Ligand K2HPO4

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Basic Ligand Information

Molecular Structure
Picture of K2HPO4 (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H3K2O4P
K2HPO4
ZPWVASYFFYYZEW-UHFFFAOYSA-L
Synonyms:
K2PO4-

Roles as Enzyme Ligand

Activator in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
enhancing effect on NAD-dependent 3-phospho-D-glycerate oxidation and 3-phosphohydroxypyruvate reduction but inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction
-
at 0.15 M three to fourfold stimulation of activity
-
0.05% (w/v) KH2PO4 stimulates the production of FFase to 18%
-
439% relative activity at 200 mM
-

Inhibitor in Enzyme-catalyzed Reactions (13 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
100 mM, 95% inhibition, 10 mM, 27% inhibition
-
inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction. It appears that the phosphate ion PO43- exerts its inhibitory effect by binding to the free enzyme and NADPH-enzyme complex
-
inhibition of glycine-CO2 exchange reaction
-
0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory
-
at 26 mM 50% inhibition if cytochrome c or ferricyanide is electron acceptor
-
above 10 mM
-
inhibits enzyme forms PC-1 and PC-2, PC-3 is stimulated (optimal concentration 75 mM)
-

Metals and Ions (13 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulates
-
deadenylylation assay with 10mM K2HPO4
-
IFO 3134, stimulation of enzyme production and activity
-
results in highest activity
-
relative activity: 439%
-
enzyme form PC-3 is stimulated, optimal concentration 75 mM, enzyme form PC-1 and enzyme form PC-2 are inhibited
-

Enzyme Kinetic Parameters

Ki Value (2 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

IC50 Value (2 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE

References & Links

Links to other databases for K2HPO4

ChEBI
PubChem
ChEBI
PubChem