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Ligand Raucaffricine

Basic Ligand Information

Molecular Structure

Related pathways

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.125

raucaffricine + H2O = D-glucose + vomilenine

488937, 488938, 732619, 751210, 774241

-

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.219

UDP-glucose + vomilenine = UDP + raucaffricine

-

-

Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.125

raucaffricine + H2O = D-glucose + vomilenine

488937, 488938, 682286, 713606, 732619, 751210, 774241

-

3.2.1.125

raucaffricine + H2O = vomilenine + D-glucose

488934

-

3.2.1.125

2 entries

Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.219

UDP-glucose + vomilenine = UDP + raucaffricine

-

-

Enzyme Kinetic Parameters

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.219

1.3

-

-

488938

3.2.1.125

1.3

-

-

682286

3.2.1.125

1.3

-

pH 5, 28°C, recombinant RG

488938

3.2.1.125

1.3

-

wild type enzyme, pH and temperature not specified in the publication

713606

3.2.1.125

1.4

-

-

488934

3.2.1.125

4 entries

References & Links

Literature References (8)

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Show Reference (8 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

488934

-

Partial purification and characterization of raucaffricine beta-D-glucosidase from plant cell-suspension cultures of Rauwolfia serpentina Benth

1986

Schuebel, H.; Stoeckigt, J.; Feicht, R.; Simon, H.

Helv. Chim. Acta

69

538-547

488937

10234858

Purification, partial amino acid sequence and structure of the product of raucaffricine-O-beta-D-glucosidase from plant cell cultures of Rauwolfia serpentina

1999

Warzecha, H.; Obitz, P.; Stockigt, J.

Phytochemistry

50

1099-1109

488938

10975500

Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis

2000

Warzecha, H.; Gerasimenko, I.; Kutchan, T.M.; Stockigt, J.

Phytochemistry

54

657-666

682286

-

The molecular architecture of major enzymes from ajmaline biosynthetic pathway

2007

Stoeckigt, J.; Panjikar, S.; Ruppert, M.; Barleben, L.; Ma, X.; Loris, E.; Hill, M.

Phytochem. Rev.

6

15-34

713606

22004291

Structures of alkaloid biosynthetic glucosidases decode substrate specificity

2012

Xia, L.; Ruppert, M.; Wang, M.; Panjikar, S.; Lin, H.; Rajendran, C.; Barleben, L.; Stoeckigt, J.

ACS Chem. Biol.

7

226-234

732619

22704651

High speed X-ray analysis of plant enzymes at room temperature

2013

Xia, L.; Rajendran, C.; Ruppert, M.; Panjikar, S.; Wang, M.; Stoeckigt, J.

Phytochemistry

91

88-92

751210

25140865

Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding and inhibitory behaviours

2015

Xia, L.; Lin, H.; Staniek, A.; Panjikar, S.; Ruppert, M.; Hilgers, P.; Williardt, J.; Rajendran, C.; Wang, M.; Warzecha, H.; Jäger, V.; Stöckigt, J.

J. Enzyme Inhib. Med. Chem.

30

472-478

774241

32314186

A beta-glucosidase gene from Stevia rebaudiana may be involved in the steviol glycosides catabolic pathway

2020

Yang, Y.; Hou, M.; Zhang, T.; Sun, Y.; Zhang, Y.; Huang, S.; Xu, X.; Yuan, H.

Mol. Biol. Rep.

47

3577-3584

Links to other databases for Raucaffricine

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Show Links (1 entry)