Ligand dCTP

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Basic Ligand Information

Molecular Structure
Picture of dCTP (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C9H16N3O13P3
dCTP
RGWHQCVHVJXOKC-SHYZEUOFSA-N
Synonyms:
2'-dCTP, 2'-deoxycytidine-5'-triphosphate, 2'-deoxycytidine 5'-triphosphate, 2'-deoxycytidine triphosphate, 5'-dCTP, deoxy-5'-CTP, deoxycytidine 5'-triphosphate


Show all pahtways known for Show all BRENDA pathways known for dCTP

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
dCTP + phosphatidate = diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
dCTP + H2O = dUTP + NH3
show the reaction diagram
dCTP + H2O = dUTP + NH3
show the reaction diagram
dCTP + H2O = dUMP + diphosphate + NH3
show the reaction diagram
dCTP + H2O = dCMP + diphosphate
show the reaction diagram
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dCTP + H2O = dCDP + phosphate
show the reaction diagram
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dCTP + H2O = dCMP + diphosphate
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
dCDP + (phosphate)n+1 = dCTP + (phosphate)n
show the reaction diagram
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ATP + dCDP = ADP + dCTP
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (270 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5,10-methylenetetrahydrofolate + dCTP = dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
show the reaction diagram
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dCTP + dolichol = dCDP + dolichyl phosphate
show the reaction diagram
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dCTP + deoxyguanosine = dCDP + dGMP
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dCTP + D-ribose = dCDP + D-ribose 5-phosphate
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dCTP + 1,2-diacyl-sn-glycerol = dCDP + 1,2-diacyl-sn-glycerol 3-phosphate
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dCTP + NAD+ = dCDP + NADP+
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pantothenate + dCTP = 4'-phosphopantothenate + dCDP
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dCTP + mevalonate = dCDP + phosphomevalonate
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dCTP + 4-methyl-5-(2-hydroxyethyl)thiazole = dCDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
show the reaction diagram
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dCTP + D-galactose = dCDP + alpha-D-galactose 1-phosphate
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dCTP + deoxyadenosine = dCDP + dAMP
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2'-deoxycytidine 5'-triphosphate + 3-phospho-D-glycerate = 2'-deoxycytidine 5'-diphosphate + 1,3-diphosphoglycerate
show the reaction diagram
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dCTP + 5-methyldeoxycytidine 5'-phosphate = dCDP + 5-methyldeoxycytidine diphosphate
show the reaction diagram
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dCTP + CMP = dCDP + CDP
show the reaction diagram
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dCTP + AMP = dCDP + ADP
show the reaction diagram
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dCTP + dTMP = dCDP + dTDP
show the reaction diagram
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dCTP + UMP = dCDP + UDP
show the reaction diagram
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dCTP + ethanolamine phosphate = diphosphate + dCDP-ethanolamine
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dCTP + alpha-D-glucose 1-phosphate = dCDP-glucose + diphosphate
show the reaction diagram
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dCTP + 3-deoxy-D-manno-octulosonate = diphosphate + dCMP-3-deoxy-D-manno-octulosonate
show the reaction diagram
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dCTP + glycerol 3-phosphate = diphosphate + dCDPglycerol
show the reaction diagram
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dCTP + phosphatidate = diphosphate + dCDPdiacylglycerol
show the reaction diagram
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dCTP + tobramycin = diphosphate + 2''-adenylyltobramycin
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dCTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate = diphosphate + dCDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
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dCTP + alpha-D-glucose 1-phosphate = diphosphate + dCDP-alpha-D-glucose
show the reaction diagram
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dCTP + H2O = ?
show the reaction diagram
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5'-dCTP + H2O = ?
show the reaction diagram
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glucose + dCTP = glucose 6-phosphate + dCDP
show the reaction diagram
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dCTP + H2O = dUTP + NH3
show the reaction diagram
dCTP + H2O = dUMP + diphosphate + NH3
show the reaction diagram
dCTP + H2O = dUTP + NH3
show the reaction diagram
-
dCTP + H2O = dCMP + diphosphate
show the reaction diagram
-
dCTP + H2O = dCDP + phosphate
show the reaction diagram
-
dCTP + H2O = dCMP + diphosphate
show the reaction diagram
-
dCTP + H2O = dCDP + phosphate
show the reaction diagram
-
dCTP + H2O = dCDP + phosphate
show the reaction diagram
-
dCTP + H2O = dCMP + diphosphate
show the reaction diagram
-
dCTP + H2O = dCDP + phosphate
show the reaction diagram
-
dCTP + H2O + closed Cl- channel = dCDP + phosphate + open Cl- channel
show the reaction diagram
ATP + dCTP = P1-(5'-adenosyl),P4-[5'-(2'-deoxycytidyl)]tetraphosphate + diphosphate
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (13 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
CTP + formate = dCTP + CO2 + H2O
show the reaction diagram
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CTP + reduced thioredoxin = 2'-dCTP + thioredoxin disulfide + H2O
show the reaction diagram
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dCDP + phosphoenolpyruvate = dCTP + pyruvate
show the reaction diagram
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dCDP + phosphoramide = dCTP + NH3
show the reaction diagram
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dCDP + (phosphate)n+1 = dCTP + (phosphate)n
show the reaction diagram
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ATP + dCMP = ADP + dCTP
show the reaction diagram
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-
dCTP + H2O = dCTP + phosphate
show the reaction diagram
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-
ATP + dUTP + NH4+ = ADP + phosphate + dCTP
show the reaction diagram
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Enzyme Cofactor/Cosubstrate (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE

Activator in Enzyme-catalyzed Reactions (41 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
less effective than ATP
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allosteric activator, less potent than dGTP and dATP
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at least 2fold enhancement of enzyme activity, maximal at 20 microM, similar results with all four deoxynucleoside triphosphates. Apparent KM for dCTP on a single-nucleotide gapped DNA as substrate: 0.2 microM. It is suggested that the mechanism of stimulation may involve either Pol beta protein conformational changes upon nucleoside triphosphate binding or altered protein-DNA interactions accomanying dCMP incorporation
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low activating effect
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Inhibitor in Enzyme-catalyzed Reactions (55 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
20% inhibition
-
allosteric inhibition
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competitive versus ATP, feedback inhibition
both a substrate and competitive inhibitor
-
80% inhibition at 0.3 mM
-
inhibits reaction with ATP and UMP, CMP or dCMP
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inhibits SurE, YfbR, not YjjG, competitive
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0.2 mM, 19% residual activity
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allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
competitive inhibition of hydrolysis of dCDP
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competitive to ATP
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competitive inhibitor with dTTP as substrate, Ki: 0.25 mM
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1 mM, inhibition is more pronounced at 37C compared to 70C
-
very weak inhibitor
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3D Structure of Enzyme-Ligand-Complex (PDB) (120 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (76 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.5
-
-
4
-
-
1.22
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
5.7
-
pH 9.5, 42C
0.022
-
37C
0.99
-
pH 8.0, 70C
14.3
-
pH 6.6, 25C

KM Value (101 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0091
-
pH 7.2, 37C
0.839
-
-
0.82
-
reaction with CMP, liver enzyme
0.02
-
pH 7.5
10.5
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
0.071
-
37C, primer (dA)12-18
0.34
-
pH 8.0, 37C
0.084
-
-
0.64
-
pH 9.5, 42C
17.6
-
60C, pH 7.5
0.071
-
37C
0.091
-
-
1.7
-
pH 8.0, 70C
0.22
-
pH 6.6, 25C

Ki Value (16 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
TABLE ID
0.75
-
pH 7, 37C
16200
3
-
pH 7.5, 25C, inhibition of ATPase activity
34464
0.25
-
competitive inhibitor with dTTP as substrate
34688

IC50 Value (1 result)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.04
-
IC50: 0.04 mM

References & Links

Links to other databases for dCTP

ChEBI
PubChem
ChEBI
PubChem