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Literature summary for 1.1.1.101 extracted from

  • Racenis, P.V.; Lai, J.L.; Das, A.K.; Mullick, P.C.; Hajra, A.K.; Greenberg, M.L.
    The acyl dihydroxyacetone phosphate pathway enzymes for glycerolipid biosynthesis are present in the yeast Saccharomyces cerevisiae (1992), J. Bacteriol., 174, 5704-5710.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
divalent cations i.e. Cu2+, Co2+, Ca2+, Mg2+, Mn2+, Ni2+, Zn2+, strong inhibition Saccharomyces cerevisiae
EDTA weak inhibition Saccharomyces cerevisiae
F- weak inhibition Saccharomyces cerevisiae
N-ethylmaleimide strong inhibitor Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
NADPH cosubstrate hexadecyldihydroxyacetone phosphate Saccharomyces cerevisiae
0.02
-
NADPH
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyldihydroxyacetone phosphate + NADPH Saccharomyces cerevisiae acyldihydroxyacetone phosphate pathway 1-acyl-sn-glycerol 3-phosphate + NADP+
-
?
acyldihydroxyacetone phosphate + NADPH Saccharomyces cerevisiae reaction in non-ether lipids biosynthesis 1-acyl-sn-glycerol 3-phosphate + NADP+
-
?
alkyldihydroxyacetone phosphate + NADPH + H+ Saccharomyces cerevisiae acyldihydroxyacetone phosphate pathway 1-alkyl-sn-glycerol 3-phosphate + NADP+
-
?
alkyldihydroxyacetone phosphate + NADPH + H+ Saccharomyces cerevisiae reaction in ether lipids biosynthesis 1-alkyl-sn-glycerol 3-phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
wild-type strain
-

Storage Stability

Storage Stability Organism
-20┬░C, membrane fraction stable Saccharomyces cerevisiae
-80┬░C, membrane fraction stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyldihydroxyacetone phosphate + NADPH
-
Saccharomyces cerevisiae 1-acyl-sn-glycerol 3-phosphate + NADP+
-
?
acyldihydroxyacetone phosphate + NADPH acyldihydroxyacetone phosphate pathway Saccharomyces cerevisiae 1-acyl-sn-glycerol 3-phosphate + NADP+
-
?
acyldihydroxyacetone phosphate + NADPH reaction in non-ether lipids biosynthesis Saccharomyces cerevisiae 1-acyl-sn-glycerol 3-phosphate + NADP+
-
?
alkyldihydroxyacetone phosphate + NADPH + H+
-
Saccharomyces cerevisiae 1-alkyl-sn-glycerol 3-phosphate + NADP+
-
?
alkyldihydroxyacetone phosphate + NADPH + H+ acyldihydroxyacetone phosphate pathway Saccharomyces cerevisiae 1-alkyl-sn-glycerol 3-phosphate + NADP+
-
?
alkyldihydroxyacetone phosphate + NADPH + H+ reaction in ether lipids biosynthesis Saccharomyces cerevisiae 1-alkyl-sn-glycerol 3-phosphate + NADP+
-
?
hexadecyldihydroxyacetone phosphate + NADPH
-
Saccharomyces cerevisiae 1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
?
palmitoylglycerone phosphate + NADPH i.e. palmitoyldihydroxyacetone phosphate Saccharomyces cerevisiae 1-palmitoylglycerol 3-phosphate + NADP+
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
40
-
-
Saccharomyces cerevisiae

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
28 43 about half-maximal activity at 28┬░C and 43┬░C Saccharomyces cerevisiae

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30
-
10 min stable below Saccharomyces cerevisiae
45
-
t1/2: 10 min Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 7.2 broad optimum Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH specific for NADPH Saccharomyces cerevisiae
NADPH hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates Saccharomyces cerevisiae