 (DE3)	Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
by hanging-drop vapour-diffusion method, to 2.6 A resolution. Crystals grow in the tetragonal space group P43212, with unit-cell parameters a=b=124.5, c=271.1 A. There are four molecules in the asymmetric unit of the crystal	Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38016	-	4 * 38016, electrospray mass spectrometry and gel filtration	Thermococcus kodakarensis

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	Q5JI69	-	-

Purification (Commentary)

Purification (Comment)	Organism
by sonication, centrifugation, heating of the soluble fraction, anion-exchange and hydrophobic interaction chromatography	Thermococcus kodakarensis

Storage Stability

Storage Stability	Organism
50 mM TrisHCl buffer, pH 8.0	Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-threonine + NAD+	-	Thermococcus kodakarensis	(2S)-2-amino-3-oxobutanoate + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 38016, electrospray mass spectrometry and gel filtration	Thermococcus kodakarensis

Synonyms

Synonyms	Comment	Organism
L-threonine dehydrogenase	-	Thermococcus kodakarensis
TDH	-	Thermococcus kodakarensis

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Thermococcus kodakarensis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)