Ligand 7,8-dihydrofolate

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Basic Ligand Information

Molecular Structure
Picture of 7,8-dihydrofolate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C19H21N7O6
7,8-dihydrofolate
OZRNSSUDZOLUSN-LBPRGKRZSA-N
Synonyms:
7,8-dihydropteroyl-Glu, 7,8-dihydropteroylglutamate, dihydrofolate, dihydrofolate monoglutamate, dihydrofolic acid, dihydropteroyl-Glu, dihydropteroyl-L-Glu, dihydropteroylglutamate, N-(4-{[(2-amino-4-oxo-3,4,7,8-tetrahydropteridin-6-yl)methyl]amino}benzoyl)-L-glutamic acid


Show all pahtways known for Show all BRENDA pathways known for 7,8-dihydrofolate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (30 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE

In Vivo Product in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
folate + NADPH + H+ = 7,8-dihydrofolate + tetrahydrofolate + NADP+
-
-
show the reaction diagram
5,10-methylenetetrahydrofolate + dCMP = dihydrofolate + deoxy-5-methylcytidylate
-
-
show the reaction diagram
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
show the reaction diagram
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoylglutamate = diphosphate + dihydrofolate
-
-
show the reaction diagram
ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate
-

Substrate in Enzyme-catalyzed Reactions (69 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
Dihydrofolate + NADPH = 5,6,7,8-Tetrahydrofolate + NADP+
-
show the reaction diagram
dihydrofolate + NADPH = tetrahydrofolate + NADP+
-
show the reaction diagram
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
-
show the reaction diagram
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
-
show the reaction diagram
dihydrofolic acid + H2O = dihydropteroic acid + Glu
-
show the reaction diagram
ATP + formate + dihydrofolate = ADP + phosphate + 10-formyldihydrofolate
-

Product in Enzyme-catalyzed Reactions (77 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
folate + NADPH + H+ = 7,8-dihydrofolate + tetrahydrofolate + NADP+
-
show the reaction diagram
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoylglutamate = diphosphate + dihydrofolate
-

Activator in Enzyme-catalyzed Reactions (1 result)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
activates
-

Inhibitor in Enzyme-catalyzed Reactions (47 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
competitive with respect to 5,10-methylenetetrahydrofolate and uncompetitive with respect to NADPH
-
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition above 0.005 mM
-
substrate inhibition
-
competitive with respect to methylenetetrahydrofolate
-
competitive inhibitor with respect to tetrahydrofolate
-
slight
-
noncompetitive with respect to dihydropteroate, competitive with respect to L-Glu
-
5,6,7,8-tetrahydropteroyl-Glu2 formation
-
treatment of cells with trimethoprim leads to inhibition due to accumulation of dihydrofolate through the inhibition of dihydrofolate reductase. Therefore falling dihydrofolate reductase activity leads to falling folylpolyglutamate synthase activity in a domino-like cascade
-
weak
-

3D Structure of Enzyme-Ligand-Complex (PDB) (130 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (204 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
+ NADPH
4
-
25°C, pH 7.4
123
-
above, pH 6.0, 25°C recombinant His-tagged enzyme
1.4
-
altered enzyme
0.243
-
at pH 7.0 and 10°C
8.2
-
at pH 7.0 and 15°C
10.8
-
at pH 7.0 and 20°C
14.8
-
at pH 7.0 and 25°C
18.7
-
at pH 7.0 and 30°C
25.3
-
at pH 7.0 and 35°C
32.5
-
at pH 7.0 and 40°C
39.6
-
at pH 7.0 and 45°C
28.8
-
at pH 7.0 and 5°C
5.9
-
deletion mutant DELTAAla145, at pH 7.0 and 25°C
24
-
deletion mutant DELTAArg52, at pH 7.0 and 25°C
96.3
-
deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C
31.9
-
deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C
0.37
-
deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C
23.1
-
deletion mutant DELTAGly121, at pH 7.0 and 25°C
1.7
-
deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C
0.22
-
deletion mutant DELTAGly67, at pH 7.0 and 25°C
16.5
-
deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C
41.1
-
deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C
0.38
-
double mutant L16M/L20M from plasmid
25.2
-
form I
100
-
isozyme I
3
-
mutant C85A/C152S, 15°C, pH 7.0
4.9
-
mutant carrying the K32M mutation in gene copies 1 and 3 with the Q67H mutation in all gene copies, pH 7.0, 30°C
0.49
-
mutant circular ANLYF-G6, 15°C, pH 7.0
43.7
-
mutant circular ANLYF-G7, 15°C, pH 7.0
38.7
-
mutant circular ANLYF-G8, 15°C, pH 7.0
34.8
-
mutant enzyme F96I, at pH 7.0 and 25°C
4.8
-
mutant enzyme R44A, at 15°C, pH not specified in the publication
3.05
-
mutant enzyme R44C, at 15°C, pH not specified in the publication
2.43
-
mutant enzyme R44D, at 15°C, pH not specified in the publication
0.69
-
mutant enzyme R44E, at 15°C, pH not specified in the publication
0.89
-
mutant enzyme R44F, at 15°C, pH not specified in the publication
1.39
-
mutant enzyme R44G, at 15°C, pH not specified in the publication
1.78
-
mutant enzyme R44H, at 15°C, pH not specified in the publication
1.59
-
mutant enzyme R44I, at 15°C, pH not specified in the publication
1.15
-
mutant enzyme R44K, at 15°C, pH not specified in the publication
5.7
-
mutant enzyme R44L, at 15°C, pH not specified in the publication
0.98
-
mutant enzyme R44M, at 15°C, pH not specified in the publication
3.8
-
mutant enzyme R44N, at 15°C, pH not specified in the publication
3.3
-
mutant enzyme R44P, at 15°C, pH not specified in the publication
0.3
-
mutant enzyme R44Q, at 15°C, pH not specified in the publication
1.9
-
mutant enzyme R44S, at 15°C, pH not specified in the publication
5.8
-
mutant enzyme R44T, at 15°C, pH not specified in the publication
2
-
mutant enzyme R44V, at 15°C, pH not specified in the publication
1.31
-
mutant enzyme R44W, at 15°C, pH not specified in the publication
2.41
-
mutant enzyme R44Y, at 15°C, pH not specified in the publication
0.89
-
mutant enzyme T86N, in 50 mM TES, pH 7.0, at 37°C
3.75
-
mutant enzyme T86S, in 50 mM TES, pH 7.0, at 37°C
4.54
-
mutant enzyme Y102F, at pH 7.0 and 25°C
1.7
-
mutant F31A/F34V/Q35H, pH 7.6, 23°C
1.1
-
mutant F31G/Q35N, pH 7.6, 23°C
4.3
-
mutant F31P, pH 7.6, 23°C
6.4
-
mutant F31P/Q35E, pH 7.6, 23°C
1.7
-
mutant F31P/Q35H, pH 7.6, 23°C
1.7
-
mutant F31R/F34A/Q35N, pH 7.6, 23°C
0.5
-
mutant F31R/F34T/Q35R, pH 7.6, 23°C
1
-
mutant F31R/F34T/Q35S, pH 7.6, 23°C
1
-
mutant F31R/Q35E, pH 7.6, 23°C
1.3
-
mutant F31S/Q35E, pH 7.6, 23°C
9.4
-
mutant H26Y/Q60K/A77V/V78A/Q81H/Q91S/L100V/E133A/A149T, pH 7.0, 25°C
3.8
-
mutant I51N/C59R/N108S/I164L, pH 7.0
0.2
-
mutant isoform that carries the K32M mutation in gene copies 1 and 3 and the Q67H mutation in gene copies 2 and 4, pH 7.0, 30°C
1.7
-
mutant L20M from plasmid
24.6
-
mutant linear ANLYF-G6, 15°C, pH 7.0
36.7
-
mutant linear ANLYF-G7, 15°C, pH 7.0
41.7
-
mutant linear ANLYF-G8, 15°C, pH 7.0
40.8
-
mutant M1A/C85A/C152S, 15°C, pH 7.0
4.6
-
mutant M1A/M16F/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
25.8
-
mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S, 15°C, pH 7.0
40.5
-
mutant M1A/M16N/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
40.5
-
mutant M1A/M16S/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
16.2
-
mutant M1P/C85A/C152S, 15°C, pH 7.0
4.8
-
mutant M1S/C85A/C152S, 15°C, pH 7.0
5.3
-
mutant M42A, pH 7.0, 25°C
14
-
mutant M42C, pH 7.0, 25°C
30.4
-
mutant M42E, pH 7.0, 25°C
15.9
-
mutant M42G, pH 7.0, 25°C
13
-
mutant M42H, pH 7.0, 25°C
26.6
-
mutant M42I, pH 7.0, 25°C
12.4
-
mutant M42L, pH 7.0, 25°C
18.9
-
mutant M42P, pH 7.0, 25°C
24
-
mutant M42Q, pH 7.0, 25°C
24.3
-
mutant M42S, pH 7.0, 25°C
22.6
-
mutant M42T, pH 7.0, 25°C
18.7
-
mutant M42V, pH 7.0, 25°C
15.7
-
mutant M42W, pH 7.0, 25°C
mutant M42Y, pH 7.0, 25°C
45.5
-
mutant N51I/C59R/N108S/I164L, pH 7.0
1.5
-
mutant N51I/C59R/N108T/I164L, pH 7.0
1.9
-
mutant N51I/C59R/S108N, pH 8.0, 25°C
1.23
-
mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C
1.42
-
mutant N51I/C59R/S108N/I164L, pH 7.0
72
-
mutant N51I/C59R/S108N/I164L/D187A, pH 7.0
13.6
-
mutant N51I/C59R/S108N/I164L/I150V/N182I/N201D, pH 7.0
11.4
-
mutant N51I/C59R/S108N/I164L/K96N, pH 7.0
28.3
-
mutant R53M enzyme
7.6
-
mutant type
15
-
mutant V115A, pH 7.6, 23°C
2.8
-
mutant V115C, pH 7.6, 23°C
0.65
-
native enzyme
7
-
pH 6.0
8.63
-
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.05
-
pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
1.39
-
pH 7, 20°C, mutant enzyme V11D
0.003
-
pH 7, 20°C, wild-type enzyme
pH 7.0, 25°C, under atmospheric pressure
pH 7.0, 30°C, mutant D31A
0.86
-
pH 7.0, 30°C, mutant D31E
3.58
-
pH 7.0, 30°C, mutant D31L
0.025
-
pH 7.0, 30°C, mutant D31N
5.03
-
pH 7.0, 30°C, mutant D31Q
1.5
-
pH 7.0, 30°C, mutant L32A
16.2
-
pH 7.0, 30°C, mutant L32D
7.2
-
pH 7.0, 30°C, mutant L32F
21
-
pH 7.0, 30°C, mutant V76A
18.7
-
pH 7.0, 30°C, wild-type
20.5
-
pH 7.4
5
-
pH 7.4, 37°C, mutant K37Q
3
6
pH 7.4, 37°C, mutant N64S
47
-
pH 7.4, 37°C, mutant Q35K
48
-
pH 7.4, 37°C, mutant Q35K/N64F
43
-
pH 7.4, 37°C, mutant Q35S
35
-
pH 7.4, 37°C, mutant Q35S/N64S
27
-
pH 7.4, 37°C, wild-type enzyme
pH 7.4, 37°C, wild-type enzyme and mutant N64F
40
-
pH 7.5, 25°C, cosubstrate: NADPH
51
-
pH 7.5, temperature not specified in the publication
22.8
-
pH 7.8, 25°C, recombinant enzyme
130
-
recombinant bifunctional enzyme
5
-
recombinant double mutant L30K/A31K
9.5
-
recombinant enzyme
recombinant mutant A31K
4.97
-
recombinant mutant F69S, pH 7.4, 37°C
204
-
recombinant mutant K37Q, pH 7.4, 37°C
38
-
recombinant mutant K37Q/F69N, pH 7.4, 37°C
223
-
recombinant mutant K37Q/F69S, pH 7.4, 37°C
158
-
recombinant mutant K37S, pH 7.4, 37°C
45
-
recombinant mutant K37S/F69N, pH 7.4, 37°C
190
-
recombinant mutant K37S/F69S, pH 7.4, 37°C
157
-
recombinant mutant L30K
100
-
recombinant mutant N64F, pH 7.4, 37°C
2
8
recombinant mutant N64S, pH 7.4, 37°C
3
6
recombinant mutant Q35K, pH 7.4, 37°C
39
-
recombinant mutant Q35K/N64F, pH 7.4, 37°C
43
-
recombinant mutant Q35K/N64S, pH 7.4, 37°C
3
6
recombinant mutant Q35S, pH 7.4, 37°C
24
-
recombinant mutant Q35S/N64F, pH 7.4, 37°C
21
-
recombinant mutant Q35S/N64S, pH 7.4, 37°C
23
-
recombinant mutant S108A
21
-
recombinant mutant S108C
12
-
recombinant mutant S108G
23
-
recombinant mutant S108N
92
-
recombinant mutant S108Q
37
-
recombinant mutant S108T
36
-
recombinant mutant S37K/S69F, pH 7.4, 37°C
57
-
recombinant mutant S37K/S69N, pH 7.4, 37°C
78
-
recombinant mutant S37Q, pH 7.4, 37°C
51
-
recombinant mutant S37Q/S69F, pH 7.4, 37°C
26
-
recombinant mutant S37Q/S69N, pH 7.4, 37°C
95
-
recombinant mutant S69F, pH 7.4, 37°C
83
-
recombinant mutant S69N, pH 7.4, 37°C
47
-
recombinant wild-type enzyme
recombinant wild-type enzyme, pH 7.4, 37°C
triple mutant L16M/L20M/L42M from plasmid
52.7
-
wild type enzyme, at 15°C, pH not specified in the publication
5.4
-
wild type enzyme, at pH 7.0 and 25°C
wild type enzyme, in 50 mM TES, pH 7.0, at 37°C
3.11
-
wild-type
133
-
wild-type enzyme
wild-type enzyme from plasmid
13.7
-
wild-type, 15°C, pH 7.0
5.3
-
wild-type, pH 7.0, 25°C
wild-type, pH 7.6, 23°C
10
-
wild-type, pH 8.0, 25°C
1.24
-
with acetylpyridine adenine nucleotide
2
-
with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5
1.6
-
with NADPH
18
-
with NAPH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5
2.3
-
-
0.84
-

KM Value (242 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
25°C, pH 7.0, pressure 0.1 MPa
0.0178
-
25°C, pH 7.0, pressure 200 MPa
0.0195
-
25°C, pH 7.4
0.0018
-
25°C, recombinant detagged enzyme
0.0047
-
25°C, recombinant GST-tagged enzyme
0.0016
-
above, pH 6.0, 25°C recombinant His-tagged enzyme
0.0147
-
altered enzyme
0.01
-
at pH 7.0 and 22°C
0.03825
-
deletion mutant DELTAAla145, at pH 7.0 and 25°C
0.0008
-
deletion mutant DELTAArg52, at pH 7.0 and 25°C
0.1161
-
deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C
0.0777
-
deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C
0.0512
-
deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C
0.0502
-
deletion mutant DELTAGly121, at pH 7.0 and 25°C
0.0108
-
deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C
0.0073
-
deletion mutant DELTAGly67, at pH 7.0 and 25°C
0.0051
-
deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C
0.0048
-
deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C
0.0056
-
double mutant L16M/L20M from plasmid
0.00167
-
His-tagged enzyme, pH 7.5, 37°C
0.0029
-
His-tagged human-derived enzyme, pH 7.65
0.002
-
His-tagged recombinant enzyme, pH 7.5, 37°C
0.0029
-
lowest Km value in 3.0 M KCl, recombinant wild-type enzyme
0.08
-
mutant C85A/C152S, 15°C, pH 7.0
0.0012
-
mutant carrying the K32M mutation in gene copies 1 and 3 with the Q67H mutation in all gene copies, pH 7.0, 30°C
0.014
-
mutant circular ANLYF-G6, 15°C, pH 7.0
0.0115
-
mutant circular ANLYF-G7, 15°C, pH 7.0
0.013
-
mutant circular ANLYF-G8, 15°C, pH 7.0
0.014
-
mutant enzyme
0.097
-
mutant enzyme F96I, at pH 7.0 and 25°C
0.2301
-
mutant enzyme R44A, at 15°C, pH not specified in the publication
0.0016
-
mutant enzyme R44C, at 15°C, pH not specified in the publication
0.0011
-
mutant enzyme R44D, at 15°C, pH not specified in the publication
0.003
-
mutant enzyme R44E, at 15°C, pH not specified in the publication
0.0019
-
mutant enzyme R44F, at 15°C, pH not specified in the publication
0.0027
-
mutant enzyme R44G, at 15°C, pH not specified in the publication
0.0015
-
mutant enzyme R44H, at 15°C, pH not specified in the publication
0.0007
-
mutant enzyme R44I, at 15°C, pH not specified in the publication
0.0021
-
mutant enzyme R44K, at 15°C, pH not specified in the publication
0.0025
-
mutant enzyme R44L, at 15°C, pH not specified in the publication
0.0014
-
mutant enzyme R44M, at 15°C, pH not specified in the publication
0.0023
-
mutant enzyme R44N, at 15°C, pH not specified in the publication
0.0025
-
mutant enzyme R44P, at 15°C, pH not specified in the publication
0.0029
-
mutant enzyme R44Q, at 15°C, pH not specified in the publication
0.0024
-
mutant enzyme R44S, at 15°C, pH not specified in the publication
0.0031
-
mutant enzyme R44T, at 15°C, pH not specified in the publication
0.0025
-
mutant enzyme R44V, at 15°C, pH not specified in the publication
0.0008
-
mutant enzyme R44W, at 15°C, pH not specified in the publication
0.0037
-
mutant enzyme R44Y, at 15°C, pH not specified in the publication
0.001
-
mutant enzyme T86N, in 50 mM TES, pH 7.0, at 37°C
0.03051
-
mutant enzyme T86S, in 50 mM TES, pH 7.0, at 37°C
0.01625
-
mutant enzyme Y102F, at pH 7.0 and 25°C
0.01444
-
mutant F31A/F34V/Q35H, pH 7.6, 23°C
0.0043
-
mutant F31G/Q35N, pH 7.6, 23°C
0.0017
-
mutant F31P, pH 7.6, 23°C
0.0015
-
mutant F31P/Q35E, pH 7.6, 23°C
0.0027
-
mutant F31P/Q35H, pH 7.6, 23°C
0.0021
-
mutant F31R/F34A/Q35N, pH 7.6, 23°C
0.0022
-
mutant F31R/F34T/Q35R, pH 7.6, 23°C
0.002
-
mutant F31R/F34T/Q35S, pH 7.6, 23°C
0.0017
-
mutant F31R/Q35E, pH 7.6, 23°C
0.00069
-
mutant F31S/Q35E, pH 7.6, 23°C
0.0033
-
mutant H26Y/Q60K/A77V/V78A/Q81H/Q91S/L100V/E133A/A149T, pH 7.0, 25°C, value below
0.00063
-
mutant I51N/C59R/N108S/I164L, pH 7.0
0.0083
-
mutant isoform that carries the K32M mutation in gene copies 1 and 3 and the Q67H mutation in gene copies 2 and 4, pH 7.0, 30°C
0.0096
-
mutant K27E, pH 5.0, 25°C
0.0053
-
mutant L16M from plasmid
0.00222
-
mutant L20M from plasmid
0.00289
-
mutant L30Q, pH 8.5, 25°C
0.00592
-
mutant L42M from plasmid
0.00171
-
mutant L92M from plasmid
0.00123
-
mutant linear ANLYF-G6, 15°C, pH 7.0
0.0062
-
mutant linear ANLYF-G8, 15°C, pH 7.0
0.0086
-
mutant M1A/C85A/C152S, 15°C, pH 7.0
0.0013
-
mutant M1A/M16F/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
0.003
-
mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S, 15°C, pH 7.0
0.0083
-
mutant M1A/M16N/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
0.0083
-
mutant M1A/M16S/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
0.0038
-
mutant M1P/C85A/C152S, 15°C, pH 7.0
0.0012
-
mutant M1S/C85A/C152S, 15°C, pH 7.0
0.0025
-
mutant M42A, pH 7.0, 25°C
0.0013
-
mutant M42C, pH 7.0, 25°C
0.00194
-
mutant M42E, pH 7.0, 25°C
0.00597
-
mutant M42G, pH 7.0, 25°C
0.00118
-
mutant M42H, pH 7.0, 25°C
0.00193
-
mutant M42I, pH 7.0, 25°C
0.00061
-
mutant M42L, pH 7.0, 25°C
0.00112
-
mutant M42P, pH 7.0, 25°C
0.00083
-
mutant M42Q, pH 7.0, 25°C
0.00227
-
mutant M42S, pH 7.0, 25°C
0.00227
-
mutant M42T, pH 7.0, 25°C
0.00082
-
mutant M42V, pH 7.0, 25°C
0.00091
-
mutant M42W, pH 7.0, 25°C
0.045
-
mutant M42Y, pH 7.0, 25°C
0.00178
-
mutant N51I/C59R/N108S/I164L, pH 7.0
0.0052
-
mutant N51I/C59R/N108T/I164L, pH 7.0
0.005
-
mutant N51I/C59R/S108N, pH 8.0, 25°C
0.0209
-
mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C
0.0207
-
mutant N51I/C59R/S108N/I164L, pH 7.0
0.0133
-
mutant N51I/C59R/S108N/I164L/D187A, pH 7.0
0.0145
-
mutant N51I/C59R/S108N/I164L/I150V/N182I/N201D, pH 7.0
0.01465
-
mutant N51I/C59R/S108N/I164L/K96N, pH 7.0
0.0081
-
mutant Q134K, pH 8.5, 25°C
0.00004
-
mutant R53M enzyme
0.0016
-
mutant V115A, pH 7.6, 23°C
0.015
-
mutant V115C, pH 7.6, 23°C
0.007
-
mutant W48Y, 25°C, pH 7.0
0.0231
-
mutant W48Y/N188Y, 25°C, pH 7.0
0.0324
-
mutant Y35G/F37L, pH 5.0, 25°C
0.0049
-
mutant Y35L/F37R, pH 5.0, 25°C
0.0055
-
mutant Y35L/F37T, pH 5.0, 25°C
0.0086
-
mutant Y35Q/F37R, pH 5.0, 25°C
0.0076
-
native enzyme
0.0024
-
native rat-derived enzyme, pH 7.65
0.0106
-
pH 6.0
0.0095
-
pH 6.0, 28°C, recombinant enzyme
0.0556
-
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.0077
-
pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.0027
-
pH 7, 20°C, wild-type enzyme
0.0007
-
pH 7.0, 20°C
16.4
-
pH 7.0, 25°C, under atmospheric pressure
pH 7.0, 30°C
19.8
-
pH 7.0, 30°C, mutant D31A
0.037
-
pH 7.0, 30°C, mutant D31E
0.0019
-
pH 7.0, 30°C, mutant D31L
0.198
-
pH 7.0, 30°C, mutant D31N
0.0021
-
pH 7.0, 30°C, mutant D31Q
0.0023
-
pH 7.0, 30°C, mutant L32A
0.00096
-
pH 7.0, 30°C, mutant L32D
0.0051
-
pH 7.0, 30°C, mutant L32F
0.00068
-
pH 7.0, 30°C, mutant V76A
0.00078
-
pH 7.0, 30°C, wild-type
0.0007
-
pH 7.0, 5°C
21.7
-
pH 7.0, 60°C
0.0037
-
pH 7.3, 37°C
pH 7.4, 37°C
pH 7.4, 37°C, mutant K37Q
0.0085
-
pH 7.4, 37°C, mutant N64F
0.00081
-
pH 7.4, 37°C, mutant N64S
0.00018
-
pH 7.4, 37°C, mutant Q35K
0.00076
-
pH 7.4, 37°C, mutant Q35K/N64F
0.00049
-
pH 7.4, 37°C, mutant Q35S
0.0011
-
pH 7.4, 37°C, mutant Q35S/N64S
0.0016
-
pH 7.4, 37°C, wild-type enzyme
pH 7.5, 25°C
0.0019
-
pH 7.5, temperature not specified in the publication
0.0055
-
pH 7.8, 25°C, recombinant enzyme
0.0264
-
recombinant bifunctional enzyme
0.0006
-
recombinant enzyme
recombinant enzyme, pH 7.0, 0.1 M imidazole
0.0023
-
recombinant from E. coli
0.0027
-
recombinant mutant F69N, pH 7.4, 37°C
0.0066
-
recombinant mutant F69S, pH 7.4, 37°C
0.0071
-
recombinant mutant K37Q/F69N, pH 7.4, 37°C
0.0107
-
recombinant mutant K37Q/F69S, pH 7.4, 37°C
0.0185
-
recombinant mutant K37S, pH 7.4, 37°C
0.0101
-
recombinant mutant K37S/F69N, pH 7.4, 37°C
0.0097
-
recombinant mutant K37S/F69S, pH 7.4, 37°C
0.0116
-
recombinant mutant N64F, pH 7.4, 37°C
0.0008
-
recombinant mutant N64S, pH 7.4, 37°C
0.0009
-
recombinant mutant Q35K, pH 7.4, 37°C
0.0005
-
recombinant mutant Q35K/N64F, pH 7.4, 37°C
0.0004
-
recombinant mutant Q35K/N64S, pH 7.4, 37°C
0.0007
-
recombinant mutant Q35S, pH 7.4, 37°C
0.0007
-
recombinant mutant Q35S/N64F, pH 7.4, 37°C
0.0007
-
recombinant mutant Q35S/N64S, pH 7.4, 37°C
0.0004
-
recombinant mutant S108Q
0.05
-
recombinant mutant S37K/S69F, pH 7.4, 37°C
0.0032
-
recombinant mutant S37K/S69N, pH 7.4, 37°C
0.0024
-
recombinant mutant S37Q, pH 7.4, 37°C
0.0035
-
recombinant mutant S37Q/S69F, pH 7.4, 37°C
0.003
-
recombinant mutant S37Q/S69N, pH 7.4, 37°C
0.0038
-
recombinant mutant S69F, pH 7.4, 37°C
0.0048
-
recombinant mutant S69N, pH 7.4, 37°C
0.0034
-
recombinant mutants S108G, S108N and S108C
0.02
0.025
recombinant mutants S108T and S108A
0.014
-
recombinant rat-derived enzyme, pH 7.65
0.0056
-
recombinant wild-type enzyme
0.013
-
recombinant wild-type enzyme, pH 7.4, 37°C
strain PC 174
0.003
-
strain PC 178
0.004
-
triple mutant L16M/L20M/L42M from plasmid
0.00369
-
wild type enzyme, at 15°C, pH not specified in the publication
0.0016
-
wild type enzyme, at pH 7.0 and 25°C
wild type enzyme, in 50 mM TES, pH 7.0, at 37°C
0.00385
-
wild-type enzyme
wild-type enzyme from plasmid
0.00097
-
wild-type, 15°C, pH 7.0
0.0012
-
wild-type, 25°C, pH 7.0
0.0051
-
wild-type, pH 5.0, 25°C
0.0042
-
wild-type, pH 7.0, 25°C
wild-type, pH 7.5, 37°C
0.0013
-
wild-type, pH 7.6, 23°C
0.000075
-
wild-type, pH 8.0, 25°C
0.0444
-
wild-type, pH 8.5, 25°C
0.00119
-
with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5
0.0016
-
with NADPH
0.00015
-
recombinant enzyme, pH 4.7
0.0061
-
recombinant enzyme, pH 6.0
cosubstrate NADPH, pH 7.2, 25°C
0.04
-

Ki Value (7 results)

COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
-
0.0065
-
pH 6.7, 25°C
0.006
-
0.5 mM, noncompetitive inhibition, dUMP as variable substrate
0.00294
-
1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate
0.0014
-
1.0 mM, noncompetitive inhibition, dUMP as variable substrate
0.00303
-
5.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate
0.00178
-
-
0.021
-

References & Links

Links to other databases for 7,8-dihydrofolate

ChEBI
PubChem
-
PubChem