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BRENDA support

Ligand inosine

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Basic Ligand Information

Molecular Structure
Picture of inosine (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
Molfile
C10H12N4O5
inosine
UGQMRVRMYYASKQ-KQYNXXCUSA-N
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Synonyms:
9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]-1,9-dihydro-6H-purin-6-one, hypoxanthine ribose

Related pathways

Pathway Source
Pathways
BRENDA
purine metabolism
KEGG
ABC transporters, Nucleotide metabolism, Purine metabolism
MetaCyc
adenine and adenosine salvage III, adenine and adenosine salvage V, adenosine nucleotides degradation I, adenosine nucleotides degradation II, purine ribonucleosides degradation


Show all pahtways known for Show all BRENDA pathways known for inosine

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (14 results)

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EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
inosine + O2 = 9-riburonosylhypoxanthine + H2O
show the reaction diagram
-
inosine + 2 O2 + H2O = 9-riburonosylinosine + 2 H2O2
show the reaction diagram
-
inosine + phosphate = hypoxanthine + D-ribose 1-phosphate
show the reaction diagram
-
ATP + inosine = ADP + IMP
show the reaction diagram
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inosine + H2O = D-ribose + hypoxanthine
show the reaction diagram
inosine + H2O = hypoxanthine + D-ribose
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (8 results)

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EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
S-inosyl-L-homocysteine + H2O = inosine + L-homocysteine
show the reaction diagram
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S-inosyl-L-homocysteine + H2O = L-homocysteine + inosine
show the reaction diagram
-
-
adenosine + H2O = inosine + NH3
show the reaction diagram
adenosine + H2O = inosine + NH3
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (83 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
inosine + NAD+ + H2O = xanthosine + NADH
show the reaction diagram
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inosine + 2 O2 + H2O = 9-riburonosylinosine + 2 H2O2
show the reaction diagram
-
inosine + NAD+ + H2O = ? + NADH
show the reaction diagram
-
inosine + phosphate = hypoxanthine + D-ribose 1-phosphate
show the reaction diagram
-
inosine + phosphate = hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
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inosine + adenine = adenosine + hypoxanthine
show the reaction diagram
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ATP + inosine = ADP + inosine 5'-phosphate
show the reaction diagram
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ATP + inosine = ADP + inosine 5'-phosphate
show the reaction diagram
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phosphate + inosine = ?
show the reaction diagram
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3 inosine = ?
show the reaction diagram
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inosine + L-homocysteine = S-inosyl-L-homocysteine + H2O
show the reaction diagram
inosine + homocysteine = ?
show the reaction diagram
-
L-homocysteine + inosine = S-inosyl-L-homocysteine + H2O
show the reaction diagram
-
inosine + H2O = D-ribose + hypoxanthine
show the reaction diagram
-
inosine + H2O = hypoxanthine + D-ribose
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (45 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
guanosine + hypoxanthine = guanine + inosine
show the reaction diagram
-
-
IMP + H2O = inosine + phosphate
show the reaction diagram
-
-
5'-IMP + H2O = inosine + phosphate
show the reaction diagram
-
-
3'-IMP + H2O = inosine + phosphate
show the reaction diagram
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S-inosyl-L-homocysteine + H2O = inosine + L-homocysteine
show the reaction diagram
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S-inosyl-L-homocysteine + H2O = L-homocysteine + inosine
show the reaction diagram
-
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adenosine + H2O = inosine + NH3
show the reaction diagram
adenosine + H2O = inosine + NH3
show the reaction diagram
-
-
adenosine + H2O = inosine + NH3
show the reaction diagram
-
-
adenosine + H2O = inosine + NH3
show the reaction diagram
-
adenosine + H2O = inosine + NH3
show the reaction diagram
-
-

Activator in Enzyme-catalyzed Reactions (20 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
0.1 mM, increases activity 5fold
-

Inhibitor in Enzyme-catalyzed Reactions (50 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
very weak, non-competitive
-
1 mM, about 25% inhibition
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weak, competitive
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slight inhibition
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inhibits phosphorylation of guanosine
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concentrations above 0.1 mM lead to reduction in enzyme activity
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strong inhibition, enzymes N-I and N-II
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above 2 mM
competitive inhibitor of xanthosine hydrolysis
noncompetitive
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at 0.2 mM: 8.5% inhibition for form Lf, 10.5% inhibition for form R2, form R1 not inhibited
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at 6.25 mM, competitive inhibitor for uridine hydrolysis
competitive inhibition of deamination of adenosine 3'-phenylphosphonate
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inhibits independently of the A3 receptor
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3D Structure of Enzyme-Ligand-Complex (PDB) (43 results)

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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (168 results)

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EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.57
-
pH 7.4, 25°C
0.16
-
pH 8.0, 22°C
0.83
-
pH 7.0, 70°C
0.83
-
pH 7.5, 70°C, recombinant enzyme

KM Value (248 results)

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EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.044
-
-
0.072
-
-
0.005
-
pH 7.5, 25°C
0.09
-
pH 7.4, 25°C
0.64
-
pH 7.0, 70°C
0.64
-
pH 7.5, 70°C, recombinant enzyme
0.283
-
pH 7.2, 32°C

Ki Value (8 results)

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EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
2
-
pH 7.0, 70°C
3
-
pH 7.5, 37°C, wild-type enzyme, versus uridine
0.35
-
pH 7.5, 25°C

IC50 Value (2 results)

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EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE

References & Links

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Links to other databases for inosine

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EXTERNAL LINKS
ChEBI
PubChem
UniChem