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Ligand 3-alpha-mycarosylerythronolide B

Basic Ligand Information

Molecular Structure

Show all BRENDA pathways known for 3-alpha-mycarosylerythronolide B

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

LITERATURE

REACTION DIAGRAM

PROVEN IN VIVO REACTION

ENZYME 3D STRUCTURE

2.4.1.278

715246

dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B = dTDP + erythromycin D

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In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

LITERATURE

REACTION DIAGRAM

PROVEN IN VIVO REACTION

ENZYME 3D STRUCTURE

2.4.1.328

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dTDP-beta-L-mycarose + erythronolide B = dTDP + 3-alpha-L-mycarosylerythronolide B

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Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Substrate (3 entries)

EC NUMBER

LITERATURE

REACTION DIAGRAM

REACTION

ENZYME 3D STRUCTURE

2.4.1.278

715246, 715245

dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B = dTDP + erythromycin D

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2.4.1.278

715246

dTDP-D-mycaminose + 3-alpha-mycarosylerythronolide B = dTDP + erythromycin M

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2.4.1.278

735516

dTDP-D-desosamine + 3-alpha-L-mycarosylerythronolide B = dTDP + erythromycin D

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2.4.1.278

3 entries

Product in Enzyme-catalyzed Reactions (2 results)

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Show Product (2 entries)

EC NUMBER

LITERATURE

REACTION DIAGRAM

REACTION

ENZYME 3D STRUCTURE

2.4.1.277

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dTDP-beta-L-mycarose + erythronolide B = dTDP + 3-alpha-L-mycarosylerythronolide B

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2.4.1.328

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dTDP-beta-L-mycarose + erythronolide B = dTDP + 3-alpha-L-mycarosylerythronolide B

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Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (2 results)

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Show Turnover Number (2 entries)

COMMENTARY

EC NUMBER

LITERATURE

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an antibiotic glycosyl transferase

2.4.1.278

715245

1.7

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pH and temperature not specified in the publication, 0.05 mM TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose

2.4.1.278

715246

0.017

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K_M Value (1 result)

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Show KM Value (1 entry)

COMMENTARY

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

LITERATURE

pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose

2.4.1.278

0.05

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715246

References & Links

Literature References (3)

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Show Reference (3 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

715245

15303858

Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway

2004

Lee, H.Y.; Chung, H.S.; Hang, C.; Khosla, C.; Walsh, C.T.; Kahne, D.; Walker, S.

J. Am. Chem. Soc.

126

9924-9925

715246

16218575

In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides

2005

Yuan, Y.; Chung, H.S.; Leimkuhler, C.; Walsh, C.T.; Kahne, D.; Walker, S.

J. Am. Chem. Soc.

127

14128-14129

735516

26552796

In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea

2016

Wu, H.; Li, W.; Xin, C.; Zhang, C.; Wang, Y.; Ren, S.; Ren, M.; Zhao, W.; Yuan, L.; Xu, Z.; Yuan, H.; Geng, M.; Zhang, L.; Weaver, D.T.; Zhang, B.

Appl. Microbiol. Biotechnol.

100

2257-2266

Links to other databases for 3-alpha-mycarosylerythronolide B

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Show Links (1 entry)

ChEBI

PubChem

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