Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.10 extracted from

  • Khattab, S.M.; Saimura, M.; Kodaki, T.
    Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP+-dependent xylitol dehydrogenase (2013), J. Biotechnol., 165, 153-156.
    View publication on PubMed
Search for more literature for 1.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of strictly NADPH-dependent XR with mutated strictly NADP+-dependent XDH leading to increased bioethanol production and decreased xylitol accumulation in Saccharomyces cerevisiae strain D452-2 resulting in mutant strains SK-N1 to SK-N4 Scheffersomyces stipitis

Protein Variants

Protein Variants Comment Organism
additional information enzyme XDH is changed from NAD+-dependent to NADP+-dependent, xylitol accumulation is reduced and ethanol production improved using protein engineering for reversing the dependency of XDH from NAD+ to NADP+. Construction of a set of recombinant Saccharomyces cerevisiae carrying a mutated strictly NADPH-dependent XR and NADP+-dependent XDH genes with overexpression of endogenous xylulokinase (XK), effects of complete NADPH/NADP+ recycling on ethanol fermentation and xylitol accumulation, overview. The mutated strains demonstrate 0% and 10% improvement in ethanol production, and reduced xylitol accumulation, ranging 34.4-54.7% compared with the control strain Scheffersomyces stipitis

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis
-
 i.e Pichia stipitis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.11 15.29 recombinant strains harboring genes Xr and mutated XDH, pH and temperature not specified in the publication Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NADP+ engineered enzyme with altered cofactor specificity Scheffersomyces stipitis L-xylulose + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
NADP+-dependent xylitol dehydrogenase
-
 Scheffersomyces stipitis
XDH
-
 Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NADP+ engineered enzyme with altered cofactor specificity Scheffersomyces stipitis

General Information

General Information Comment Organism
metabolism the redox balance between xylose reductase (XR) and xylitol dehydrogenase (XDH) is thought to be an important factor in effective xylose fermentation Scheffersomyces stipitis
physiological function strictly NADPH-dependent XR with mutated strict NADP+-dependent XDH are more effective in increasing bioethanol production and decreasing xylitol accumulation than the wild-type, overview Scheffersomyces stipitis