Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Rosetta 2 (DE3) pLysS cells | Mycolicibacterium smegmatis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, apoenzyme is crystallized by using 100 mM Na-HEPES (pH 7.5), 30% (w/v) PEG 400 and 200 mM magnesium chloride hexahydrate, while the complex with NADP+ is crystallized by using 200 mM sodium citrate tribasic dehydrate and 20% (w/v) PEG 3350 | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, SDS-PAGE | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+ | Mycolicibacterium smegmatis | - |
beta-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+ | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
beta-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R723 | - |
- |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | A0R723 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel Sepharose column chromatography and ENrich SEC 650 gel filtration | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | - |
Mycolicibacterium smegmatis | ? | - |
? | |
acetoacetyl-CoA + NADPH + H+ | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+ | - |
Mycolicibacterium smegmatis | beta-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+ | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | beta-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
additional information | no activity with NADH | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | no activity with NADH | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP reductase | - |
Mycolicibacterium smegmatis |
FabG | - |
Mycolicibacterium smegmatis |
ketoacyl-acyl carrier protein reductase | - |
Mycolicibacterium smegmatis |
MSMEG_6753 | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycolicibacterium smegmatis |