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Ligand 5-formyltetrahydrofolate
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Basic Ligand Information
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate, (6S)-5-formyltetrahydrofolate, (S)-2-{4-[((S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydro-pteridin-6-ylmethyl)-amino]-benzoylamino}-pentanedioic acid, 5-formyl-5,6,7,8 tetrahydrofolate, 5-formyl-5,6,7,8-tetrahydrofolate, 5-formyl-5,6,7,8-tetrahydropteroyl-Glu, 5-formyl-5,6,7,8-tetrahydropteroylglutamate, 5-formyl-tetrahydrofolate, 5-formyltetrahydrofolic acid, Folinate, folinic acid, N5-formyltetrahydrofolate
Related pathways
BRENDA
MetaCyc
folate transformations I, folate transformations II (plants), folate transformations III (E. coli)
Show all BRENDA pathways known for 5-formyltetrahydrofolate
close all tables 
open all tablesRoles as Enzyme Ligand
In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)
top
EC NUMBER 
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + 5-formyltetrahydrofolate = ADP + 5,10-methenyltetrahydrofolate + phosphate
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ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
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Substrate in Enzyme-catalyzed Reactions (32 results)
EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5-formyltetrahydrofolate + L-glutamate = N-formyl-L-glutamate + tetrahydrofolate
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5-formyltetrahydrofolic acid + H2O = 5-formylpteroate + Glu
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ATP + (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate = ADP + phosphate + (6S)-5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
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ATP + 5-formyl-5,6,7,8-tetrahydrofolate + L-glutamate = ADP + 5-formyl-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
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ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate = ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
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ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate = ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
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ATP + 5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate = ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
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ATP + (6S)-5-formyltetrahydrofolate = ADP + 5,10-methenyltetrahydrofolate + phosphate
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araATP + 5-formyltetrahydrofolate = araADP + phosphate + 5,10-methylenetetrahydrofolate
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ATP + 5-formyltetrahydrofolate = ADP + 5,10-methenyltetrahydrofolate + phosphate
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ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
652430, 652260, 649293, 652326, 703027, 701907, 728751, 1320, 1328, 1322, 1321, 1331, 663298, 663363, 775630
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ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methylenetetrahydrofolate
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ATPgammaS + 5-formyltetrahydrofolate = ADPgammaS + phosphate + 5,10-methylenetetrahydrofolate
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CTP + 5-formyltetrahydrofolate = CDP + phosphate + 5,10-methylenetetrahydrofolate
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GTP + 5-formyltetrahydrofolate = GDP + phosphate + 5,10-methylenetetrahydrofolate
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UTP + 5-formyltetrahydrofolate = UDP + phosphate + 5,10-methylenetetrahydrofolate
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Product in Enzyme-catalyzed Reactions (4 results)
EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5-formyltetrahydrofolate heptaglutamate + 7 H2O = 5-formyltetrahydrofolate + 7 L-glutamate
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5-formyltetrahydrofolate hexaglutamate + 6 H2O = 5-formyltetrahydrofolate + 6 L-glutamate
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Enzyme Cofactor/Cosubstrate (1 result)
EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
Inhibitor in Enzyme-catalyzed Reactions (22 results)
EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant
3D Structure of Enzyme-Ligand-Complex (PDB) (212 results)
EC NUMBER
ENZYME 3D STRUCTURE
Enzyme Kinetic Parameters
kcat Value (Turnover Number) (20 results)
EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.046
-
mutant enzyme R7A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
0.1
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mutant enzyme K3D/D151K, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
0.17
-
mutant enzyme K3A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
0.35
-
mutant enzyme R125A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
0.77
-
mutant enzyme D81A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
0.79
-
wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C
0.8
-
mutant enzyme W153A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
1
-
wild type enzyme, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
1.3
-
mutant enzyme D151A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
1.3
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mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C
1.7
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mutant enzyme K120A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
KM Value (36 results)
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0025
-
wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C
0.0071
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mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C
1
4
mutant enzyme D151A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
2.5
-
wild type enzyme, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
5.8
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mutant enzyme D81A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
7.1
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mutant enzyme K120A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
21
-
mutant enzyme W153A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
31
-
mutant enzyme R7A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
51
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mutant enzyme K3A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
75
-
mutant enzyme R125A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
130
-
mutant enzyme K3D/D151K, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C
Ki Value (1 result)
References & Links
Literature References (53)
Links to other databases for 5-formyltetrahydrofolate
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Release 2026.1 (March 2026)
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