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Literature summary for 1.1.1.100 extracted from

  • Blaise, M.; Van Wyk, N.; Baneres-Roquet, F.; Guerardel, Y.; Kremer, L.
    Binding of NADP+ triggers an open-to-closed transition in a mycobacterial FabG beta-ketoacyl-ACP reductase (2017), Biochem. J., 474, 907-921.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Rosetta 2 (DE3) pLysS cells Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, apoenzyme is crystallized by using 100 mM Na-HEPES (pH 7.5), 30% (w/v) PEG 400 and 200 mM magnesium chloride hexahydrate, while the complex with NADP+ is crystallized by using 200 mM sodium citrate tribasic dehydrate and 20% (w/v) PEG 3350 Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
x * 100000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+ Mycolicibacterium smegmatis
-
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+ Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0R723
-
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0R723
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel Sepharose column chromatography and ENrich SEC 650 gel filtration Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + NADPH + H+
-
Mycolicibacterium smegmatis ?
-
?
acetoacetyl-CoA + NADPH + H+
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+
-
Mycolicibacterium smegmatis beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?
additional information no activity with NADH Mycolicibacterium smegmatis ?
-
?
additional information no activity with NADH Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP reductase
-
Mycolicibacterium smegmatis
FabG
-
Mycolicibacterium smegmatis
ketoacyl-acyl carrier protein reductase
-
Mycolicibacterium smegmatis
MSMEG_6753
-
Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Mycolicibacterium smegmatis