Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1.26 mM, 41% inhibition after 1 h, 82% inhibition after 2 h, no change in remaining activity after removal of 1,10-phenanthroline | Escherichia coli K-12 | |
dipicolinic acid | 40 mM, 99% inhibition after 1 h, complete loss of enzyme-bound Zn2+ | Escherichia coli K-12 | |
EDTA | 50 mM, 99% inhibition after 1 h, complete loss of enzyme-bound Zn2+ | Escherichia coli K-12 | |
iodoacetate | iodoacetate reacts with 1 sulfhydryl group per subunit of the enzyme, enzyme retains 15% of its initial activity | Escherichia coli K-12 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | enzyme contains 1 Zn2+ atom per subunit | Escherichia coli K-12 | |
Zn2+ | exchange of Zn2+ with Co2+ or Cd2+ does not change the specific activity of the enzyme | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Escherichia coli K-12 | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Escherichia coli K-12 | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli K-12 |