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Literature summary for 1.1.1.102 extracted from

  • Gupta, S.D.; Gable, K.; Han, G.; Borovitskaya, A.; Selby, L.; Dunn, T.M.; Harmon, J.M.
    Tsc10p and FVT1: Topologically distinct short-chain reductases required for long-chain base synthesis in yeast and mammals (2009), J. Lipid Res., 50, 1630-1640.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
FVT1 expressed in CHO cells or yeast Homo sapiens
TSC10p expressed in CHO cells or yeast Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A175T mutation is deleterious to the human enzyme Homo sapiens
additional information mutation of any of the three residues that constitute the catalytic triad of FVT1 do not completely inactivate the enzyme, as it is able to support growth of tsc10DELTA mutant cells at 26°C, but not at 37°C. Deletion of residues 4-26 from the FVT1-GFP protein abolishes ER localization Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
siRNA reduces expression of transfected FVT1 mRNA and protein by at least 70% Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm C-terminus of FVT1 Homo sapiens 5737
-
cytoplasm N- and C-terminus of Tsc10p Saccharomyces cerevisiae 5737
-
endoplasmic reticulum N-terminal membrane-spanning domain in FVT1 orients to place it in the endoplasmic reticulum lumen Homo sapiens 5783
-
endoplasmic reticulum Tsc10p localizes to the yeast endoplasmic reticulum membrane Saccharomyces cerevisiae 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
gel filtration Saccharomyces cerevisiae
150000
-
gel filtration1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydrosphinganine + NADPH + H+
-
Homo sapiens sphinganine + NADP+
-
?
3-dehydrosphinganine + NADPH + H+
-
Saccharomyces cerevisiae sphinganine + NADP+
-
?
additional information FVT1 can replace Tsc10p in yeast Homo sapiens ?
-
?
additional information FVT1 is able to support growth of tsc10DELTA mutant cells at 26°C, but not at 37°C Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
tetramer gel filtration Homo sapiens
tetramer gel filtration Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Fvt1
-
Homo sapiens
Tsc10p
-
Saccharomyces cerevisiae