Ligand ammonium sulfate

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Basic Ligand Information

Molecular Structure

H₈N₂O₄S

ammonium sulfate

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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Synonyms:

(NH4)2SO4, ammoniumsulfate, diammonium sulfate

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (1 result)

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4.1.1.90

FLEEL + CO2 + O2 + ammonium sulfate = ? + vitamin K epoxide + H2O

701214

Product in Enzyme-catalyzed Reactions (1 result)

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4.98.1.1

mesoporphyrin IX + ferrous ammonium sulfate = mesoheme + ammonium sulfate

Activator in Enzyme-catalyzed Reactions (70 results)

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1.1.1.188

activity of the purified recombinant enzyme is stimulated about 4fold by 1.5 M

about 100% activation at 10 mM

668061

1.1.1.95

enhancing effect on NAD-dependent 3-phospho-D-glycerate oxidation and 3-phosphohydroxypyruvate reduction but inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction

additions of ammonium sulfate even at weak concentrations (10 to 70 mM) stimulate catalatic activity measured in the presence of 1.5 M NaC1 by about 30%

714289

1.12.98.1

improves reactivation of enzyme with FAD after FAD depletion

activates, optimal concentration is 1.25 mM

the enzyme activity is increased about 10fold in the presence of 0.045 M (NH4)2SO4

stimulates activity

short incubation activates isozyme E-II, not E-I

stimulates

250 mM, 10fold activation

721365

1.8.4.9

less effective than Na2SO4

288640

2.1.1.45

10 mM, relative activity 108%

485363

2.3.1.20

45 mM, 10% increase in activity

659060

2.3.1.75

45 mM, 10% increase in activtiy

activates at 0.5 mM

maximum of activity: about 45% (NH4)2SO4 and decrease in activity at higher concentrations

10 mM, activates

stimulation

activates

high salt activates at high adenosine 5'-phosphosulfate concentrations but inhibits at low adenosine 5'-phosphosulfate concentrations

641223

2.7.2.3

inhibition at high concentration, acceleration of activity at low concentrations

inhibitory above 30 mM, activates below

stimulates

optimal concentration of 20 mM, inhibition below and above 20 mM

500 mM, activates

with FeSO4, when used as nitrogen source, 1.7fold increase in activity

10 mM, 127% of initial activity, 50 mM, no residual activity

xylanase activity is 9.7 U/ml with 0.1% urea

695683

3.5.1.28

20% activation at 0.15 M

172011

3.5.1.71

0.4 M, enhances activity of unpurified enzyme by 55%, but no effect with purified enzyme

209231

3.5.4.12

substrate dCMP-Hg-S-CH2-CH2-OH, 0.2 M ammonium sulfate: activation, reversed by cCTP

50 mM, stimulates

1 mM, 203% of initial activity

20% stimulation at concentrations above 25mM

651729

6.5.1.2

with 30 mM (NH4)2SO4, the activity is maximal

715672

Inhibitor in Enzyme-catalyzed Reactions (149 results)

0.05 mM: slight inhibition, 400 mM: activation

286245, 286253

1.1.1.37

reduction of oxaloacetate to malate and oxidation of malate to oxaloacetate

656320

1.1.1.40

at high concentrations

1 M, 71% inhibition

100 mM, 68% inhibition, 10 mM, 8% inhibition

287523

1.1.1.95

inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction

slightly inhibits

10 mM

more than 50% inhibition at 25 mM

mutant enzyme is less sensitive to inhibition than wild-type enzyme

enzyme activity is inhibited by ammonium sulfate ; however, this inhibition is overcome by addition of 10 mM guanidine

1.9 M, E II: activation at short incubation time, E I: complete inactivation after 10 h incubation; inactivation of isozyme E-I after 10 h

391881

1.5.1.17

200 mM, 16% inhibition

1 mM, 71% inhibition

0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory

slightly inhibitory

20% activity at 0.5 M

strong inhibitor at 1 mM

486807

2.3.1.48

competitive against both acetyl-CoA and histones

50-300 mM, weak inhibition

488295

2.4.1.11

20% inactivation at 20 mM

1 mM

1 M, about 50% inhibition

636814

2.4.1.236

inhibits the activity of the partially purified enzyme

weak

at high concentration

0.05 mM, 45% inhibition, 5 mM, complete inhibition

slightly inhibitory

50% inhibition at 200 mM

641140

2.7.1.25

high salt inhibits at low adenosine 5'-phosphosulfate concentrations, but activates at high adenosine 5'-phosphosulfate concentrations

inhibits at high concentrations

5 mM, 29% inhibition

above 0.2 M, stimulates at 0.05-0.1 M

above 30 mM, activates below

642600

2.7.4.33

slightly inhibitory at 50 mM

722513

2.7.4.9

0.35 M, 50% inhibition

inhibits both subunit GlgC and GlgC/GlgD complex

739100

2.7.7.43

1.0 M, 83% inhibition, reversible by desalting

above 60 mM

optimal concentration of 20 mM, inhibition below and above 20 mM

5 mM, 71% of initial activity

772614

3.1.1.31

0.06 M, 55% inhibition

0.05 M

50% inhibition at 25 mM, inhibition of formation of acid-soluble products

288787

3.1.21.9

0.05 M, 70% inhibition

10 mM, 127% of initial activity, 50 mM, no residual activity

666587

3.1.3.22

complete inactivation with higher concentrations

94719

3.1.3.25

30% inhibition at 10 mM

slight

0.5 M, 40% inhibition

intracellular enzyme

0.5 mM, 36% residual activity

655828

3.2.1.68

0.5 M, 30% residual activity

76% inhibition at about 40 mM

inhibition above 10 mM

209100

3.5.5.1

75% residual activity at 800 mM

684594

4.1.1.15

92% inhibition at 2 mM

10 mM, slight

62% inhibition at 80.6 mM, complete inhibition at 806 mM

648385

4.2.1.33

recovery after restoration of lower ionic strength shows hysteresis effect

50% inactivation at 400 mM

648564

4.4.1.13

66% inhibition at 4 mM

691730

5.1.1.17

100 mM, 47% inhibition

weak, gamma-glutamyl transferase activity

37486

6.3.1.9

almost complete inhibition at 500 mM

25 mM

10 mM, 60% inhibition

653383

6.5.1.2

(NH4)2SO4 concentrations higher than 30 mM are inhibitory. At 100 mM KCl, concentrations of (NH4)2SO4 above 12 mM are inhibitory

715672

Metals and Ions (41 results)

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1.1.1.47

NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion

4fold activation

1.5 M, stimulates about 4fold

687797

1.13.11.49

activity increases with increasing salt concentration up to 2 M

697991

1.13.11.6

the most effective salt, the optimal concentration is 0.035 M

439364

1.2.1.12

0.045 M, 10fold activation

287938, 287968

1.3.98.1

enhances enzymatic activity up to a concentration of 0.0014 mM

activates

the activity of the recombinant methylase in 0.5 M and 1.0 M ammonium sulfate is about 1.25fold higher than in the absence of ammonium sulfate

activates at at least 0.5 mM

488034

2.4.1.187

0.3-0.5 M: stimulation

activates

activation, 0.05-0.1 M, inhibits above 0.2 M

slightly stimulating

activates

activates optimally at 0.2 M

677887

3.1.3.63

activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2

646443

3.1.3.72

0.166 M, enhances activity

slight activation

high salt concentration, (NH4)2SO4, 2 M, dissociates the high molecular weight form yielding the low molecular form and increasing the specific activity

285237

3.12.1.B1

1 M, 4fold increase in activity

756219

3.2.1.147

activation is stronger in presence of 1 mM ascorbate

208560

3.2.1.73

activation to 105.5% at 5 mM

(NH4)2SO4 enhances autoconversion of the enzyme, it increases formation of the mature (M) form, after 2 h incubation all molecules are converted to the M-form

775632

3.5.3.6

optimal activity in presence of 2.0 M, 48% of the activation with NaCl

relative activity: 409%

693771

6.2.1.44

0.4 M, 1.5fold enhancement of activity

optimal up to 50 mM

stimulation by 5-25 mM

1289

7.2.1.4

2-3fold activation at 1 M

770925

Enzyme Kinetic Parameters

K_i Value (5 results)

0.05

pH 6.7, 85°C

0.85

2.5

pH 7.5, 30°C

666053

IC₅₀ Value (2 results)

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2.7.1.78

2 entries

References & Links

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Show Reference (242 entries)

Links to other databases for ammonium sulfate

Ligand ammonium sulfate

Basic Ligand Information

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (1 result)

Product in Enzyme-catalyzed Reactions (1 result)

Activator in Enzyme-catalyzed Reactions (70 results)

Inhibitor in Enzyme-catalyzed Reactions (149 results)

Metals and Ions (41 results)

Enzyme Kinetic Parameters

Ki Value (5 results)

IC50 Value (2 results)

References & Links

Literature References (242)

Links to other databases for ammonium sulfate

K_i Value (5 results)

IC₅₀ Value (2 results)