Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
apo-form complexed with NADPH, vapor diffusion method, using 10% (w/v) polyethylene glycol 4000, 10% (v/v) 2-propanol, 0.1 M HEPES pH 7.0 and 30% (v/v) jeffamine ED2001, 0.1 M HEPES pH 7.5 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
R14A | inactive | Saccharomyces cerevisiae |
S36A | the mutant shows reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
S40A | the mutant shows reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0205 | - |
NADPH | wild type enzyme, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
0.0264 | - |
NADPH | mutant enzyme S36A, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
0.0571 | - |
NADPH | mutant enzyme S40A, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
0.8158 | - |
acetoacetyl-CoA | wild type enzyme, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
0.8871 | - |
acetoacetyl-CoA | mutant enzyme S36A, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
0.9913 | - |
acetoacetyl-CoA | mutant enzyme S40A, at pH 8.0 and 25°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | Saccharomyces cerevisiae | - |
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P35731 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | - |
Saccharomyces cerevisiae | a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | - |
Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
3-oxoacyl-(acyl-carrier-protein) reductase | - |
Saccharomyces cerevisiae |
3-oxoacyl-ACP reductase | - |
Saccharomyces cerevisiae |
FabG | - |
Saccharomyces cerevisiae |
OAR1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
91.4 | - |
NADPH | mutant enzyme S40A, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
91.4 | - |
acetoacetyl-CoA | mutant enzyme S40A, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
108.2 | - |
NADPH | mutant enzyme S36A, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
108.2 | - |
acetoacetyl-CoA | mutant enzyme S36A, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
117.5 | - |
NADPH | wild type enzyme, at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
117.5 | - |
acetoacetyl-CoA | wild type enzyme, at pH 8.0 and 25°C | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Saccharomyces cerevisiae |