Literature summary for 1.1.1.10 extracted from

Li, S.; Ye, Z.; Moreb, E.A.; Hennigan, J.N.; Castellanos, D.B.; Yang, T.; Lynch, M.D.
Dynamic control over feedback regulatory mechanisms improves NADPH flux and xylitol biosynthesis in engineered E. coli (2021), Metab. Eng., 64, 26-40 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	xylitol is produced from xylose via the NADPH dependent reductase, a two-stage dynamic control over feedback regulatory mechanisms improves NADPH flux and xylitol biosynthesis in engineered Escherichia coli, method evaluation, overview. Comparison of two approaches to optimize xylitol biosynthesis: a stoichiometric approach wherein competitive fluxes are decreased, and a regulatory approach wherein the levels of key regulatory metabolites are reduced. The stoichiometric and regulatory approaches lead to a 20fold and 90fold improvement in xylitol production, respectively. Strains with reduced levels of enoyl-ACP reductase and glucose-6-phosphate dehydrogenase, lead to altered metabolite pools resulting in the activation of the membrane bound transhydrogenase and an NADPH generation pathway, consisting of pyruvate ferredoxin oxidoreductase coupled with NADPH dependent ferredoxin reductase, causing increased NADPH fluxes, despite a reduction in NADPH pools. These strains produce titers of 200 g/l of xylitol from xylose at 86% of theoretical yield in instrumented bioreactors	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
xylitol + NADP+	Escherichia coli	-	xylulose + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	A0A0E3ZP28	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
xylitol + NADP+	-	Escherichia coli	xylulose + NADPH + H+	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)