Ligand ADP-ribose

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Basic Ligand Information

Molecular Structure
Picture of ADP-ribose (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C15H23N5O14P2
ADP-ribose
SRNWOUGRCWSEMX-KEOHHSTQSA-N
Synonyms:
5-(adenosine-5'-diphosphoryl)-D-ribose, A-Rib-5'-P-P-5'-Rib, adenosine-5'-diphosphoribose, adenosine 5'-diphosphoribose, adenosine diphosphate ribose, adenosine diphosphoribose, ADP-D-ribose, ADPribose, ADP ribose, beta-ADP-ribose
Pathway Source
Pathways
MetaCyc


Show all pahtways known for Show all BRENDA pathways known for ADP-ribose

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (44 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
phosphate + ADP-ribose = ADP + D-ribose 5-phosphate
show the reaction diagram
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diphosphate + ADP-D-ribose = ATP + D-ribose 5-phosphate
show the reaction diagram
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ADP-ribose + H2O = AMP + D-ribose 5-phosphate
show the reaction diagram
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ADP-ribose + H2O = AMP + phosphoribose
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (25 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + D-ribose 5-phosphate = diphosphate + ADP-D-ribose
show the reaction diagram
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poly(ADP-D-ribose)n + H2O = poly(ADP-D-ribose)n-1 + ADP-ribose
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (58 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
phosphate + ADP-ribose = ADP + D-ribose 5-phosphate
show the reaction diagram
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diphosphate + ADP-D-ribose = ATP + D-ribose 5-phosphate
show the reaction diagram
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ADP-ribose + H2O = ?
show the reaction diagram
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3-acetylpyridine + adenosine diphosphoribose = 3-acetylpyridine adenine dinucleotide + phosphate
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ADP-ribose + H2O = IDP-ribose + NH3
show the reaction diagram
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ADPribose + H2O = IDPribose + NH3
show the reaction diagram
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ADP-ribose + H2O = AMP + alpha-D-ribose 1-phosphate
show the reaction diagram
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ADP-ribose + H2O = ?
show the reaction diagram
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ADP-ribose + H2O = ?
show the reaction diagram
-
ADP-ribose + H2O = AMP + D-ribose 5-phosphate
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (55 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + D-ribose 5-phosphate = diphosphate + ADP-D-ribose
show the reaction diagram
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PARP10-Nomega-(ADP-D-ribosyl)-L-arginine + H2O = ADP-D-ribose + PARP10-L-arginine
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activity is increased several-fold upon preincubation with submicromolar concentrations of ADPribose. Activation is enhanced by the presence of ATP. ADP-ribose acts by evoking a conformational transition in the enzyme. Other mono- and di-nucleotides are ineffective
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Inhibitor in Enzyme-catalyzed Reactions (72 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
; coenzyme-competitive inhibitor
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competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate
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competitive inhibition vs. coenzyme, noncompetitive vs. substrate
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weak inhibitor
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coenzyme analogue with a non-cooperative behaviour of binding, is a potent competitive inhibitor
at high concentration, competitive with respect to NADPH
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partial competitive inhibition
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3.5 mM, 31% inhibition
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competitive to phosphate
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strong inhibition
competitive to diuridine tetraphosphate
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addition of 1-1.4 mM, inhibition 74.4%
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inhibits cleavage of 8-oxo-dGTP
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47% inhibition at 0.5 mM, competitive type of inhibition
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reductive inhibition
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3D Structure of Enzyme-Ligand-Complex (PDB) (35 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (111 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
3.6
-
pH 8.2, 37°C
2.07
-
at pH 5.0 and 37°C
370
-
pH 6.0, 37°C

KM Value (130 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.041
-
pH 8.2, 37°C
0.348
-
at pH 5.0 and 37°C
0.125
-
pH 7.5
0.3
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in 50 mM Tris-HCl, pH 8.0, 10 mM Mg2+, at 37°C

Ki Value (21 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.95
-
pH 7.8, 23°C
0.52
-
pH 7.8, 25°C, substrate: NAD+
0.29
-
at pH 7.0
0.2
-
inhibition of the recombinant FAD domain
0.03
-
in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C
0.023
-
pH 7.4, 37°C
1.1
-
-
0.002
-
competitive inhibition
3.6
-
pH 7.4, 37°C, recombinant wild-type enzyme

IC50 Value (2 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.012
-
at pH 7.0 and 30°C
0.228
-
pH 7.4, temperature not specified in the publication

References & Links

Links to other databases for ADP-ribose

ChEBI
PubChem
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PubChem