Application | Comment | Organism |
---|---|---|
synthesis | potential approach for industrial-scale production of xylitol from hemicellulosic hydrolysate involving the enzyme | Neurospora crassa |
Cloned (Comment) | Organism |
---|---|
gene xr, recombinant expression in Escherichia coli strain W3110 from p43-XR comprising a constitutive promotor and gene xr, subcloning in Escherichia coli strain DH5alpha, gene xr expression analysis by quantitative RT-PCR | Neurospora crassa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Escherichia coli strain WZ04 is first constructed by a simultaneous deletion-insertion strategy involving CRISPR/Cas9 markerless gene-editing technology, replacing ptsG, xylAB and ptsF in wild-type Escherichia coli strain W3110 with three mutated xylose reductase genes (xr) from Neurospora crassa. In a second approach, the pfkA, pfkB, pgi and/or sthA genes are deleted and replaced by xr to investigate the influence of carbon flux toward the pentose phosphate pathway and/or transhydrogenase activity on NADPH generation. The deletion of pfkA/pfkB significantly improves NADPH supply, but minimally influences cell growth. The effects of insertion position and copy number of xr are examined by a quantitative real-time PCR and a shake-flask fermentation experiment. In a fed-batch fermentation experiment with a 15-l bioreactor, strain WZ51 produces 131.6 g/l xylitol from hemicellulosic hydrolysate (xylitol productivity: 2.09 g/l/h). The biotransformation process involves resting cell catalysis with pure xylose as the substrate, but not hemicellulosic hydrolysate. Cofactor ratios are measured for single gene replacement strains and strains with combinatorial gene replacements, all based on strain WZ04, overview | Neurospora crassa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Neurospora crassa | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NADP+ | Neurospora crassa | - |
L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | Neurospora crassa CBS 708.71 | - |
L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | Neurospora crassa 74-OR23-1A | - |
L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | Neurospora crassa DSM 1257 | - |
L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | Neurospora crassa ATCC 24698 | - |
L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | Neurospora crassa FGSC 987 | - |
L-xylulose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | Q7SD67 | - |
- |
Neurospora crassa 74-OR23-1A | Q7SD67 | - |
- |
Neurospora crassa ATCC 24698 | Q7SD67 | - |
- |
Neurospora crassa CBS 708.71 | Q7SD67 | - |
- |
Neurospora crassa DSM 1257 | Q7SD67 | - |
- |
Neurospora crassa FGSC 987 | Q7SD67 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NADP+ | - |
Neurospora crassa | L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | - |
Neurospora crassa CBS 708.71 | L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | - |
Neurospora crassa 74-OR23-1A | L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | - |
Neurospora crassa DSM 1257 | L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | - |
Neurospora crassa ATCC 24698 | L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | - |
Neurospora crassa FGSC 987 | L-xylulose + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
XylB | - |
Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vivo assay at | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Neurospora crassa | |
NADPH | - |
Neurospora crassa |
General Information | Comment | Organism |
---|---|---|
physiological function | cofactor supply is a rate-limiting step in the bioconversion of xylose to xylitol | Neurospora crassa |