Ligand tripolyphosphate

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Basic Ligand Information

Molecular Structure
Picture of tripolyphosphate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H5O10P3
tripolyphosphate
PRTGXBPFDYMIJH-MKQZUAMYSA-N
Synonyms:
(phosphate)3, Triphosphate, tripolyphosphates


Show all pahtways known for Show all BRENDA pathways known for tripolyphosphate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
triphosphate + [microsomal membrane protein] = diphosphate + phosphorylated [microsomal membrane protein]
show the reaction diagram
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triphosphate + H2O = 3 phosphate
show the reaction diagram
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triphosphate + H2O = 3 phosphate
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
dGTP + H2O = deoxyguanosine + triphosphate
show the reaction diagram
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7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (37 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
tripolyphosphate + H2O = diphosphate + phosphate
show the reaction diagram
triphosphate + 2'-deoxyguanosine = diphosphate + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
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triphosphate + NAD+ = diphosphate + NADP+
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triphosphate + 2'-deoxycytidine = diphosphate + 2'-deoxycytidine 5'-phosphate
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tripolyphosphate + inosine = ? + IMP
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triphosphate + D-fructose 6-phosphate = diphosphate + D-fructose 1,6-bisphosphate
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AMP + (phosphate)3 = ADP + (phosphate)2
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P1,P4-bis(5'-guanosyl)tetraphosphate + tripolyphosphate = GTP + ?
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UDP-glucose + tripolyphosphate = uridine-5'-tetraphosphate + D-glucose-1-phosphate
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triphosphate + [microsomal membrane protein] = diphosphate + phosphorylated [microsomal membrane protein]
show the reaction diagram
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triphosphate + H2O = diphosphate + phosphate
show the reaction diagram
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triphosphate + H2O = diphosphate + phosphate
show the reaction diagram
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tripolyphosphate + H2O = diphosphate + phosphate
show the reaction diagram
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triphosphate + H2O = diphosphate + phosphate
show the reaction diagram
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ATP + tripolyphosphate = adenosine 5'-tetraphosphate
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4-coumarate + MgATP2- + tripolyphosphate = adenosine 5'-tetraphosphate + ?
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ATP + tripolyphosphate = adenosine 5'-triphosphate derivative of tripolyphosphate + diphosphate
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Product in Enzyme-catalyzed Reactions (79 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
farnesyl triphosphate + [Ras]-Cys-Val-Ile-Met = triphosphate + [Ras]-S-farnesyl-Cys-Val-Ile-Met
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(phosphate)4 + D-glucose = (phosphate)3 + D-glucose 6-phosphate
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tetrapolyphosphate + NADH = tripolyphosphate + NADPH
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deoxythymidine 5'-tetraphosphate + alpha-D-glucose 1-phosphate = triphosphate + dTDP-alpha-D-glucose
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tetraphosphate + [microsomal membrane protein] = triphosphate + phosphorylated [microsomal membrane protein]
show the reaction diagram
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ATP + H2O = adenosine + tripolyphosphate
show the reaction diagram
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polyphosphate700 + H2O = phosphate + triphosphate
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Activator in Enzyme-catalyzed Reactions (7 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulates light production under some conditions
-
10 mM, stimulates
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activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration
activation, can replace ATP
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Inhibitor in Enzyme-catalyzed Reactions (33 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
when present in excess together with Graham‘s salt at pH 7.2
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10 mM, 54% inhibition
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99.9% inhibition at 1 mM
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0.6 mM, 50% inhibition of GTP cyclohydrolase activity, 0.05 mM, 52% inhibition of diphosphate phosphohydrolase activity
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inhibits the endopolyphophatase of the recombinant enzyme DDP1
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competitive inhibitor of adenosine tetraphosphate
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inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1
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inhibits the endopolyphophatase of the recombinant enzyme DDP1
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Metals and Ions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE

3D Structure of Enzyme-Ligand-Complex (PDB) (55 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (38 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
3
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
0.0062
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in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C

KM Value (56 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.092
-
pH 7.5, 37°C
2.4
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
0.074
-
pH 7.5, 37°C, with deoxycytidine
4.5
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30°C, pH 8.0
4.7
-
synthesis of adenosine 5´-tetraphosphate
1.3
-
in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C

Ki Value (6 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.143
-
versus ATP, with substrate 2'-deoxycytidine
0.014
-
pH 7.5, 30°C

IC50 Value (1 result)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.00002
-
recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C

References & Links

Links to other databases for tripolyphosphate

ChEBI
PubChem
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PubChem