Ligand mercury(2+)

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Basic Ligand Information

Molecular Structure

mercury(2+)

BQPIGGFYSBELGY-UHFFFAOYSA-N

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Synonyms:

cationic mercury, Hg2+

Related pathways

BRENDA

phenylmercury acetate degradation

MetaCyc

phenylmercury acetate degradation

Show all BRENDA pathways known for mercury(2+)

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Roles as Enzyme Ligand

ATP + H2O + Hg2+/in = ADP + phosphate + Hg2+/out

246929

Product in Enzyme-catalyzed Reactions (57 results)

ATP + H2O + Hg2+/in = ADP + phosphate + Hg2+/out

246929

Activator in Enzyme-catalyzed Reactions (5 results)

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1.1.3.4

1 mM, about 85% inhibition

mercury chloride, and methylmercury, activates PLD, the stimulation is regulated by Ca2+ and calmodulin, mechanism, overview. Calcium chelating agents and calcium depletion, e.g. by EGTA, calmodulin inhibitors, e.g. calmidazolium chloride and trifluoperazine, and L-type calcium channel blockers nifedipine, nimodipine, and diltiazem attenuate the stimulation of PLD by mercury, overview

708801

3.2.1.35

enhances the activity of the enzyme

751278

3.4.22.2

papain activity increases to a maximum of 111.03% (non-competitive type activation) at a concentration of 0.000001x01mol/l Hg2+, but is almost completely deactivated at concentrations above 0.0001 mol/lx01Hg2+. The inhibition of Hg2+ on papain is a competitive and uncompetitive mixed type inhibition

755374

Inhibitor in Enzyme-catalyzed Reactions (3534 results)

complete inhibition at 1 mM

0.005 mM, complete inhibition, prevented by 10 mM glutathione

40% inhibition of NAD dehydrogenase II at 0.004 mM

286004

1.1.1.151

53% inhibition at 0.002 mM

IC50 0.00009 mM

irreversible inactivation

complete inhibition at 2 mM

strong inhibition

nearly complete inhibition at 2 mM

complete inhibition at 1 mM

740216

1.1.1.203

complete inhibition at 1 mM

286230

1.1.1.206

99% inactivation at 1 mM

348024

1.1.1.207

almost complete inhibition at 0.25 mM

347974

1.1.1.208

almost complete inhibition at 0.25 mM

347974

1.1.1.215

complete inhibition

286273, 286274, 286275

slight inhibition

strong inhibitor

1 mM, less than 10% residual activity

724428

1.1.1.239

0.1 mM, complete inhibition

strong

inhibition at 1 mM, and destabilization at 10 mM

656326

1.1.1.301

2 mM, complete inactivation

667463

1.1.1.306

complete inhibition at 0.1 mM

288324

1.1.1.307

0.001 mM, 2 min, complete inhibition

696073

1.1.1.31

complete inhibition at 1 mM

completely inhibits reduction of 2-dehydropantolactone

0.0005 mM, almost complete inhibition

complete inhibition at 1 mM

100% inhibition at 5 mM; 5 mM, 100% inhibition

30% inhibition at 1 mM

760738

1.1.1.81

0.1 mM, complete inhibition

287379

1.1.1.B3

67% inhibition at 1 mM

1 mM, complete inhibition

729053

1.1.1.B65

strong inhibition at 1 mM

strong inhibitor

0.1 mM HgCl2 complete inhibition

1 mM, 93% inhibition

22% inhibition at 2 mM

1 mM, complete inhibition

0.1 mM concentration 98% inhibition

636324

1.1.3.B4

60% inhibition at 1 mM

1 mM, complete inhibition

2% residual activity at 1 mM

725738

1.1.99.18

2 mM, 20% inhibition after 6 h

100% inhibition at 1 mM

strong

100% inhibition at 0.1 mM

0.01 mM, appreciable inhibition

21% inhibition at 0.01 mM

inhibition of NADH oxidizing activity

394613

1.11.1.6

10 mM, 14% residual activity

complete inhibition at 1 mM

complete inhibition at 1 mM

0.1 mM, 45% inhibition

439502

1.13.11.39

1 mM, complete inhibition

strong inhibition

inhibition reversed by glutathione

1 mM, 87% loss of activity

0.4 mM, 96% inhibition

0.5 mM, strong

causes a 20-30% fall in activity

439240

1.14.13.10

50% inhibition at 0.005 mM

1 mM, 98% inhibition

0.01-0.1 mM, 96% loss of activity

707605

1.14.13.131

1 mM, about 5% residual activity

715375

1.14.13.2

0.1 mM HgCl2, complete inhibition

0.1 mM, 87% inhibition

749641

1.14.13.25

complete inhibition at 0.01 mM

438947

1.14.13.27

complete inhibition at 0.1 mM

438911

1.14.13.29

63% inhibition at 1 mM, crude enzyme extract

82.05% inhibition at 0.03 mM

742395

1.14.14.110

complete inhibition at 5 mM

723478

1.14.14.173

87% inhibition at 0.1 mM

severely inhibits enzyme activity

741685

1.14.15.3

0.1 mM concentration, 100% inhibition

0.0001 mM, 56% inhibition

slight inhibition

DELTA12-desaturase system, enzymatic complex

659830

1.14.20.13

0.4 mM, 96% inhibition

0.77% residual activity at 1.0 mM

743629

1.17.1.4

complete inhibition of hypoxanthine oxidation at 0.1 mM

strong inhibitor

62% inhibition at 1 mM

almost complete inhibition at 1 mM HgCl2

complete inhibition at 1 mM

77% inhibition in the presence of 1 mM

complete inhibition at 2 mM

1 mM causes complete inhibition

1 mM, 17.5% inhibition

complete inhibition at 1 mM

both isoforms

1 mM, 12 h, 4°C, 99% loss of activity

724216

1.2.1.99

1 mM, complete inhibition

mM concentration

strong inhibitor, 4% residual activity at 2 mM Hg2+

719637

1.21.3.8

complete inhibition at 2 mM

719786

1.21.4.5

severely inhibited by 1 mM

complete inhibition of both orotate reductase and NADH oxidase reaction

1 mM, 86% inhibition

0.1 mM, 92% inhibition

390649

1.3.1.31

1 mM, 97.9% loss of activity

complete inhibition at 1 mM

1 mM, 97% inhibition

completely inhibits at 0.1 mM

1 mM, 61% inhibition of the recombinant enzyme

1 mM, 29% residual activity

763413

1.4.3.10

inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme

complete inhibition at 10 mM

2.0 mM

almost total inhibition at 0.1 mM

0.13 mM, complete inhibition

94167

1.5.1.36

strong inhibition at 1 mM Hg2+

89% inhibition at 1 mM

10 mM, complete loss of activity

765030

1.5.3.5

1 mM, about 80% inhibition

741686

1.5.8.2

strong inhibition at 1 mM

1 mM

96% inhibition at 0.1 mM

complete inhibition at 0.1 mg/ml

743751

1.6.3.3

0.1 mM, complete inhibition

0.5 mM, complete inhibition

about 70% inhibition

about 70% inhibition

80% inhibition at 1 mM

inhibitory

relative activity less than 5%

strong inhibition

1 mM shows strong inhibitory effect on recombinant rBfmBC activity (more than 80% inhibition)

0.01 mM, 100% inhibition

1 mM, 12.4% residual activity

764372

1.97.1.9

complete inhibition at 1 mM

complete inhibition

0.1 mM, complete inhibition

485119

2.1.1.121

5 mM, complete inhibition

0.1 mM, 96% inhibition

657589

2.1.1.232

5 mM, complete inhibition

713321

2.1.1.239

0.1 mM, strong inhibition

715347

2.1.1.25

0.1 mM, no resiudal activity

0.01 M, strong

0.5 mM Hg2+ ions totally inhibit the enzyme, even in the presence of 2 mM dithioerythritol

complete inhibition at 1 mM

441551

2.1.1.68

relative activity 0% of control

100% inhibition at 0.05 mM

0.1 mM, 43% residual activity

complete inhibition

0.1 mM, 65% inhibition

657044

2.3.1.172

0.1 mM, complete inhibition

0.00001 mM, 50% inhibition, noncompetitive, irreversible, probably due to formation of a thiolate

486657

2.3.1.272

complete inhibition at 0.1 mM

747307

2.3.1.28

0.01 mM, complete inhibition

strong

66% decrease of activity at 0.01 mM

strong inhibition

19.8% residual activity after 1 h at 1 mM

11% remaining activity isoenzyme Nat-b; 36% remaining activity isoenzyme NAT-a

687848

2.3.1.6

noncompetitive with respect to choline, IC50: 0.0004 mM, mixed type inhibition with respect to acetyl-CoA, IC50: 0.0025 mM, activity can be recovered using 2,3-dimercapto-propanol

complete inhibition

5 mM, complete inhibition

95% inhibition at 2 mM

735799

2.3.3.3

complete inactivation

1 mM

0.1 mM, residual activity 26%

674845

2.4.1.173

strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.5

672427

2.4.1.185

enzyme activity is completely inhibited by 0.1 mM Hg2+

at 1 mM

10 mM, complete inhibition, reversed by addition of 20 mM 2-mercaptoethanol

393094

2.4.1.219

complete inhibition at 10 mM

1 mM, 99% inhibition with quercetin as substrate

0.1 mM, complete inhibition. The observed enzyme inhibition by Cu2+ and Hg2+ may not solely be attributed to their effects on the enzyme itself because these heavy metal ions are known to destroy substrate anthocyanins

complete inhibition at 5 mM

Hg2+ significantly inhibits both enzyme activities, but especially the transferase activity (complete inhibition at 1 mM)

774147

2.4.1.49

slight inhibition at 1 mM

10 mM, complete inhibition

489343

2.4.1.8

1 mM, 50°C, 30 min, abolishes the phosphorolytic activity almost completely

1 mM, strong

97% inhibition at 1 mM

complete inhibition at 3 mM after 3 min at 0°C

complete inhibition at 0.1 mM

complete inhibition at 1 mM

strongly inhibites both O-acetyl-L-serine sulfhydrylation and O-phospho-L-serine sulfhydrylation

weak

1 mM, 0% residual activity

strong inhibition, reducing agent reverses

strong

0.002 mM, 50% inhibition in the presence of 3 mM Mg2+

0.01 mM, 25% inhibition, 0.1 mM, almost complete inhibition

55% inhibition at 1 mM

642033

2.7.10.2

at PO2 of about 20 mM

675624

2.7.2.11

0.1 mM, complete inhibition

0.083 mM, 76% inhibition

strong inhibition

partially reversed by dithiothreitol

645350

2.7.8.5

strong inhibition at concentrations above 0.5 mM in presence of Mg2+

1 mM, almost complete inhibition

10 mM, complete inhibition

657791

2.8.2.22

50% inhibition at 0.001 mM

1 mM, no residual activity

30% residual activity at 1 mM

716353

3.1.1.25

2.1% residual activity at 1 mM

713789

3.1.1.27

0.1 mM, complete inhibition

5 mM

6% remaining activity after 5 min, 1 mM

9% relative residual activity

1 mM, 58% inhibition

no activity at 1 mM HgCl2

134103

3.1.16.1

with RNA core as substrate

134135

3.1.2.12

susceptible to inhibition by Hg2+

50% inhibition at 0.0006 mM, inhibition of both DNA glycosylase and apurinic nicking activities, binding capability not reduced

0.1 mM, no residual activity

competitive towards Mg2+

competitive

reduces activity by 30-40%

strong inhibition at 1 mM

mild inhibition

1 mM, complete inhibition

potent inhibitor, inhibition reversed by adding an excess of dithiothreitol

strong

strong

1.33 mM, 1.2% relative activity

strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate

1 mM, 47.47% of initial activity

775444

3.2.1.137

98% inhibition at 1 mM

strong

inhibits activity at 1 mM, 6% relative activity compared with activity without any addition of effector

1 mM complete loss of activity

742545

3.2.1.178

10.37% residual activity at 5 mM

1 mM, 77% inhibition

1 mM, 88% loss of activity

723361

3.2.1.188

complete inhibition at 1 mM

726113

3.2.1.189

10 mM, significant inhibition

722930

3.2.1.191

10 mM, complete inhibition

1 mM, no residual activtiy

738475

3.2.1.196

about 40% residual activity at 5 mM

5 mM, 25% loss of activity

740658

3.2.1.206

no activity is detected after 1 h of incubation at 1mM Hg2+

0.1 M, 80% loss of activity

1 mM, 3% of initial activity

complete inhibition at 5 mM

noncompetitive

5 mM, strong inhibition

5 mM complete inactivation

136758, 136762, 136763, 136766, 136767, 136768, 136771

208766, 208767, 208775

3.2.1.72

more than 90% inhibition

136847, 136854, 136856, 136857, 136862

5 mM, complete inhibition

666900

3.2.1.89

136936, 136943, 136951

1 mM, complete inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis and 4-nitrophenyl beta-D-glucopyranoside hydrolysis

725057

3.2.1.B50

65% inhibition at 5 mM

1 mM,61% inhibition

35862, 35908, 95195, 95198, 95205

99% inhibition at 1 mM, recombinant enzyme

36044, 36057, 36061

strong, reversible by dialysis against 2 mM DTT

1 mM, complete inhibition, IC50: 0.67 mM

647189, 647195, 647213, 647224

100% inhibition at 1 mM

1 mM, 90% inhibition

36459, 36470, 36481

1 mM, complete inhibition

26% inhibition of amidolytic activity at 1 mM

inhibition of amidolytic activity

1 mM, 50% inhibition

29685, 29691, 29692

1 mM, strong inhibition

665085

3.4.21.B24

order of decreasing inhibitory effect: Cu2+, Hg2+, Zn2+, Ni2+, Co2+

649673

3.4.22.1

34989, 35024, 36631, 36638, 36639, 36640, 36641, 36647, 36651, 36652, 36653

0.1 mM: 85.5% decrease of activity

1 mM, almost complete inhibition

25.27% residual activity at 5 mM

708216

3.4.22.41

0.81% residual activity at 5 mM

5 mM completely inhibits

inhibitory below 5 mM

647645

3.4.23.18

74.8% residual activity at 10 mM

weak

0.001-0.05 mM, modifies the recombinant enzyme conformation, and highly reduces the enzyme activity. Hg2+ incubation increases NEP protein levels, but does not change NEP mRNA levels nor the levels of the amyloid intracellular domain peptide, a protein fragment with transcriptional activity. The Hg2+-induced inhibition of the enzyme activity may be mediated by a conformational change resulting in reduced amyloid beta1-42 degradation

protease II

complete inhibition at 0.05 mM

5 mM, 15% inhibition

1 mM, complete inhibition

692156

3.5.1.105

1 mM HgCl2, complete inhibition

696801

3.5.1.11

decreases enzyme activity to 50%

171903

3.5.1.12

171928, 171929, 171933

288855, 288863, 31930

172002, 172019, 172020, 172021, 172026

0.5 mM, complete inhibition

52% inhibition at 1 mM

complete inhibition at concentrations above 15 mM

0.01 mM, 15 min, 82% activity remains

31891, 31893, 31894

31914, 31920, 31923, 734635

complete inhibition at 1 mM

complete inhibition

2 mM, complete inhibition

652350

3.5.2.10

complete inhibition at the ionic strength of 0.1 mM HgCl, 20-30% activity restored at 20 mM 2-mercaptoethanol

172083

3.5.2.12

1 mM, complete enzyme inhibition

0.1 mM

0.2 mM, 87% inhibition

209545, 209546, 209548, 209550, 209551, 209553, 209559

3.5.4.17

0.5 mM, complete inhibition of deamination of ATP

0.01 mM

98.6% inhibition at 2 mM

5 mM, reduces activity by 78.8%

10 mM, inhibition 39%

2-mercaptoethanol partially protects

slightly inhibits

209779, 209780, 209787, 209790, 209799, 657244

0.2 mM

98% inhibition at 1 mM

1-5 mM, strong inhibition

strong

0.15 mM, 50% inhibition

10 mM, complete inhibition

0.1 mM, strong inhibition

5 mM, in presence of 5 mM Mg2+

648364, 648365, 648366

4.1.1.86

over 90% inhibition at 1 mM

86% inhibition at 1.08 mM, complete inhibition at 10.8 mM

648385

4.1.2.43

81% inhibition with 1 mM Hg2+

691118

4.1.2.45

1 mM, 40% inhibition of fHBP HA B

10 mM, in presence of 2.5 mM Mg2+, 30% inhibition

complete inhibition at 1 mM

leads to complete inhibition at 10 mM

716180

4.2.1.143

1 mM, complete loss of activity

722606

4.2.1.22

reactivity of Co(III) hCBS with HgCl2 is consistent with a loss of the cysteine(thiolate) ligand. 2-Mercaptoethanol is unable to reverse the Hg-induced ligand switch, in contrast to some other heme-thiolate proteins

0.1 mM HgCl2, apoenzyme: complete loss of activity, inactive enzyme complex with hydroxocobalamin: 70% loss of activity

5750, 5758, 5760

0.1 mM, 94% inhibition

1 mM, complete inactivation

650299

4.2.2.14

94% reduced activity at 1 mM

1 mM, strong

both enzyme forms IM3796 and IM1634 are completely inhibited by 5 mM Hg2+

772952

4.2.2.21

about 90% residual activity at 5 mM

1 mM, no residual activity

strongly inhibited (to 50%) by 10 mM Hg2+

1 mM

1 mM, 96% inhibition

0.2 mM, 99% inhibition

complete

648741, 648743, 768494

4.4.1.2

10 mM, 95% loss of activity

10 microM 100% inhibition, 1 microM 67% activity left

34834, 34849, 34850, 34854

1 mM, complete inactivation

strong inhibition

complete inhibition at 1 mM

748352

5.1.1.17

1 mM, complete inhibition

complete inhibition, recombinan t enzyme

1 mM, about 90% inhibition

weak

5% residual activity at 1 mM

MCM activity is inhibited completely by the addition of 3 mM Hg2+

about 45% residual activity at 1 mM

inhibits ATPase activity, IC50: 49 nM, targets the cysteine residue in the DECH box, competitive, cysteine or DTT protect at large concentrations

10 mM, complete inhibition in presence of 10 mM Mg2+, no activation in absence of Mg2+

589, 592

1 mM, 95% inhibition

up to 1 mM complete inhibition

977, 978, 979

inhibits activation by Mn2+

1280

6.3.3.3

1 mM, 100% inhibition

1333

6.3.4.4

reversed by addition of DTT

cytochrome c oxidase activities of strains AP19-3 and ATCC 23270 are completely inhibited by 0.001 mM and 0.005 mM. Strain MON-1 is inhibited 33% by 0.005 mM, and 70% by 0.010 mM

0.25 mM, 50% inhibition

684657

7.2.4.2

complete inactivation at 1 mM, buffer-dependend, interaction with alpha-subunit

4280

Metals and Ions (265 results)

strong inhibition

complete inhibition at 0.1 mM

inhibitory

induces the enzyme in cut roots treated with HgCl2

655032

1.1.99.29

25% inhibition of activity

activates

enhances enzyme activity

713387

1.4.1.2

increases NADH-GDH activity substantially, however, specific activity of the enzyme decreases at lower concentration of Hg, and increases to lesser extent at higher concentration of Hg

673970

1.5.1.7

complete inhibition at 1 mM

strong inhibition

increase of enzyme mRNA expression, via a transcriptional mechanism

activation of bilirubin UDP-glucuronyltransferase

10 mM, complete inhibition

673390

2.4.1.206

4% activity compared to Mn2+

638232

2.6.1.5

a dose of 1-3 mg/kg increases the basal activity of the enzyme and decreases its induction by dexamethasone

674229

2.7.1.81

requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+

642034

2.7.10.2

increases tyrosine phosphorylation in vivo, genistein decreases the activating effect

activates

significantly inhibits enzyme activity

704231

3.1.1.53

1 mM, block of essential SH-groups

activates

decreases activity

inhibitory effect

low concentrations stimulate the oxidized enzyme form, but not the reduced enzyme form many hundred-fold. Half-maximal stimulation at 0.2 femtoM: Hg2+ stimulates by binding to an enzyme thiol group, thereby stabilizing the oxidized enzyme in an active conformation

exposure of newborn rats to mercury increases the hepatic alanine aminotransferase activity by fold and glucose 6-phosphatase activity by 75%. Zinc pre-exposure prevents totally and partially these mercury alterations, respectively. In vitro, HgCl2 inhibits the serum and liver alanine aminotransferase by 22% and 54%, respectively, serum and liver lactate dehydrogenase by 53% and 64%, respectively, and liver and kidney glucose 6-phosphatase from 10- to 13-day-old rats by 53% and 41%, respectively

729542

3.1.30.1

maximal activity at 0.01-1 mM at pH 3.5-5.0

complete inhibition

deleterious for enzyme activity

701955

3.2.1.169

inhibits activity at 1 mM

708967

3.2.1.18

complete inhibition at 1 mM

110.4% relative activity at 2 mM

704131

3.2.1.39

42% residual activity at 1 mM HgCl2

activates

2 mM, enzyme retains 25% of its activity

749983

3.2.1.78

454.6% activity at 5 mM

inhibitor

activates at 1 mM, complete inhibition at 5-10 mM

731160

3.4.24.72

about 150% activity at 2 mM

752343

3.5.1.2

106.59% activity at 1 mM

775436

3.5.2.2

at 2 mM 0% relative enzyme activity

669852

3.5.3.1

chelating loosely bound Mn2+ and replacing it with a variety of bivalent metal ions including Mg2+, Zn2+, Ni2+, Hg2+, Cu2+, Co2+, Ca2+ and Cd2+ retains its enzymatic activity

754624

3.5.4.3

complete inhibition at 1 mM

5 mM, 5.2fold activation

activates

1 mM, no activity is retained

moderate activity

1 mM, can replace Ca2+ in activation

666277

1R76, 3D-view

4.2.2.23

isoform Bo4416 shows 143% activity at 5 mM Hg2+

0.01 mM, stimulates

10 microM 100% inhibition, 1 microM 67% activity left

135294

4.6.1.19

ion inhibits DNA hydrolyzing activity

human ferrochelatase is capable of catalyzing the insertion of the inhibitory metal ion Hg2+ into a porphyrin macrocycle

705158

5.1.3.2

1 mM Hg2+ abolishes enzyme function entirely

702141

5.1.3.5

1 mM Hg2+ abolishes enzyme function entirely

inhibitory

inhibition

3D Structure of Enzyme-Ligand-Complex (PDB) (1367 results)

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (16 results)

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1.16.1.1

16 entries

K_M Value (22 results)

top print hide

1.16.1.1

22 entries

K_i Value (23 results)

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1.1.1.49

0.542

pH 6.0, 25°C

723747

1.14.18.1

0.15

immobilized enzyme, at pH 6.8 and 25°C

766437

1.2.98.1

0.0002

noncompetitive inhibition

0.00033

0.0005

pH 7.4, 37°C

659848

2.4.1.1

0.00038

pH 6.8, 25°C

736105

3.1.1.20

0.57

40°C

0.007

0.11

0.0446

pH 6, 50°C

0.05

0.55

0.0005

0.1

0.15

IC₅₀ Value (42 results)

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1.1.1.153

0.00009

IC50 0.00009 mM

286021

1.1.1.49

0.87

pH 6.0, 25°C

723747

1.14.18.1

0.03256

immobilized enzyme, at pH 6.8 and 25°C

766437

1.16.1.1

0.27

pH 7.4, 37°C

727979

1.2.1.12

6.2

at 25°C

0.17

IC50: 0.17 mM

657650

1.8.1.7

0.509

pH 8.0, 25°C

686233

2.3.1.6

0.0025

noncompetitive with respect to choline, IC50: 0.0004 mM, mixed type inhibition with respect to acetyl-CoA, IC50: 0.0025 mM, activity can be recovered using 2,3-dimercapto-propanol

659848

2.4.1.173

0.5

strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.5

672427

2.5.1.18

0.000000873

0.00000585

701942

2.7.7.1

0.017

IC50: 0.017 mM