Any feedback?
No. of entries
Information on EC 7.3.2.1 - ABC-type phosphate transporter
for references in articles please use BRENDA:EC7.3.2.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 7 Translocases
7.3 Catalysing the translocation of inorganic anions and their chelates
7.3.2 Linked to the hydrolysis of a nucleoside triphosphate
7.3.2.1 ABC-type phosphate transporter
IUBMB Comments
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. A bacterial enzyme that interacts with an extracytoplasmic substrate binding protein and mediates the high affinity uptake of phosphate anions. Unlike P-type ATPases, it does not undergo phosphorylation during the transport process.
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
- 7.3.2.1
- pituitary
- prolactin
- phosphorus
-
pituitary-specific
- tubule
- calcification
- tsh
- mycorrhizal
-
reabsorption
- parathyroid
-
somatotrophs
-
homeodomain
- hyperphosphatemia
-
lactotrophs
-
co-transporter
-
hypophosphatemic
-
phosphaturic
-
phosphonoformic
-
thyrotropes
-
thyroid-stimulating
- arbuscules
-
p-aminohippurate
- probenecid
-
phosphate-induced
-
sodium-phosphate
-
amphotropic
- rickets
-
rhizophagus
-
non-mycorrhizal
-
corticotrophs
- irregularis
- hypopituitarism
-
phors
-
gh-secreting
- oxonate
- intraradices
-
gh-releasing
-
gonadotrophs
-
nherf1
-
na-dependent
-
para-aminohippurate
-
snell
-
hyperuricemic
-
hypercalciuria
-
hormone-producing
- gibbon
-
uricosuric
-
pi-induced
-
p-deficient
-
factor-23
Reaction Schemes
show+
+
=
+
+
+
+
+
phosphate-[phosphate-binding protein][side 1]
=
+
+
+
[phosphate-binding protein][side 1]
Synonyms
pit-1, phosphate transporter, npt2a, pho84, anion transporter 1, pit-2, pi transporter, slc20a1, glvr-1, pstscab, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ABC phosphate transporter
-
-
-
-
anion transporter 1
ANTR1
ATP phosphohydrolase (phosphate-importing)
-
-
-
-
CNH35_00300
EC 3.6.3.27
-
-
formerly
-
GlpT
GmPT1
GmPT2
high affinity phosphate transporter
high-affinity phosphate ABC-transporter
high-affinity phosphate transporter
high-affinity Pi transporter
HvPHT1;1
inorganic phosphate transporter
mTPT1
MtPT4
mycorrhiza-specific phosphate transporter
Na/Pi cotransporter
NptA
Phn
Pho84
Pho87
Pho90
phosphate specific transporter
-
-
-
-
phosphate transporter
phosphate transporter 1
PHOSPHATE TRANSPORTER 4;1
phosphate-specific transport system
-
-
-
-
phosphate-specific transporter
phosphate-transporting ATPase
-
-
-
-
PHT1
PHT1 transporter
Pht1:7
Pht1;4
Pht1;8 transporter
PHT1;9
Pht1;9 transporter
PHT4;1
Pi transporter
PIIN_08131
PiPT
Pit
PiT-1
PiT-2
Pit1
Pit2
Pst
PstA permease
PstABC
PstS
PstSCAB
PT1
PT2
PT4
PT5
PT8
PT9
SLC20A1
symbiosis-specific phosphate transporter
type III Pi transporter
additional information
Pst
-
-
-
-
additional information
-
the enzyme belongs to the Pst ABC-type permease family
additional information
-
the enzyme belongs to the PstB-like ABC-family of permeases
additional information
the enzyme belongs to the Pht1 family of plant phosphate transporters
additional information
the enzyme belongs to the Pht1 family of plant phosphate transporters
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + phosphate-[phosphate-binding protein][side 1] = ADP + phosphate + phosphate[side 2] + [phosphate-binding protein][side 1]
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric acid anhydride
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
MetaCyc
arsenate detoxification I
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (ABC-type, phosphate-importing)
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. A bacterial enzyme that interacts with an extracytoplasmic substrate binding protein and mediates the high affinity uptake of phosphate anions. Unlike P-type ATPases, it does not undergo phosphorylation during the transport process.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9000-83-3
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + H2O + phosphate-[phosphate-binding protein][side 1]
ADP + phosphate + phosphate[side 2] + [phosphate-binding protein][side 1]
ATP + H2O + tellurite-[phosphate-binding protein][side 1]
ADP + phosphate + tellurite[side 2] + [phosphate-binding protein][side 1]
Substrates: -
Products: -
Products: -
?
ATP + H2O + arsenate/out
ADP + phosphate + arsenate/in
Substrates: the enzyme has about 150fold lower affinity for arsenate compared to phosphate
Products: -
Products: -
?
ATP + H2O + arsenate/out
ADP + phosphate + arsenate/in
-
Substrates: the affinity of Pht1;3 for arsenate is much greater than for phosphate
Products: -
Products: -
?
ATP + H2O + phosphate-[phosphate-binding protein][side 1]
ADP + phosphate + phosphate[side 2] + [phosphate-binding protein][side 1]
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate-[phosphate-binding protein][side 1]
ADP + phosphate + phosphate[side 2] + [phosphate-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate-[phosphate-binding protein][side 1]
ADP + phosphate + phosphate[side 2] + [phosphate-binding protein][side 1]
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: ANTR1 functions as a Na+-dependent phosphate transporter
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: phosphate is the preferred substrate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the enzyme performs phosphate uptake energized by ATP hydrolysis
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: ATP-consuming import of phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: as phosphate/proton cotransport mechanism
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: as phosphate/proton cotransport mechanism
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: Pit-1 is required for elevated phosphate-induced osteogenic gene expression in human smooth muscle cells, role of the sodium-dependent phosphate cotransporter, Pit-1, in vascular smooth muscle cell calcification, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: proton-coupled phosphate transport
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the enzyme performs phosphate uptake energized by ATP hydrolysis
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: ATP-consuming import of phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: import of phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the enzyme performs phosphate uptake energized by ATP hydrolysis
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: conformational and structural changes upon nucleotide binding analysis, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: ATP-consuming import of phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: involved in promoting drug efflux in the CIPr clone
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: involved in phosphate transport
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: confers high levels of fluoroquinolone resistance in ciproflavin resistant colony CIPr
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the enzyme is involved in the phosphate uptake system in the alfalfa symbiont Sinorhizobium meliloti, PhoB has regulatory function, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: PstSCAB is specific for phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the Pst system affects autolysis and transformation, it is most likely involved in a signalling pathway regulating the activity of the major pneumococcal autolysin
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
CTP + H2O + phosphate/out
CDP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
CTP + H2O + phosphate/out
CDP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
GTP + H2O + phosphate/out
GDP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
GTP + H2O + phosphate/out
GDP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme plays an important role in phosphate acquisition and remobilization in plants, its activity is regulated by the phosphate transporter traffic facilitator 1, PHF1, enabling the endoplasmic reticulum exit of the enzyme, mechanism, phf1-deficient phenotypes, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme binds to phosphate transporter traffic facilitator 1, PHF1, a SEC12-related plant-specific protein
Products: -
Products: -
?
additional information
?
-
-
Substrates: the exceptional toxicity of arsenate, As(V), is derived from its close chemical similarity to phosphate, which allows the metalloid to be easily incorporated into plant cells through the high-affinity Pi transport system
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: ALIX protein regulates vacuolar degradation of enzyme variant PHT1;1
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) interacts with C-terminal end of sodium-dependent phosphate transporter 2a
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
Substrates: HvPHT1;1 shows preferential selectivity for phosphate and arsenate, but no transport of the other oxyanions SO42- and NO3-
Products: -
Products: -
?
additional information
?
-
-
Substrates: HvPHT1;1 shows preferential selectivity for phosphate and arsenate, but no transport of the other oxyanions SO42- and NO3-
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
Substrates: protein SLC25A3 transports both copper and phosphate
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: the high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the organism requires a functional phosphate import system for infection of hosts via the urinary tract, Pst negatively regulates biofilm formation and pathogenesis. The mutants show an altered proteome compared to the wild-type cells
Products: -
Products: -
?
additional information
?
-
-
Substrates: the high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the organism requires a functional phosphate import system for infection of hosts via the urinary tract, Pst negatively regulates biofilm formation and pathogenesis. The mutants show an altered proteome compared to the wild-type cells
Products: -
Products: -
?
additional information
?
-
-
Substrates: the phosphate transporter PiT-2 is involved in developmental regulation in renal tubules
Products: -
Products: -
?
additional information
?
-
-
Substrates: N-terminal SPX domain of Pho90 interacts physically with regulatory protein Spl2
Products: -
Products: -
?
additional information
?
-
-
Substrates: Spl2 interacts with the N-terminal SPX domain of Pho87 and Pho90
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: Spl2 interacts with the N-terminal SPX domain of Pho87 and Pho90
Products: -
Products: -
?
additional information
?
-
Substrates: PT4 is invlved in nutrient supply by mycorrhiza and mycorrhiza-specific phosphate transport in Solanaceae plants, regulation, overview, comparison to other Pht1 family phosphate transporters in mycorrhizal Pi transport
Products: -
Products: -
?
additional information
?
-
-
Substrates: PT4 is invlved in nutrient supply by mycorrhiza and mycorrhiza-specific phosphate transport in Solanaceae plants, regulation, overview, comparison to other Pht1 family phosphate transporters in mycorrhizal Pi transport
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
Substrates: PT4 is invlved in nutrient supply by mycorrhiza and mycorrhiza-specific phosphate transport in Solanaceae plants, regulation, overview, comparison to other Pht1 family phosphate transporters in mycorrhizal Pi transport
Products: -
Products: -
?
additional information
?
-
-
Substrates: PT4 is invlved in nutrient supply by mycorrhiza and mycorrhiza-specific phosphate transport in Solanaceae plants, regulation, overview, comparison to other Pht1 family phosphate transporters in mycorrhizal Pi transport
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: PstS is involved in penicillin resistance of Streptococcus pneumoniae
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + H2O + phosphate-[phosphate-binding protein][side 1]
ADP + phosphate + phosphate[side 2] + [phosphate-binding protein][side 1]
ATP + H2O + phosphate-[phosphate-binding protein][side 1]
ADP + phosphate + phosphate[side 2] + [phosphate-binding protein][side 1]
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate-[phosphate-binding protein][side 1]
ADP + phosphate + phosphate[side 2] + [phosphate-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: ANTR1 functions as a Na+-dependent phosphate transporter
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the enzyme performs phosphate uptake energized by ATP hydrolysis
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: ATP-consuming import of phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: as phosphate/proton cotransport mechanism
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: as phosphate/proton cotransport mechanism
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: Pit-1 is required for elevated phosphate-induced osteogenic gene expression in human smooth muscle cells, role of the sodium-dependent phosphate cotransporter, Pit-1, in vascular smooth muscle cell calcification, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: proton-coupled phosphate transport
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the enzyme performs phosphate uptake energized by ATP hydrolysis
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: ATP-consuming import of phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: import of phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the enzyme performs phosphate uptake energized by ATP hydrolysis
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: ATP-consuming import of phosphate
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: involved in promoting drug efflux in the CIPr clone
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: involved in phosphate transport
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: confers high levels of fluoroquinolone resistance in ciproflavin resistant colony CIPr
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the enzyme is involved in the phosphate uptake system in the alfalfa symbiont Sinorhizobium meliloti, PhoB has regulatory function, overview
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
-
Substrates: the Pst system affects autolysis and transformation, it is most likely involved in a signalling pathway regulating the activity of the major pneumococcal autolysin
Products: -
Products: -
?
ATP + H2O + phosphate/out
ADP + phosphate + phosphate/in
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme plays an important role in phosphate acquisition and remobilization in plants, its activity is regulated by the phosphate transporter traffic facilitator 1, PHF1, enabling the endoplasmic reticulum exit of the enzyme, mechanism, phf1-deficient phenotypes, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the exceptional toxicity of arsenate, As(V), is derived from its close chemical similarity to phosphate, which allows the metalloid to be easily incorporated into plant cells through the high-affinity Pi transport system
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) interacts with C-terminal end of sodium-dependent phosphate transporter 2a
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: the high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the organism requires a functional phosphate import system for infection of hosts via the urinary tract, Pst negatively regulates biofilm formation and pathogenesis. The mutants show an altered proteome compared to the wild-type cells
Products: -
Products: -
?
additional information
?
-
-
Substrates: the high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the organism requires a functional phosphate import system for infection of hosts via the urinary tract, Pst negatively regulates biofilm formation and pathogenesis. The mutants show an altered proteome compared to the wild-type cells
Products: -
Products: -
?
additional information
?
-
-
Substrates: the phosphate transporter PiT-2 is involved in developmental regulation in renal tubules
Products: -
Products: -
?
additional information
?
-
-
Substrates: N-terminal SPX domain of Pho90 interacts physically with regulatory protein Spl2
Products: -
Products: -
?
additional information
?
-
-
Substrates: Spl2 interacts with the N-terminal SPX domain of Pho87 and Pho90
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: Spl2 interacts with the N-terminal SPX domain of Pho87 and Pho90
Products: -
Products: -
?
additional information
?
-
Substrates: PT4 is invlved in nutrient supply by mycorrhiza and mycorrhiza-specific phosphate transport in Solanaceae plants, regulation, overview, comparison to other Pht1 family phosphate transporters in mycorrhizal Pi transport
Products: -
Products: -
?
additional information
?
-
-
Substrates: PT4 is invlved in nutrient supply by mycorrhiza and mycorrhiza-specific phosphate transport in Solanaceae plants, regulation, overview, comparison to other Pht1 family phosphate transporters in mycorrhizal Pi transport
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
Substrates: PT4 is invlved in nutrient supply by mycorrhiza and mycorrhiza-specific phosphate transport in Solanaceae plants, regulation, overview, comparison to other Pht1 family phosphate transporters in mycorrhizal Pi transport
Products: -
Products: -
?
additional information
?
-
-
Substrates: PT4 is invlved in nutrient supply by mycorrhiza and mycorrhiza-specific phosphate transport in Solanaceae plants, regulation, overview, comparison to other Pht1 family phosphate transporters in mycorrhizal Pi transport
Products: -
Products: -
?
additional information
?
-
-
Substrates: co-existence of a high and a low affinity system
Products: -
Products: -
?
additional information
?
-
-
Substrates: PstS is involved in penicillin resistance of Streptococcus pneumoniae
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
additional information
?
-
Substrates: differential regulation of the five gene encoding Pht1 phosphate transporters in Zea mays, involvement of the encoded proteins in diverse processes, including phosphate uptake from soil and transport at the symbiotic interface in mycorrhizas, phosphate (re)translocation in the shoot, and phosphate uptake during pollen tube growth, overview
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Na+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5'-p-fluorosulfonylbenzoyl adenosine
-
pH 7.4, 30 min at 30°C, 1-3 mM
carbonylcyanide 3-chlorophenylhydrazone
-
inhibits phosphate uptake in vivo
guanidine hydrochloride
-
5 min at 100°C, 8000 mM, significant reduction in the activity
phosphite
-
competitive, partial inhibition of phosphate transport activity, overview
phosphonate
-
competitive, partial inhibition of phosphate transport activity, overview
Selenite
competitively inhibits phosphate uptake; competitively inhibits phosphate uptake; competitively inhibits phosphate uptake; competitively inhibits phosphate uptake
arsenic acid
-
As(V) represses the activation of genes specifically involved in Pi uptake, while inducing others transcriptionally regulated by As(V), suggesting that converse signaling pathways are involved in plant responses to As(V) and low phosphate availability, As(V) downregulates genes transcriptionally regulated by Pi starvation, being particularly efficient in the repression of the Pi/As(V) uptake system. The repression occurs conversely to the activation of As(V)-responsive genes, overview
arsenic acid
-
As(V) downregulates genes transcriptionally regulated by Pi starvation, being particularly efficient in the repression of the Pi/As(V) uptake system. The repression occurs conversely to the activation of As(V)-responsive genes, overview
methylphosphonate
-
competitive, partial inhibition of phosphate transport activity, overview
additional information
-
phosphate deficiency results in significant reductions in the total shoot phosphate accumulation in all genotypes. The shoot fresh weight of the wild type grown on arsenate is 42% compared with that of untreated wild-type seedlings
-
additional information
-
no inhibition with sodium azide, sodium orthovanadate and N-ethylmaleimide
-
additional information
-
genes pstS and pstA expression is regulated by the two-component regulatory system PhoBR and is repressed until times of phosphate starvation
-
additional information
-
poor inhibition by ethyl phosphonate, methyl phosphonate, and aminoethyl phosphonate at 0.004-0.04 mM
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
phosphate deficient growth conditions increase the enzyme activity 50fold due to reduced Km for phosphate and higher coupling efficiency between ATP hydrolysis and proton pumping in phosphate-deficient plants
-
additional information
-
phosphate-limitation induces expression of phnD and pstS
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.312
ATP
-
mutant lacking N-terminal SPX domain DELTA1-375, pH and temperature not specified in the publication
0.654
ATP
-
wild-type, pH and temperature not specified in the publication
0.02
phosphate
pH 6.0, 30°C, Vmax: 860 pmol of phosphate incorporated per min/OD650
0.025
phosphate
pH and temperature not specified in the publication, iPT belongs to the high affinity phosphate transporter family
0.025
phosphate
wild-type, pH 6.0, 30°C, Vmax: 440 pmol of phosphate incorporated per min/OD650
0.025
phosphate
using triple mutant DELTApho87-DELTApho90-DELTApho91, pH 6.0, 30°C, Vmax: 2000 pmol of phosphate incorporated per min/OD650
0.0448
phosphate
-
mutant S124A, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 47 nmol/mg of protein/h
0.0462
phosphate
-
mutant D382E, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 96 nmol/mg of protein/h
0.0572
phosphate
-
mutant D382A, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 156 nmol/mg of protein/h
0.077
phosphate
-
wild-type, pH 6.5, 25°C, in the presence of 25 mM NaCl
0.103
phosphate
-
mutant R228K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 274 nmol/mg of protein/h
0.109
phosphate
-
mutant S124T, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 254 nmol/mg of protein/h
0.161
phosphate
-
mutant R120K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 250 nmol/mg of protein/h
0.214
phosphate
-
mutant R201K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 100.7 nmol/mg of protein/h
0.27
phosphate
-
mutant S124A, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 62 nmol/mg of protein/h
0.49
phosphate
-
mutant D382A, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 119 nmol/mg of protein/h
0.73
phosphate
-
mutant D382E, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 132 nmol/mg of protein/h
0.84
phosphate
-
wild-type, pH 6.5, 25°C, in the presence of various concentrations of NaCl
0.88
phosphate
-
mutant S124T, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 222 nmol/mg of protein/h
1.48
phosphate
-
mutant R120K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 125 nmol/mg of protein/h
1.99
phosphate
-
mutant R228K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 221 nmol/mg of protein/h
2.29
phosphate
-
mutant R201K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 126 nmol/mg of protein/h
0.0019
phosphate/out
pH 7.5, temperature not specified in the publication
0.0031
phosphate/out
-
pH and temperature not specified in the publication
0.0091
phosphate/out
-
isozyme HvPHT1;1, pH and temperature not specified in the publication
0.023
phosphate/out
-
isozyme OsPT8, pH and temperature not specified in the publication
0.023
phosphate/out
-
isozyme OsPT8, pH and temperature not specified in the publication
0.0352
phosphate/out
-
pH and temperature not specified in the publication
0.097
phosphate/out
-
isozyme OsPT6, pH and temperature not specified in the publication
0.097
phosphate/out
-
isozyme OsPT6, pH and temperature not specified in the publication
0.13 - 0.28
phosphate/out
-
pH and temperature not specified in the publication
0.192
phosphate/out
-
pH and temperature not specified in the publication
0.385
phosphate/out
-
isozyme HvPHT1;6, pH and temperature not specified in the publication
additional information
additional information
-
phosphate transport kinetics in whole wild-type and mutant cells, overview
-
additional information
additional information
-
OsPT1 follows Michaelis-Menten kinetics
-
additional information
additional information
negative voltage Michaelis-Menten kinetics data, overview
-
additional information
additional information
-
negative voltage Michaelis-Menten kinetics data, overview
-
additional information
additional information
-
phosphate uptake kinetics in planta using radioactive phosphate isotopes
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
expression of the pst genes is 8fold better at pH 5.8 compared to pH 4.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
30
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.6
8.8
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
wild-type strain W115, and mutator line W138 containing an active endogenous dTph1 transposable element system
-
-
brenda
alfalfa symbiont in root nodules, strain 1021, gene pstSCAB
-
-
brenda
the enzyme is encoded by genes pstS and pstA of the pst operon, pstSCAB-phoU
-
-
brenda
the enzyme is encoded by genes pstS and pstA of the pst operon, pstSCAB-phoU
-
-
brenda
wild-type strain R6, ATCC BAA-255, R6 muant strains, and diverse clinical isolates, overview, gene pstS
-
-
brenda
Pht1-1; five genes encoding Pht1 phosphate transporters, mycorrhiza-inducible genes
SwissProt
brenda
Pht1-2; five genes encoding Pht1 phosphate transporters, mycorrhiza-inducible genes
SwissProt
brenda
Pht1-3; five genes encoding Pht1 phosphate transporters, mycorrhiza-inducible genes
SwissProt
brenda
Pht1-4; five genes encoding Pht1 phosphate transporters, mycorrhiza-inducible genes
SwissProt
brenda
Pht1-5; five genes encoding Pht1 phosphate transporters, mycorrhiza-inducible genes
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
localised to the external hyphae of Piriformospora indica colonized with maize plant root, which suggests that external hyphae are the initial site of phosphate uptake from the soil
brenda
endogenous PiT1 mRNA level is decreased in dense MC3T3-E1 cell cultures
brenda
-
proximal, PiT-2: expression analysis in younger and older rats
brenda
-
expression of Pit-1 and Pit-2, Pit-1 is not a component of matrix vesicles or apoptotic bodies, expression analysis, Pit-1 is the predominant sodium-dependent phosphate cotransporter, overview
brenda
additional information
high expression in mature leaves, lower expression in senescent leaves
brenda
transcript is most abundant in young leaves under low phosphate supply
brenda
MPT3:1 highly accumulates in the young leaves and stems under phosphate deficiency
brenda
of mycorrhiza plants and non-mycorrhiza plants
brenda
-
AtPHT1;1, AtPHT1;2, and AtPHT1;4 in the root epidermis and root hairs, alsoin the tip
brenda
enzyme expression occurs principally in the junction area between roots and young nodules
brenda
PHT1;1 is highly expressed in root segments with dense hairs and less root tips
brenda
-
roots and the root-shoot junctions, expression in epidermal, cortical, and stele cells, OsPht1;1 is one of the 13 Pht1 Pi transporters in rice, expressed abundantly and constitutively in various cell types of both roots and shoots independent of phosphate supply condition
brenda
-
isoforms PT1, PT2, PT3, PT4, PT5, PT6, PT8, PT9 and PT10 are preferentially expressed in roots
brenda
-
revealed the presence of LePT1 transcript in the root cap and in the external layers of the root
brenda
of mycorrhiza plants and non-mycorrhiza plants
brenda
-
the shoot fresh weight of the wild type grown on arsenate is 42% compared with that of untreated wild-type seedlings
brenda
-
OsPht1;1is one of the 13 Pht1 Pi transporters in rice, expressed abundantly and constitutively in various cell types of both roots and shoots independent of phosphate supply condition
brenda
-
isoforms PT1, PT2, PT3, PT4, PT5, PT7, PT8, PT6, and PT12 are expressed in shoots
brenda
MPT3:1 highly accumulates in the young leaves and stems under phosphate deficiency
brenda
additional information
PHT4;2 is expressed in multiple sink organs but is nearly restricted to roots during vegetative growth
brenda
additional information
-
expression patterns of the nine PHT1 transporters from Arabidopsis thaliana, overview
brenda
additional information
cell-specific expression pattern of PHT1;1
brenda
additional information
-
the expression pattern of OsPT1 is constitutive independent of phosphate supply, expression analysis of OsPT1 by semi-quantitative RT-PCR
brenda
additional information
PT4 expression analysis, no expression in non-mycorrhizal roots, shoots, and leaves
brenda
additional information
-
PT4 expression analysis, no expression in non-mycorrhizal roots, shoots, and leaves
brenda
additional information
PT4 expression analysis, no expression in non-mycorrhizal roots, shoots, and leaves
brenda
additional information
-
PT4 expression analysis, no expression in non-mycorrhizal roots, shoots, and leaves
brenda
additional information
-
isoform Pht1:7 is highly expressed in the whole potato plants
brenda
additional information
-
expression analysis in wild-type strain R6, R6 muant strains, and diverse clinical isolates, overview
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
both isoforms PT2-GFP and PT8-GFP are retained in the ER when protoplasts are treated with protein phosphatase inhibitor cocktail II
brenda
additional information
the enzyme contains a putative chloroplast transit peptide with a cleavage site between amino acids 59-60
brenda
-
isoforms PHT4:2, PHT4:3 and PHT4:4 localize to the chloroplast envelope
brenda
Oryza sativa Japonica Group Shishoubaimao
-
isoforms PHT4:2, PHT4:3 and PHT4:4 localize to the chloroplast envelope
-
brenda
-
isoforms PHT4:6-1 and PHT4:6-2 colocalize with Golgi markers
brenda
Oryza sativa Japonica Group Shishoubaimao
-
isoforms PHT4:6-1 and PHT4:6-2 colocalize with Golgi markers
-
brenda
-
pstC gene encodes an integral membrane protein, pstC gene encodes a peripheral membrane protein, which carries a putative nucleotide-binding site
brenda
-
MtPT4 and MtPT1, physically continuous with the plasma membrane of the cortical cell, MtPT4 is located only in the domain around the arbuscule branches, targeting of MtPT4 to the periarbuscular membrane, mechanism by precise temporal expression coupled with a transient reorientation of secretion and alterations in the protein cargo entering the secretory system of the colonized root cell, overview
brenda
-
MtPT4 and MtPT1, physically continuous with the plasma membrane of the cortical cell, MtPT4 is located only in the domain around the arbuscule branches, targeting of MtPT4 to the periarbuscular membrane, mechanism by precise temporal expression coupled with a transient reorientation of secretion and alterations in the protein cargo entering the secretory system of the colonized root cell, overview
-
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane
brenda
-
isoform PHT4:1 colocalizes with chloroplast thylakoid
brenda
Oryza sativa Japonica Group Shishoubaimao
-
isoform PHT4:1 colocalizes with chloroplast thylakoid
-
brenda
-
upon phosphate starvation, these low-affinity phosphate transporters, e.g. Pho87 and Pho90, are endocytosed and targeted to the vacuole. For Pho87, this process strictly depends on SPL2, another Pho4-dependent gene that encodes a protein known to interact with the N-terminal SPX domain of the transporter. In contrast, the vacuolar targeting of Pho90 upon phosphate starvation is independent of both Pho4 and Spl2, although it still requires its SPX domain. Both Pho87 and Pho90 are also targeted to the vacuole upon carbon-source starvation or upon treatment with rapamycin, which mimics nitrogen starvation, responses are independent of PHO pathway signalling requiring only the transporter N-terminal SPX domain. Spl2 mediates phosphate-starvation-induced vacuolar targeting of Pho87, but not of Pho90
brenda
-
upon phosphate starvation, these low-affinity phosphate transporters, e.g. Pho87 and Pho90, are endocytosed and targeted to the vacuole. For Pho87, this process strictly depends on SPL2, another Pho4-dependent gene that encodes a protein known to interact with the N-terminal SPX domain of the transporter. In contrast, the vacuolar targeting of Pho90 upon phosphate starvation is independent of both Pho4 and Spl2, although it still requires its SPX domain. Both Pho87 and Pho90 are also targeted to the vacuole upon carbon-source starvation or upon treatment with rapamycin, which mimics nitrogen starvation, responses are independent of PHO pathway signalling requiring only the transporter N-terminal SPX domain. Spl2 mediates phosphate-starvation-induced vacuolar targeting of Pho87, but not of Pho90
-
brenda
additional information
-
provide additional negative charges in the vicinity of the putative ER export site (D/E-X-D/E), thereby altering the recognition of this motif and resulting in the accumulation of PHT1;1 in the endoplasmic reticulum
-
brenda
additional information
PHT1;1 subcellular localization study, overview
-
brenda
additional information
-
PHT1;1 subcellular localization study, overview
-
brenda
additional information
-
during arbuscule formation, secretion of newly synthesized phosphate transporters is directed predominantly toward the developing periarbuscular membrane and not toward the plasma membrane. Subcellular enzyme expression patterns, overview. Correct localization of MtPT4 is regulated by a polarization of the bulk secretory pathway favoring vesicle fusion with the developing periarbuscular membrane rather than with the plasma membrane, and that there is a coincident change in the newly synthesized protein cargo entering into the secretion system
-
brenda
additional information
-
during arbuscule formation, secretion of newly synthesized phosphate transporters is directed predominantly toward the developing periarbuscular membrane and not toward the plasma membrane. Subcellular enzyme expression patterns, overview. Correct localization of MtPT4 is regulated by a polarization of the bulk secretory pathway favoring vesicle fusion with the developing periarbuscular membrane rather than with the plasma membrane, and that there is a coincident change in the newly synthesized protein cargo entering into the secretion system
-
-
brenda
additional information
-
in yeast, several plasma membrane nutrient transporters are inactivated and targeted to the vacuole in response to changes in the availability of nutrients
-
brenda
additional information
-
in yeast, several plasma membrane nutrient transporters are inactivated and targeted to the vacuole in response to changes in the availability of nutrients
-
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
evolution
-
the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview
evolution
-
the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview
evolution
-
the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview
evolution
-
the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview
evolution
-
the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview
evolution
-
the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview
evolution
-
8 PHT1 family genes have been identified from the potato genome
evolution
-
a total of 28 phosphate transporter isoforms are identified, belonging to the PHT1, PHT2, PHT3, CaPHT4, PHT5 and PHO family
evolution
-
phosphate tranbsporter family PHT4 can be classified into six distinct groups. PHT4:1 to PHT4:4 are targeted to chloroplasts, and PHT4:6-1 and PHT4:6-2 are located to Golgi apparatus. PHT4 proteins can mediate inorganic phosphate transport in yeast. They display different expression profiles in response to phosphate starvation, salicylic acid, abscisic acid and salt stress treatments. PHT4 proteins are involved in phosphate distribution between the cytoplasm and chloroplast or Golgi apparatus and also involved in stress responses
evolution
Oryza sativa Japonica Group Shishoubaimao
-
phosphate tranbsporter family PHT4 can be classified into six distinct groups. PHT4:1 to PHT4:4 are targeted to chloroplasts, and PHT4:6-1 and PHT4:6-2 are located to Golgi apparatus. PHT4 proteins can mediate inorganic phosphate transport in yeast. They display different expression profiles in response to phosphate starvation, salicylic acid, abscisic acid and salt stress treatments. PHT4 proteins are involved in phosphate distribution between the cytoplasm and chloroplast or Golgi apparatus and also involved in stress responses
-
malfunction
knockdown transformants of the gene are prepared using electroporation and RNAinterference. Knockdown transformants transport a significantly lower amount of phosphate to the host plant than wild-type. Higher amounts of phosphate are found in plants colonized with wild-type than that of non-colonized and plants colonized with knockdown PiPT
malfunction
selenite uptake is reduced in a DELTA pho84 mutant
malfunction
a triple mutant consisting of deleted low-affinity transporters Pho87, Pho90 and Pho91 is slightly more sensitive to selenite than the wild-type strain. Single mutants do not show any difference to the wild-type stain
malfunction
a triple mutant consisting of deleted low-affinity transporters Pho87, Pho90 and Pho91 is slightly more sensitive to selenite than the wild-type strain. Single mutants do not show any difference to the wild-type strain
malfunction
two independent T-DNA insertion lines from the SALK collection lacking a functional PHT4;2 allele, i.e. pht4;2-1 and pht4;2-2 mutant plants show altered activity and increased biomass due to increased leaf sizes, as well as altered starch accumulation, compared to the wild-type enzyme, phenotypes, overview
malfunction
-
over-expression or knockdown of OsPT1 cause altered phosphate concentration and uptake rate and distribution in phosphate-replete rice, as well as Pi uptake-elicited changes in the root cell membrane potential. Changes in expression affects root growth and root hair development
malfunction
-
the pho84DELTA strain is unable to rapidly reverse phosphate-starvation responses. Deletion of END3, encoding an essential component for endocytosis, which prevents the internalization of Pho87 and, as a consequence, leads to the stabilization of this phosphate transporter at the plasma membrane under phosphate-starving conditions, endocytosis of Pho90 is only prevented in the mutant strain lacking End3 and not in the strains lacking Pho4 or Spl2
malfunction
-
the Arabidopsis accelerated cell death 6-1, acd6-1, mutant shows constitutive defense, cell death, and extreme dwarf phenotype due to a T-DNA disruption in the PHT4;1 gene. The mutant is more susceptible compared to the wild-type to virulent Pseudomonas syringae strains but not to several avirulent strains
malfunction
-
null pht1;9 alleles exhibit exacerbated responses to prolonged phosphate limitation and enhanced tolerance to arsenate exposure, whereas Pht1;9 overexpression induces the opposite phenotypes. Strikingly, Pht1;9 ? Pht1;8 silencing lines display more pronounced defects than the pht1;9 mutants
malfunction
-
Pht1;5-defective mutants show reduced phosphate allocation to shoots and elevated transcript levels for several phosphate starvation-response genes under low-phosphate conditions, under phosphate-replete conditions, pht1;5-1 has higher shoot phosphate content compared to the wild-type but has reduced phosphate content in roots. Overexpression of Pht1;5 affects the distribution and remobilization of phosphate between source and sink. Constitutive overexpression of Pht1;5 has the opposite effect on phosphate distribution: namely, lower phosphate levels in shoots and higher phosphate content in roots compared to the wild-type. Pht1;5 overexpression also results in altered phosphate remobilization, as evidenced by a greater than 2fold increase in the accumulation of phosphate in siliques, premature senescence, and an increase in transcript levels of genes involved in phosphate scavenging. Furthermore, Pht1;5 overexpressors exhibit increased root hair formation and reduced primary root growth that could be rescued by the application of silver nitrate, an ethylene perception inhibitor, or aminoethoxyvinylglycine, an ethylene biosynthesis inhibitor, respectively. Ethylene signaling in modulating the primary root and root hair phenotypes of Pht1;5 overexpression lines, overview
malfunction
-
a phosphorylation-mimicking mutagenesis of PHT1;1 at Ser514 results in its accumulation in the endoplasmic reticulum
malfunction
downregulation of PiT1 severely impairs the proliferation of two transformed human cells lines, HepG2 and HeLa, and the tumorigenicity of HeLa cells in nude mice. An increased PiT1 expression can indeed make NIH3T3 cells more sensitive to transformation. Phenotypes of MC3T3-E1 cells overexpressing hPiT1 or hPiT2, overview
malfunction
PiT1 knock-out mice do not survive past E12.5 and from E10.5, the embryos are growth-retarded and show reduced proliferation of liver cells. Isolated mouse embryonic fibroblasts MC3T3-E1 with knocked out as well as reduced PiT1 expression levels also exhibit impaired proliferation
malfunction
knockdown of isoform PT4 leads to degenerating or dead arbuscule phenotypes
malfunction
knockdown of isoform PT1 leads to degenerating or dead arbuscule phenotypes
malfunction
-
although no alterations in phosphate concentration is in isoform PT9 or PT10 knockdown plants, a significant reduction in phosphate concentration in both shoots and roots is observed in double-knockdown plants grown under both high- and low-phosphate conditions
malfunction
enzyme overexpression gives rise to multiple developmental defects including curly leaves with deep color, dwarfed stature, and reduced fertility. MPT3 overexpressing plants also accumulate higher ATP content, faster respiration rate and more reactive oxygen species than wild type plants
malfunction
-
the Arabidopsis accelerated cell death 6-1, acd6-1, mutant shows constitutive defense, cell death, and extreme dwarf phenotype due to a T-DNA disruption in the PHT4;1 gene. The mutant is more susceptible compared to the wild-type to virulent Pseudomonas syringae strains but not to several avirulent strains
-
malfunction
-
null pht1;9 alleles exhibit exacerbated responses to prolonged phosphate limitation and enhanced tolerance to arsenate exposure, whereas Pht1;9 overexpression induces the opposite phenotypes. Strikingly, Pht1;9 ? Pht1;8 silencing lines display more pronounced defects than the pht1;9 mutants
-
malfunction
-
the pho84DELTA strain is unable to rapidly reverse phosphate-starvation responses. Deletion of END3, encoding an essential component for endocytosis, which prevents the internalization of Pho87 and, as a consequence, leads to the stabilization of this phosphate transporter at the plasma membrane under phosphate-starving conditions, endocytosis of Pho90 is only prevented in the mutant strain lacking End3 and not in the strains lacking Pho4 or Spl2
-
metabolism
phosphate transporter mechanism in the plant cell, overview. When the supply of phosphate is limited, plants grow more roots, increase the rate of phosphate uptake by roots from soil solution, retranslocate phosphate from older leaves, and deplete the vacuolar stores of phosphate. There is also significant retranslocation of phosphate in the phloem from older leaves to the growing shoot and from the shoot to the root
metabolism
-
upon phosphate starvation, these low-affinity phosphate transporters, e.g. Pho87 and Pho90, are endocytosed and targeted to the vacuole. For Pho87, this process strictly depends on SPL2, another Pho4-dependent gene that encodes a protein known to interact with the N-terminal SPX domain of the transporter
metabolism
-
PHT4;1 acts upstream of the SA pathway. PHT4;1 contributes to SID2-dependent and -independent pathways
metabolism
-
the enzyme is involved in arbuscular mycorrhizal, AM, symbiosis,. During arbuscular mycorrhizal symbiosis, the AM fungus colonizes the root cortical cells where it forms branched hyphae called arbuscules that function in nutrient exchange with the plant. Each arbuscule is enveloped in a plant membrane, the periarbuscular membrane, that contains a unique set of proteins including phosphate transporters such as MtPT4 that are essential for symbiotic phosphate transport
metabolism
deprotonation of residue D324 triggers phosphate cytosolic release. Multiple proton transfer events proceed during the transition from the open conformation to the inward conformation. The exit path of the D324 proton requires residue D45
metabolism
-
the alternating conformations of the PstSCAB transporter are sensed by PhoR and PhoU. The sensory mechanism controls the alternate autokinase and phospho-PhoB phosphatase activities of PhoR, which ultimately control the signaling state of the response regulator PhoB
metabolism
residues D35, D38, R134, and D144 are implicated in H+ transfer across the membrane, and Y312 and N421 are involved in initial interaction and translocation of phosphate, all are essential for transport activity. When phsphate enters the binding pocket, the two aromatic moieties of Y145 and F169 and the hydrogen bonds generated from Q172, W304, Y312, D308, and K449 can build a scaffold to stabilize the structure. Subsequent interaction between phosphate and the positive residue of K449 facilitates its release. Residues D38, D93, R134, D144, D212, R216, R233, D367, K373, and E504 may form internal electrostatic interactions for structure ensemble and adaptability
metabolism
-
PHT4;1 acts upstream of the SA pathway. PHT4;1 contributes to SID2-dependent and -independent pathways
-
metabolism
-
the alternating conformations of the PstSCAB transporter are sensed by PhoR and PhoU. The sensory mechanism controls the alternate autokinase and phospho-PhoB phosphatase activities of PhoR, which ultimately control the signaling state of the response regulator PhoB
-
metabolism
-
upon phosphate starvation, these low-affinity phosphate transporters, e.g. Pho87 and Pho90, are endocytosed and targeted to the vacuole. For Pho87, this process strictly depends on SPL2, another Pho4-dependent gene that encodes a protein known to interact with the N-terminal SPX domain of the transporter
-
metabolism
-
phosphate transporter mechanism in the plant cell, overview. When the supply of phosphate is limited, plants grow more roots, increase the rate of phosphate uptake by roots from soil solution, retranslocate phosphate from older leaves, and deplete the vacuolar stores of phosphate. There is also significant retranslocation of phosphate in the phloem from older leaves to the growing shoot and from the shoot to the root
-
metabolism
-
the enzyme is involved in arbuscular mycorrhizal, AM, symbiosis,. During arbuscular mycorrhizal symbiosis, the AM fungus colonizes the root cortical cells where it forms branched hyphae called arbuscules that function in nutrient exchange with the plant. Each arbuscule is enveloped in a plant membrane, the periarbuscular membrane, that contains a unique set of proteins including phosphate transporters such as MtPT4 that are essential for symbiotic phosphate transport
-
metabolism
-
deprotonation of residue D324 triggers phosphate cytosolic release. Multiple proton transfer events proceed during the transition from the open conformation to the inward conformation. The exit path of the D324 proton requires residue D45
-
physiological function
-
high-affinity phosphate uptake system encoded by pst genes is, in addition to its role in phosphate transport, involved in copper and zinc homeostasis
physiological function
-
using the Xenopus laevis expression system it is demonstrated that HvPHT1,6 is a proton-coupled phosphate transporter, though it has time-dependent activation at negative membrane potentials with linear concentration dependence similar to voltage-dependent ion channels, it also transports sulfate anions coupled to protons
physiological function
-
N-terminal SPX domain of Pho90 inhibits low-affinity phosphate transport through a physical interaction with regulatory protein Spl2
physiological function
-
phosphorylation of the sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) plays a key role in the regulation of renal phosphate transport by parathyroid hormone and dopamine. The substitution of serine for aspartic acid (S77D) in the PDZ I domain of NHERF-1 decreases the binding affinity to Npt2a
physiological function
-
NPT1 is a polyspecific anion transporter. Proteoliposomes containing purified SLC17A1 transport various organic anions such as p-aminohippuric acid, acetylsalicylic acid and urate in an inside positive membrane potential (DELTA PSI-dependent manner)
physiological function
when cells are grown at low phosphate concentrations, the high-affinity phosphate transporter Pho84p is the major contributor to selenite uptake. Pho84p is very selective for phosphate as compared to selenite
physiological function
when phosphate is abundant, selenite is internalized through the low-affinity phosphate transporters (Pho87p, Pho90p, and Pho91p). Low-affinity transporters discriminate less efficiently between selenite and phosphate
physiological function
PHT4;2 contributes to phosphate transport in isolated root plastids
physiological function
GmPT1 is a low-affinity phosphate transporter, the enzyme is important in phosphate uptake by roots and translocation within the plant, presumed to occur via a phosphate/proton cotransport mechanism
physiological function
GmPT2 is a low-affinity phosphate transporter, the enzyme is important in phosphate uptake by roots and translocation within the plant, presumed to occur via a phosphate/proton cotransport mechanism
physiological function
-
constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-repleted rice plants
physiological function
-
Pho87 and Pho90 are low-affinity phosphate transporters having differential roles. They represent non-redundant phosphate transporters, which are tuned by the integration of multiple nutrient signalling mechanisms in order to adjust phosphate-transport capacity to the general nutritional status of the environment. Pho90 is the most important phosphate transporter under high phosphate conditions in the absence of a high-affinity phosphate-transport system. Pho84 is the major phosphate transporter in Saccharomyces cerevisiae involved in rapid phosphate signalling
physiological function
-
the phosphate transporter PHT4;1 is critical for basal defense and also implicate a potential role of the circadian clock in regulating innate immunity of Arabidopsis thaliana
physiological function
-
the Pht1;9 and Pht1;8 transporters play similar roles during phosphate deficiency and arsenate exposure, they participate in phosphate acquisition and arsenate uptake. Plasma membrane-localized transporter Pht1;9 mediates high-affinity phosphate ?H+ symport activity and is highly induced in phosphate-starved Arabidopsis roots
physiological function
HvPHT1;1 is a high-affinity phosphate transporter involved in uptake of phosphate from soil solution under low phosphate conditions
physiological function
-
Arabidopsis thaliana Pht1;5 is implicated in mobilizing stored phosphate out of older leaves, it mobilizes phosphate between source and sink organs and influences the interaction between phosphate homeostasis and ethylene signaling. Pht1;5 is involved in the mobilization of phosphate from shoots to roots during high-phosphate conditions, remobilization of phosphate from senescing to metabolically active parts of the plant
physiological function
a certain level of PiT1 is important for proliferation, role of PiT1 in regulation of cell proliferation, overview
physiological function
a certain level of PiT1 is important for proliferation, role of PiT1 in regulation of cell proliferation, overview
physiological function
-
the enzyme contributes to the enhanced arsenate uptake capacity and affinity exhibited by Pteris vittata
physiological function
isoform PT4 is required for symbiotic phosphate uptake
physiological function
-
isoforms PT9 and PT10 redundantly function in phosphate uptake
physiological function
the high-affinity phosphate transporter PT5 regulates phosphate transport to nodules and nodulation in soybean. The enzyme controls phosphate entry from roots to nodules, is critical for maintaining phosphate homeostasis in nodules, and subsequently regulates soybean nodulation and growth performance
physiological function
-
the enzyme is involved in the translocation of phosphate from the root to the shoot but not from the soil solution into the root
physiological function
-
under both phosphate sufficient and deficient conditions, transgenic plants constitutively expressing the enzyme grew taller than the non-transformed wild type, produce a greater volume of roots, accumulate more biomass and take up more phosphate
physiological function
-
phosphate transporter PHT4;1 is a salicylic acid regulator acting independently of several known salicylic acid genes
physiological function
-
overexpression of the rice phosphate transporter gene PT2 enhances tolerance to low phosphorus stress in soybean and improves phosphate acquisition and seed yield
physiological function
the enzyme plays an important role in chloroplast phosphate compartmentation and ATP synthesis, which affect plant growth, and also maintains the ionic environment of thylakoids
physiological function
-
cells lacking PstSCAB survive poorly in stationary phase, in nutrient-limiting media, and under osmotic conditions reflective of those in the chicken. Polyphosphate levels in the mutant cells are elevated at stationary phase. The mutant strain is highly attenuated for colonization of newly hatched chicks, with levels of bacteria at several orders of magnitude below wild-type levels. Mutant and wild type grow similarly in complex media, but the PstS disrupotion mutant exhibits a significant growth defect in minimal medium supplemented with L-lactate
physiological function
-
overexpression of PHT1:4 in soybean promotes the increase of plant biomass and yield of transgenic plants upon low phosphate stress, and increases the accumulation and transportation of phosphate from roots to leaves in the transgenic soybean lines
physiological function
-
overexpression of isoform PHT1:7 (with a relative expression 2-7-fold that in the control) and silencing (with a relative expression of 0.3-1% that in the control). The growth vigor is ranked in the order overexpression line > wild type > silenced line. In the absence of phosphorus, the root length of the overexpression line is approximately 2.6 times that of the wild-type, while the root length of the silenced line is approximately 0.6 times that of the wild type. The tolerance to drought stress is ranked as wild type > overexpression line > silenced line
physiological function
A0A290QKL9
Staphylococcus aureus possesses three distinct Pi transporters: PstSCAB, PitA, and NptA. The loss of a single transporter does not affect S. aureus. Disruption of any two systems significantly reduces phosphate accumulation and growth in divergent environments. In a systemic mouse model of disease, loss of any one transporter does not decrease staphylococcal virulence. Loss of NptA in conjunction with either PstSCAB or PitA significantly reduces the ability of S. aureus to cause infection
physiological function
PHT1:7 enhances tolerance to limited phosphate nutrients in apple calli. Expression of PHT1:7 complements a yeast mutant lacking PHO84 expression. Overexpression of PHT1:7 enhances phosphate tolerance in tomato, transgenic plants show increased drought tolerance
physiological function
Pht1:3 overexpression in Arabidopsis thaliana enhances absorption of phosphate and increases biomass of the transgenic plants
physiological function
expression of MPT3:1 compensates the phosphate uptake in a yeast MB192 mutant is defective in phosphate transport under phosphate deficiency. Overexpression of MPT3:1 in Oryza sativa promotes phosphate uptake by the roots, and increases the translocation of phosphate from the roots to the shoots. The transgenic rice accumulates more chlorophyll and soluble sugar in the shoots than the wild-type under phosphate deficiency. Overexpression leads to an increase in tiller number and effective panicle of per plant, and increasing grain yield under phosphate deficiency
physiological function
overexpression of PT8 suppresses rice disease resistance against the pathogens Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae. In PT8-overexpressing plants, pathogen-associated molecular patterns (PAMPs)-triggered immunity (PTI) response genes, such as Rac1 and SGT1, are suppressed during treatment with PAMPs chitin or flg22. PT8 is an interactor of a rice mitogen-activated protein kinase BWMK1. Under low phosphate conditions, the overexpressing plants display better agronomic traits than the control plants
physiological function
both wild-type and strains lacking PitB are able to internalize tellurite into the cytoplasm and reduce the oxyanion to black nano-sized and rod-shaped tellurium particles. The strain lacking PitB shows an increased resistance to the tellurite toxic effects. At 0.1 mM tellurite, the biomass formation of the wild-type strain decreases by half, and the mutant strain shows greater ability to reduce tellurite with respect to the wild-type, which is related to the greater biomass production of the mutant
physiological function
-
vacuolar phosphate transporter VPT1 is associated with As(V) tolerance. VPT1 mutants display enhanced tolerance to As(V) toxicity, whereas plants overexpressing VPT1 are more sensitive to As(V) as compared with the wild-type plants. Many of the plasma membrane phosphate transporter family PHT1 genes are down-regulated in the VPT1 mutant as compared with the wild type under phosphate-sufficient conditions, but not when phosphate levels are low in the medium. As(V) resistance in VPT1 mutants is prominent only under phosphate-sufficient but not under phosphate-deficient conditions
physiological function
-
transcription activator MYB5P directly binds to MYB binding site motifs on the PT5 promoter and induces transcription of PT5 in rice. Growth of the shoot and primary root in an PT5 mutant is more inhibited than in wild-type plants in high-phosphate and phosphate-deficient media. The fresh weights of PT5-deficient and MYB5P-RNAi plants are comparable. There is no difference in the phosphate content of roots and shoots of PT5 mutant and MYB5P-RNAi plants grown in high-phosphate and phosphate-deficient media. Overexpression of MYB5P leads to increased phosphate accumulation in shoots and roots
physiological function
-
Pt9-overexpressing roots are difficult to colonize by arbuscular mycorrhiza fungi. Pt9 complements a yeast mutant defective in phosphate transporter activity and improves the phosphate concentration in rice. Pt9 is involved in phosphate accumulation under both phosphate-deficient and phosphate-sufficient conditions
physiological function
-
vacuolar phosphate transporters, VPT1 and VPT3 contribute to cytosol-to-vacuole phosphate partitioning. VPT1 plays a predominant role, and VPT3 is particularly important when VPT1 is absent. The VPT1 VPT3 double mutant is more defective in phosphate homeostasis than the VPT1 single mutant. VPT2 does not appear to contribute to phosphate homeostasis under these conditions. The VPT1 VPT3 double mutant is impaired in reproductive development with shortened siliques and impaired seed set under sufficient phosphate and displays phosphate overaccumulation in the floral organs. Excess phosphate in the pistil is inhibitory to pollen tube growth, and thus seed yield, in the mutant plants
physiological function
-
PHT2;1 functions as a low-affinity phosphate transporter. A PHT2;1 mutant has reduced phosphate accumulation, plant growth and photosynthetic rates. 52.6% of the decreased metabolites in mutant plants are flavonoids, i.e. a 40% lower content of total flavonoids compared with the wild type. Mutant plants are more sensitive to UV-B irradiation and have a reduced content of phenylalanine, largely associated with the repressed expression of ADT1/MTR1. Mutant plants show decreased grain yields at relatively high levels of UV-B irradiance
physiological function
-
phosphate transporters PT2 and PT8 interact with protein phosphatase type 2C PP95. PP95 dephosphorylates PT8 at Ser517. Rice plants overexpressing PP95 show reduced PT8 phosphorylation and promote PT2 and PT8 trafficking from the ER to the plasma membrane, resulting in phosphate accumulation. Under phosphate-sufficient conditions, phosphate levels are lower in young leaves and higher in old leaves in PP95 mutants than in those of the wild-type, while the overall shoot phosphate levels are the same in the mutant and the wild-type. In the wild-type, PP95 accumulates under phosphate starvation but is rapidly degraded under phosphate-sufficient conditions
physiological function
-
the phosphate transporter PHT4;1 is critical for basal defense and also implicate a potential role of the circadian clock in regulating innate immunity of Arabidopsis thaliana
-
physiological function
-
the Pht1;9 and Pht1;8 transporters play similar roles during phosphate deficiency and arsenate exposure, they participate in phosphate acquisition and arsenate uptake. Plasma membrane-localized transporter Pht1;9 mediates high-affinity phosphate ?H+ symport activity and is highly induced in phosphate-starved Arabidopsis roots
-
physiological function
-
cells lacking PstSCAB survive poorly in stationary phase, in nutrient-limiting media, and under osmotic conditions reflective of those in the chicken. Polyphosphate levels in the mutant cells are elevated at stationary phase. The mutant strain is highly attenuated for colonization of newly hatched chicks, with levels of bacteria at several orders of magnitude below wild-type levels. Mutant and wild type grow similarly in complex media, but the PstS disrupotion mutant exhibits a significant growth defect in minimal medium supplemented with L-lactate
-
physiological function
-
Pho87 and Pho90 are low-affinity phosphate transporters having differential roles. They represent non-redundant phosphate transporters, which are tuned by the integration of multiple nutrient signalling mechanisms in order to adjust phosphate-transport capacity to the general nutritional status of the environment. Pho90 is the most important phosphate transporter under high phosphate conditions in the absence of a high-affinity phosphate-transport system. Pho84 is the major phosphate transporter in Saccharomyces cerevisiae involved in rapid phosphate signalling
-
physiological function
-
Staphylococcus aureus possesses three distinct Pi transporters: PstSCAB, PitA, and NptA. The loss of a single transporter does not affect S. aureus. Disruption of any two systems significantly reduces phosphate accumulation and growth in divergent environments. In a systemic mouse model of disease, loss of any one transporter does not decrease staphylococcal virulence. Loss of NptA in conjunction with either PstSCAB or PitA significantly reduces the ability of S. aureus to cause infection
-
physiological function
-
GmPT1 is a low-affinity phosphate transporter, the enzyme is important in phosphate uptake by roots and translocation within the plant, presumed to occur via a phosphate/proton cotransport mechanism
-
physiological function
-
GmPT2 is a low-affinity phosphate transporter, the enzyme is important in phosphate uptake by roots and translocation within the plant, presumed to occur via a phosphate/proton cotransport mechanism
-
physiological function
Oryza sativa Dongjin
-
transcription activator MYB5P directly binds to MYB binding site motifs on the PT5 promoter and induces transcription of PT5 in rice. Growth of the shoot and primary root in an PT5 mutant is more inhibited than in wild-type plants in high-phosphate and phosphate-deficient media. The fresh weights of PT5-deficient and MYB5P-RNAi plants are comparable. There is no difference in the phosphate content of roots and shoots of PT5 mutant and MYB5P-RNAi plants grown in high-phosphate and phosphate-deficient media. Overexpression of MYB5P leads to increased phosphate accumulation in shoots and roots
-
additional information
-
OsPT1 expression is not significantly regulated by phosphate-supply level in the transgenic rice plants. Expression of OsPT1 is upregulated in rice phosphate accumulator mutant (ospho2) and not altered in phosphate starvation responsive mutant (osphr2)
additional information
-
recombinant human NELL-1 and BMP-2 significantly increase phosphate transport and recombinant human NELL-1 regulates Pit transporters, detailed overview
additional information
-
the SPX domain is essential for vacuolar targeting of Pho87 and Pho90 in response to glucose starvation and rapamycin treatment
additional information
-
PHT4;1 expression is Rregulated by light and the circadian clock, overview. PHT4;1-1 exhibits a similar diurnal expression pattern, with peaks in the daytime and troughs in the nighttime. Treatment with a salicylic acid agonist induce a similar level of resistance against pathogens in wild-type and mutant PHT4;1
additional information
-
transcriptional regulaiton of the PHT1 transporters, variation of phosphate concentration in the medium promotes a rapid modulation of PHT1 transcripts, overview
additional information
isoform PT1 is not required for symbiotic phosphate uptake
additional information
isoform PT1 is not required for symbiotic phosphate uptake
additional information
-
isoform PT1 is not required for symbiotic phosphate uptake
additional information
-
PHT4;1 expression is Rregulated by light and the circadian clock, overview. PHT4;1-1 exhibits a similar diurnal expression pattern, with peaks in the daytime and troughs in the nighttime. Treatment with a salicylic acid agonist induce a similar level of resistance against pathogens in wild-type and mutant PHT4;1
-
additional information
-
the SPX domain is essential for vacuolar targeting of Pho87 and Pho90 in response to glucose starvation and rapamycin treatment
-
Show AA Sequence and Transmembrane Helices (418 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35000
x * 35000, SDS-PAGE, x * 50500, about, processed enzyme, sequence calculation
45000
x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE
50500
x * 35000, SDS-PAGE, x * 50500, about, processed enzyme, sequence calculation
50700
x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE
56000
56100
x * 56100, sequence calculation, Pht1-5
56500
x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE
58300
x * 58300, sequence calculation, Pht1-3
58400
x * 58400, sequence calculation, Pht1-4
58800
60600
x * 60600, sequence calculation, Pht1-6
58800
x * 58800, sequence calculation, Pht1-1
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
additional information
?
x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE
?
x * 35000, SDS-PAGE, x * 50500, about, processed enzyme, sequence calculation
?
-
calculation from nucleotide sequence, PstB is the ATP binding component, PstA/PstC are two hydrophobic subunits of phosphate permease belonging to the family of ABC transporters, PstS-1 is the phosphate binding subunit
?
x * 58800, sequence calculation, Pht1-1
?
x * 58800, sequence calculation, Pht1-2
?
x * 58300, sequence calculation, Pht1-3
?
x * 58400, sequence calculation, Pht1-4
?
x * 56100, sequence calculation, Pht1-5
?
x * 60600, sequence calculation, Pht1-6
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
additional information
-
PstS is the substrate-binding component, PstBA and PstBB are the membrane-associated ATP binding components
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
additional information
-
tertiary structure of PstB, conformational and structural changes upon nucleotide binding analysis, structure modeling, overview
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
additional information
-
three-dimensional structure homology modelling using the periplasmic domain GP-56, overview
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
additional information
-
PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
the periplasmic domain GP-56 is N-and O-glycosylated, identification of 6 predicted N-glycosylation sites and two predicted O-glycosylation sites, Thr273 and Thr306, overview
no modification
phosphoprotein
phosphoprotein
-
phosphory lation events occur at the C-end of PHT1 proteins when phosphate is abundant in the environment. Phosphorylation of other Arabidopsis thaliana members of the family, PHT1;4, PHT1;5; PHT1;7, and PHT1;9, at the C-end of the protein
phosphoprotein
-
protein phosphatase PP95 dephosphorylates PT8 at Ser517
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology modelling of phosphate transporter isoforms PHT1, PHT2, PHT3, PHT4, PHT5 and of the PHO family
-
in complex with phosphate, sitting drop vapor diffusion method, using 0.1 M sodium acetate, 0.2 M zinc acetate pH 4.5, and 10% (w/v) PEG 3000
homology modeling and molecular docking of As/phosphate. Phosphate and arsenate ions bind at the N-terminal of the SPX domain. Residues Lys3, Ph4, Ser5 and Lys27 show interaction with the phosphate. Val21, Trp24 and Glu25 of the SPX domain interact with arsenate
A0A0K9RUE0
structure in complex with sodium and phosphate at 2.3 A resolution. One phosphate and two sodium ions are located at the core of PiT and the third sodium ion (Nafore) is located near the inner membrane boundary. An elevator-like mechanism may account for sodium and phosphate transport by TiT, with the PiT-Na/phosphate complex adopting an inward occluded conformation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D144A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
D191A
residue predicted to face the extracellular side, 40% of wild-type activity in a yeast Pam2 complementation assay
D212A
residue predicted to face the extracellular side, 40% of wild-type activity in a yeast Pam2 complementation assay
D228E
-
drastic increase in expression compared to wild-type, mutant shows no transport activity
D308A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
D35A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
D367A
residue predicted to face the cytoplasm side, 40-50% of wild-type activity in a yeast Pam2 complementation assay
D382A
-
Km: (phosphate) 0.057 mM (in the presence of 25 mM NaCl), 0.49 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 156 nmol/mg of protein/h, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity
D382E
-
Km: (phosphate) 0.046 mM (in the presence of 25 mM NaCl), 0.73 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 96 nmol/mg of protein/h, drastic increase in expression compared to wild-type, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity
D38A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
D93A
residue predicted to face the cytoplasm side, 40-50% of wild-type activity in a yeast Pam2 complementation assay
E122A
residue predicted to face the extracellular side, 80% of wild-type activity in a yeast Pam2 complementation assay
E504A
residue predicted to face the cytoplasm side, 40-50% of wild-type activity in a yeast Pam2 complementation assay
F169A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
K124A
residue predicted to face the extracellular side, 60% of wild-type activity in a yeast Pam2 complementation assay
K16A
residue predicted to face the cytoplasm side, 40-50% of wild-type activity in a yeast Pam2 complementation assay
K373A
residue predicted to face the cytoplasm side, 60-70% of wild-type activity in a yeast Pam2 complementation assay
K449A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
K482A
residue predicted to face the extracellular side, 80% of wild-type activity in a yeast Pam2 complementation assay
K99A
residue predicted to face the cytoplasm side, 60-70% of wild-type activity in a yeast Pam2 complementation assay
N421A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
Q172A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
R120K
-
Km: (phosphate) 0.161 mM (in the presence of 25 mM NaCl), 1.48 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 250 nmol/mg of protein/h, results indicate that Arg-120 may be important for binding and translocation of the substrate
R134A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
R201K
-
Km: (phosphate) 0.214 mM (in the presence of 25 mM NaCl), 2.29 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 100 nmol/mg of protein/h, results indicate that Arg-201 may be important for binding and translocation of the substrate
R216A
residue predicted to face the extracellular side, 40% of wild-type activity in a yeast Pam2 complementation assay
R228K
-
drastic increase in expression compared to wild-type, Km: (phosphate) 0.103 mM (in the presence of 25 mM NaCl), 1.99 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 274 nmol/mg of protein/h, drastic increase in expression compared to wild-type, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity
R233A
residue predicted to face the cytoplasm side, 40-50% of wild-type activity in a yeast Pam2 complementation assay
R345A
residue predicted to face the extracellular side, 60% of wild-type activity in a yeast Pam2 complementation assay
R402A
residue predicted to face the extracellular side, 40% of wild-type activity in a yeast Pam2 complementation assay
S124A
-
Km: (phosphate) 0.0448 mM (in the presence of 25 mM NaCl), 0.27 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 47 nmol/mg of protein/h, results indicate that Ser-124 may function as a transient binding site for Na+ ions in close proximity to the periplasmic side
S124T
-
Km: (phosphate) 0.109 mM (in the presence of 25 mM NaCl), 0.88 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 254 nmol/mg of protein/h, results indicate that Ser-124 may function as a transient binding site for Na+ ions in close proximity to the periplasmic side
W304A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
Y145A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
Y312A
mutation of residue within the transmembrane helix, partial complementation of a yeast Pam2 mutnat strain
E179Q
-
mutation predicted to lock the PstB protein in either of two conformations. Mutation is predicted to reside in an outward-facing, closed conformation and signals phosphate starvation. Mutant does not show phosphate transport
E240Q
-
mutation in pstC, loss of phosphate transport through the pst system, alkaline phosphatase activity remains repressed
G48I
-
mutation in pstB, loss of phosphate transport through the Pst system and derepression of alkaline phosphatase activity
K49Q
-
mutation in pstB, loss of phosphate transport through the Pst system and derepression of alkaline phosphatase activity
P123L/P166L
-
mutation in pstA, complete loss of phosphate transport activity
Q160K
-
mutation predicted to lock the PstB protein in either of two conformations. Mutation is predicted to reside in an inward-facing, open conformation and signals phosphate sufficiency. Mutant does not show phosphate transport
R238Q
-
mutation in pstC, loss of phosphate transport through the pst system, alkaline phosphatase activity remains repressed
R238Q/E240Q
-
mutation in pstC, loss of phosphate transport through the pst system, alkaline phosphatase activity remains repressed
E179Q
-
mutation predicted to lock the PstB protein in either of two conformations. Mutation is predicted to reside in an outward-facing, closed conformation and signals phosphate starvation. Mutant does not show phosphate transport
-
Q160K
-
mutation predicted to lock the PstB protein in either of two conformations. Mutation is predicted to reside in an inward-facing, open conformation and signals phosphate sufficiency. Mutant does not show phosphate transport
-
S115F
-
mutant MtPT4S115F is retained in the endomembrane system and colocalizes with endoplasmic reticulum and trans-Golgi network markers. Mutation of this residue disrupts a conserved process in phosphate transporter trafficking
S117F
-
mutant MtPT1S117F is retained in the endomembrane system and colocalizes with endoplasmic reticulum and trans-Golgi network markers. Mutation of this residue disrupts a conserved process in phosphate transporter trafficking
S115F
-
mutant MtPT4S115F is retained in the endomembrane system and colocalizes with endoplasmic reticulum and trans-Golgi network markers. Mutation of this residue disrupts a conserved process in phosphate transporter trafficking
-
S117F
-
mutant MtPT1S117F is retained in the endomembrane system and colocalizes with endoplasmic reticulum and trans-Golgi network markers. Mutation of this residue disrupts a conserved process in phosphate transporter trafficking
-
L175A
mitochondrial transport of phosphate but not of Cu is compromised in the mutant. L175A mutant protein is present in mitochondria and increases steady-state COX1 levels
R138A
-
mutant possesses Na+/phosphate cotransport activity comparable to that of the wild type protein, but the DELTA PSI-dependent anion transport is inactive
D188K
DELTA1-375
-
mutant lacking the entire N-terminal SPX domain shows no differences in protein level or plasma membrane localisation compared to wild-type, phosphate uptake-rate is higher compared to wild-type, Km remains in the same range as wild-type, truncated mutant has increased catalytic activity
D45E
mutant has a Km similar to wild-type, but the Vmax is lowered 50fold below wild type
D45N
D45E
-
mutant has a Km similar to wild-type, but the Vmax is lowered 50fold below wild type
-
D45N
D22A
mutant maintains phosphate-binding affinities, but shows phosphate uptake defects
D258A
mutant maintains phosphate-binding affinities, but shows phosphate uptake defects
D22A
-
mutant maintains phosphate-binding affinities, but shows phosphate uptake defects
-
D258A
-
mutant maintains phosphate-binding affinities, but shows phosphate uptake defects
-
additional information
D45N
mutant has an about 10fold lower Km and 5fold lower Vmax value compared with wild-type PiPT
D45N
-
mutant has an about 10fold lower Km and 5fold lower Vmax value compared with wild-type PiPT
-
additional information
-
overexpression of the s(V)-tolerant missense mutant pht1;1-3 results in decreased phosphate content and enhanced arsenic accumulation, the mutant accumulates double the arsenic found in wild-type plants, the As(V) tolerance phenotypes are enhanced in the pht1;1-3 expressor lines, pht1;1-3 exhibits a constitutive phosphate starvation response, overview
additional information
-
heterologous functional expression of the Pht1;9 transporter in Saccharomyces cerevisiae strain BY4741 DELTApho84 mutant, which shows growth limitation under phosphate starvation, and complementation, expression?subcellular localization studies, reverse genetics approaches in planta, and generation of double Pht1;9?Pht1;8 silencing lines
additional information
-
construction of Pht1;5 deletion and overexpression mutants, phenotypes, detailed overview. Pht1;5 overexpression alters Root hair development and primary root growth in association with ethylene signaling
additional information
-
heterologous functional expression of the Pht1;9 transporter in Saccharomyces cerevisiae strain BY4741 DELTApho84 mutant, which shows growth limitation under phosphate starvation, and complementation, expression?subcellular localization studies, reverse genetics approaches in planta, and generation of double Pht1;9?Pht1;8 silencing lines
-
additional information
-
smooth muscle cells expressing small interfering RNA show decreased Pit-1 mRNA and protein levels and reduced sodium-dependent phosphate transport activity compared with the control transduced cells, phosphate-induced smooth muscle cell calcification is significantly inhibited in smooth muscle cell-siRNA, and the siRNA-cells are not less susceptible to cell death than cells containing normal levels of Pit-1, overexpression of murine wild-type Pit-1 restores phosphate uptake and phosphate-induced calcification in human Pit-1 deficient cells, overview
additional information
downregulation of PiT1 in two transformed human cells lines, HepG2 and HeLa, phenotypes, overview. Overexpression of hPiT1 or hPiT2 in MC3T3-E1 cells, phgenotypes, overview. Transduction study analyzing the presence of functional hPiT1, hPiT2, and mPiT2 on the cell surface of MC3T3-E1-LXSN, -LPiT1SN, -LPiT2SN, NIH3T3-LXSN, and -LPiT1SN cells
additional information
-
downregulation of PiT1 in two transformed human cells lines, HepG2 and HeLa, phenotypes, overview. Overexpression of hPiT1 or hPiT2 in MC3T3-E1 cells, phgenotypes, overview. Transduction study analyzing the presence of functional hPiT1, hPiT2, and mPiT2 on the cell surface of MC3T3-E1-LXSN, -LPiT1SN, -LPiT2SN, NIH3T3-LXSN, and -LPiT1SN cells
additional information
HvPHT1;1 activity analysis in Xenopus laevis oocytes via cRNA-injection, low external sodium concentration is critical for phosphate transporter characterization in Xenopus laevis oocytes
additional information
-
HvPHT1;1 activity analysis in Xenopus laevis oocytes via cRNA-injection, low external sodium concentration is critical for phosphate transporter characterization in Xenopus laevis oocytes
additional information
generation of PiT1 knock-out mice and isolated mouse embryonic fibroblasts with PiT1 knockout
additional information
-
generation of PiT1 knock-out mice and isolated mouse embryonic fibroblasts with PiT1 knockout
additional information
-
construction of deletion mutants, mutation of either pstS and phnD leads to impaired cell growth in minimal medium, the cells are only viable in high phosphate concentrated medium
additional information
-
construction of deletion mutants, mutation of either pstS and phnD leads to impaired cell growth in minimal medium, the cells are only viable in high phosphate concentrated medium
-
additional information
-
transgenic plants of OsPT1 overexpression lines and RNA-interference knockdown lines contain significantly higher and lower phosphate concentration, respectively, respectively, compared to that of wild-type control. OsPT1 is able to complement the nH+/phosphate co-transporter activities in a yeast mutant MB192 strain defective in phosphate-uptake. No significant difference of phosphate concentration in the roots of OsPT1-Ox1, OsPT1-Ri1 and wild-type irrespective of phosphate supply levels
additional information
-
effective symbiotic phosphate transport in mycorrhizal roots of Petunia is a hidden molecular phenotype, overview. Construction of transposon insertion mutants is established by crossing the transgenic reporter line with the mutator W138 line, from which the phosphate transporter downregulated mutant is identi?ed, which exhibits strongly reduced expression of mycorrhiza-inducible phosphate transporters in mycorrhizal roots
additional information
-
the normal suppression of enzyme expression by the two-component regulatory system PhoBR is relieved in pstS-Tn5 and pstA-Tn5 mutants, which constitutively produce AP regardless of growth conditions, pst mutants show no significant growth defects during the independent culture or coculture studies in rich medium, phosphate-limiting minimal salts medium, or human urine. Mutants complemented with the complete pst operon repress AP synthesis in vitro and colonize the mouse bladder in numbers comparable to the wild-type strain HI4320, overview
additional information
-
phosphate-specific transport mutants, DELTApst, show a defect in biofilm formation when grown in human urine, overview
additional information
-
the normal suppression of enzyme expression by the two-component regulatory system PhoBR is relieved in pstS-Tn5 and pstA-Tn5 mutants, which constitutively produce AP regardless of growth conditions, pst mutants show no significant growth defects during the independent culture or coculture studies in rich medium, phosphate-limiting minimal salts medium, or human urine. Mutants complemented with the complete pst operon repress AP synthesis in vitro and colonize the mouse bladder in numbers comparable to the wild-type strain HI4320, overview
-
additional information
-
phosphate-specific transport mutants, DELTApst, show a defect in biofilm formation when grown in human urine, overview
-
additional information
-
strain PAM1 is transformed with PHO84pht1;1-3, a mutagenized version of the PHO84 cDNA, encoding a Gly-to-Glu mutation identical to the one present in the Arabidopsis thaliana pht1;1-3 allele. In the presence of 550 mM Pi, cells expressing PHO84pht1;1-3 exhibits more phosphatase activity than cells transformed with either PHO84 cDNA or the empty vector PAM1, the rate of Pi and As(V) transport in cells expressing PHO84pht1;1-3 is significantly lower than in the original PAM1, overview
additional information
-
mutation of pstC affects the PstSCAB phosphate transport system resulting in the PhoB-phenotype, overview
additional information
PT4-deficient transgenic mutant tomato plants show a reduced number of roots and shorter lateral roots, overview
additional information
-
PT4-deficient transgenic mutant tomato plants show a reduced number of roots and shorter lateral roots, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
-
pH 7.5, for 5 min, substantial loss in the enzyme activity at temperatures above 37°C
80
80
-
pH 7.5, between 30 and 60 min of heat, maximum loss of enzyme activity, 60% of initial value
80
-
pH 7.5,incubating for 15 min, hardly shows any aggregation and for 1 h, loss 11% of its initial enzyme activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap nickel affinity column chromatography and Superdex 75 gel filtration
native enzyme partially by preparation of chloroplasts and thylakoid membranes
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of genes pstS, pstC, pstA, and pstB from the pst operon, genetic architecture, DNA and amino acid sequence determination and analysis, expression analysis in cells
-
DNA and amino acid sequence determination and analysis, expression analysis, functional overexpression of the enzyme as His6-Xpress-ANTR1-FLAG fusion protein in Escherichia coli, the recombinantly expressed Arabidopsis ANTR1 facilitates Na+-dependent phosphate transport into Escherichia coli
DNA and amino acid sequence determination of the pst operon, genes pstS and pstA expression is regulated by the two-component regulatory system PhoBR and is repressed until times of phosphate starvation
-
expressed in Escherichia coli
expression in transgenic Arabidopsis thaliana plants by Agrobacterium tumefaciens strain EHA105 transfection method, expression of recombinant GFP- and YFP-tagged Pht1;9 in Arabidopsis thaliana protoplasts via polyethylene glycol transformation
-
expression of a mycorrhiza-inducible bifunctional reporter transgene and insertional mutagenesis in Petunia hybrida strain W115, bidirectionalization of a mycorrhizal phosphate transporter promoter StPT3 controlling the expression of two reporter genes encoding firefly luciferase and GUS allows visualization of mycorrhiza-specific phosphate transporter expression
-
expression of HvPHT1;1 in Allium cepa epidermal cell plasma membranes from gateway-enabled pMDC83 vector with HvPHT1;1 being placed in-frame, upstream of the mGFP6 gene
gene PHO84, DNA and amino acid sequence determination and analysis, expression analysis
-
gene pht1-1, computational DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression pattern and regulation, overview
gene pht1-2, computational DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression pattern and regulation, overview
gene pht1-3, computational DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression pattern and regulation, overview
gene pht1-4, computational DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression pattern and regulation, overview
gene pht1-5, computational DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression pattern and regulation, overview
gene pht1-6, computational DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression pattern and regulation, overview
gene PHT1;1 mapped to locus to chromosome V, expression analysis, overview
-
gene pht4;2, DNA and amino acid sequence determination and analysis, genetic organization, PHT4;2 is located adjacent to DEETIOLATED2 (DET2) on chromosome 2 (loci At2g38060 and At2g38050, respectively), with 550 bp between the PHT4;2 stop codon and the DET2 translation start site, quantitative RT-PCR expression analysis, recombinant expression of the processed enzyme
gene pstSCAB, the expression is regulated by PhoB, which binds to the pstSCAB promoter at two binding sites
-
gene PT1, isolation of root-specific promoter sequence of gene PT1 by utilizing the gene-space sequence information, root-specific expression of GFP-tagged GUS-fusion enzymes in Arabidopsis thaliana and in transgenic roots of Medicago truncatula using the Agrobacterium tumefaciens transfection system, expression patterns, overview
gene PT2, isolation of root-specific promoter sequence of gene PT2 by screening of a genomic library, root-specific expression of GFP-tagged GUS-fusion enzymes in Arabidopsis thaliana and in transgenic roots of Medicago truncatula using the Agrobacterium tumefaciens transfection system, expression patterns, overview
GmPT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in yeast MB192 mutant cells, expression as GFP-tagged protein in onion epidermal cells from chimeric gene CaMV35S-GmPT1 introduced into the cells by a particle bombardment system
GmPT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in yeast MB192 mutant cells, expression as GFP-tagged protein in onion epidermal cells from chimeric gene CaMV35S-GmPT1 introduced into the cells by a particle bombardment system
MtPT4-GFP and MtPT1 expression from the MtPT4 and MtPT1 promoter, respectively, in Medicago truncatula plants in the periarbuscular membrane, and from the 35S promoter in roots and in the vacuole of vascular tissue cells. The plasma membrane GFP signal appears substantially weaker in cells harboring arbuscules than in other cortical cells. MtPT1-GFP fusions were expressed from the MtBcp1 promoter, which is active in cortical cells both before and during arbuscule formation. Phenotype of roots expressing pMtBcp1:MtPT4-GFP, overview
-
OsPT1 semi-quantitative RT-PCR and real time quantitative RT-PCR expression analyses
-
pst and phn operons, the Phn system is encoded by a three-gene operon, DNA and amino acid sequence determinations and analysis, genomic organization, expression analyis of pstS and phnD, overview
-
pstS expression analysis in wild-type strain R6, R6 muant strains, and diverse clinical isolates, overview
-
PT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression analysis, expression and complementation in yeast mutant PAM2
PT4, single copy gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression analysis, expression and complementation in yeast mutant PAM2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
1.3 kb promoter of TaPHT1.2 contains a number of phosphate-starvation responsive elements, including P1BS, WRKY-binding W-box, and helix-loop-helix-binding elements. Reporter gene expression analysis using this 1.3 kb promoter of TaPHT1.2 shows that the reporter gene is specifically induced by Pi-starvation and predominantly expressed in the roots
-
acute downregulation of renal Pi transporters in response to phosphate intake occurs also in the absence of parathyroid hormone and fibroblast growth factor 23 signalling
-
circadian expression of phosphate transporter gene pht4;1 is dependent on the protein circadian clock associated 1
-
during phosphate-stress conditions, isoforms PHT1:3 to PHT1:7, PHT3:1 to PHT3:4 and six members of PHO family show differential expression patterns between leaf and root tissues
-
enzyme expression is triggered in phosphate-starved mycorrhizal roots
expression is induced by phosphate starvation and light exposure
-
expression of isoform PT13 is induced by 0.01 and 0.1 mM As(V) treatment, while isoforms PT5 and PT10 are induced only by 0.1 mM As(V)
-
expression of Pht1:3 in Arabidopsis thaliana transgenic lines is significantly higher under low phosphate supply than that under normal phosphate supply
in gametophytes, Pht1;3 transcript levels increase in response to phosphate deficiency and arsenate exposure
-
in roots, expression of isoform PT2 and PT3 is strongly inhibited by 0.01 and 0.1 mM As(V) treatment
-
is induced by phosphate limitation
OsPT1 expression is strongly enhanced by mutation of phosphate over-accumulator 2, OsPHO2, but not by phosphate starvation response 2, OsPHR2. OsPT8 and OsPT4 are significantly and moderately upregulated, respectively, in OsPT1 overexpressing plants
-
PHO84, encoding the high-affinity H+/phosphate symporter, expression is activated by the Pho4 transcription factor in phosphate-starved cells as part of the PHO signalling pathway
Pht1;9 is induced by phosphate starvation
the Pht1;4 induction by phosphate deficiency is dependent on the existence of sugar
-
transcript level of PHT1:4 significantly increases both in roots and leaves under phosphate deficient conditions
-
transcription of PHO87 and PHO90, encoding the low-affinity H+/phosphate transport system, is independent of phosphate status
transcription of PT8 is suppressed after infection with rice pathogens and treatment with pathogen-associated molecular patterns
transcripts from operon PstSCAB are among the most abundant when the bacterium is grown in chickens or human cells
PHO84, encoding the high-affinity H+/phosphate symporter, expression is activated by the Pho4 transcription factor in phosphate-starved cells as part of the PHO signalling pathway
-
PHO84, encoding the high-affinity H+/phosphate symporter, expression is activated by the Pho4 transcription factor in phosphate-starved cells as part of the PHO signalling pathway
-
-
transcription of PHO87 and PHO90, encoding the low-affinity H+/phosphate transport system, is independent of phosphate status
-
transcription of PHO87 and PHO90, encoding the low-affinity H+/phosphate transport system, is independent of phosphate status
-
-
transcripts from operon PstSCAB are among the most abundant when the bacterium is grown in chickens or human cells
-
transcripts from operon PstSCAB are among the most abundant when the bacterium is grown in chickens or human cells
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
medicine
dysfunction causes numerous diseases, most reported clinical mutations lie in the transport domains, with a few occupying the dimerization or S domains
medicine
-
acute downregulation of renal Pi transporters in response to phosphate intake occurs also in the absence of parathyroid hormone and fibroblast growth factor 23 signalling. When fibroblast growth factor 23 signalling is blocked, a partial contribution of parathyroid hormone is revealed. Hexakisphosphate IP6 kinases are not involved. Acute phosphate intake does not alter PTH-related peptide and dopamine level
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Novak, R.; Cauwels, A.; Charpentier, E.; Tuomanen, E.
Identification of a Streptococcus pneumoniae gene locus encoding proteins of an ABC phosphate transporter and a two-component regulatory system
J. Bacteriol.
181
1126-1133
1999
Streptococcus pneumoniae
brenda
Bhatt, K.; Banerjee, S.K.; Chakraborti, P.K.
Evidence that phosphate specific transporter is amplified in a fluoroquinoline resistant Mycobacterium smegmatis
Eur. J. Biochem.
267
4028-4032
2000
Mycolicibacterium smegmatis
brenda
Braibant, M.; Lefevre, P.; de Wit, L.; Ooms, J.; Peirs, P.; Huygen, K.; Wattiez, R.; Content, J.
Identification of a second Mycobacterium tuberculosis gene cluster encoding protein of an ABC phosphate transporter
FEBS Lett.
394
206-212
1996
Mycobacterium tuberculosis
brenda
Chan, F.Y.; Torriani, A.
PstB protein of the phosphate-specific transport system of Escherichia coli is an ATPase
J. Bacteriol.
178
3974-3977
1996
Escherichia coli
brenda
Braibant, M.; Lefevre, P.; de Wit, L.; Peirs, P.; Ooms, J.; Huygen, K.; Andersen, A.B.; Content, J.
A Mycobacterium tuberculosis gene cluster encoding proteins of a phosphate transporter homologous to the Escherichia coli Pst system
Gene
176
171-176
1996
Mycobacterium tuberculosis
brenda
Webb, D.C.; Rosenberg, H.; Cox, G.B.
Mutational analysis of the Escherichia coli phosphate-specific transport sytem, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices
J. Biol. Chem.
267
24661-24668
1992
Escherichia coli
brenda
Chakraborti, P.K.; Bhatt, K.; Banerjeee, S.K.; Misra, P.
Role of an ABC importer in mycobacterial drug resistance
Biosci. Rep.
19
293-300
1999
Mycolicibacterium smegmatis
brenda
Cox, G.B.; Webb, D.; Godovac-Zimmermann, J.; Rosenberg, H.; Rosenberg, H.
Specific amino acid residues in both the PstB and PstC proteins are required for phosphate transport by the Escherichia coli pst system
J. Bacteriol.
171
1531-1534
1989
Escherichia coli
brenda
Sarin, J.; Aggarwal, S.; Chaba, R.; Varshney, G.C.; Chakraborti, P.K.
B-subunit of phosphate-specific transporter from Mycobacterium tuberculosis is a thermostable ATPase
J. Biol. Chem.
276
44590-44597
2001
Mycobacterium tuberculosis, Escherichia coli, Mycobacterium tuberculosis H37Rv
brenda
Allenby, N.E.; O'Connor, N.; Pragai, Z.; Carter, N.M.; Miethke, M.; Engelmann, S.; Hecker, M.; Wipat, A.; Ward, A.C.; Harwood, C.R.
Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter
Microbiology
150
2619-2628
2004
Bacillus subtilis
brenda
Vyas, N.K.; Vyas, M.N.; Quiocho, F.A.
Crystal structure of M tuberculosis ABC phosphate transport receptor: specificity and charge compensation dominated by ion-dipole interactions
Structure
11
765-774
2003
Mycobacterium tuberculosis
brenda
Gupta, S.; Chakraborti, P.K.; Sarkar, D.
Nucleotide-induced conformational change in the catalytic subunit of the phosphate-specific transporter from M. tuberculosis: implications for the ATPase structure
Biochim. Biophys. Acta
1750
112-121
2005
Mycobacterium tuberculosis
brenda
Leung, J.C.; Barac-Nieto, M.; Hering-Smith, K.; Silverstein, D.M.
Expression of the rat renal PiT-2 phosphate transporter
Horm. Metab. Res.
37
265-269
2005
Rattus norvegicus, Didelphis virginiana, Canis lupus familiaris
brenda
Yuan, Z.C.; Zaheer, R.; Finan, T.M.
Regulation and properties of PstSCAB, a high-affinity, high-velocity phosphate transport system of Sinorhizobium meliloti
J. Bacteriol.
188
1089-1102
2006
Sinorhizobium meliloti
brenda
Fischer, R.J.; Oehmcke, S.; Meyer, U.; Mix, M.; Schwarz, K.; Fiedler, T.; Bahl, H.
Transcription of the pst operon of Clostridium acetobutylicum is dependent on phosphate concentration and pH
J. Bacteriol.
188
5469-5478
2006
Clostridium acetobutylicum
brenda
Gebhard Susann, G.S.; Tran Sieu , T.S.; Cook Gregory , C.G.
The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate
Microbiology
152
3453-3465
2006
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis SG34
brenda
Soualhine, H.; Brochu, V.; Menard, F.; Papadopoulou, B.; Weiss, K.; Bergeron, M.G.; Legare, D.; Drummelsmith, J.; Ouellette, M.
A proteomic analysis of penicillin resistance in Streptococcus pneumoniae reveals a novel role for PstS, a subunit of the phosphate ABC transporter
Mol. Microbiol.
58
1430-1440
2005
Streptococcus pneumoniae
brenda
Hase, A.; Nishikoori, M.; Okuyama, H.
Induction of high affinity phosphate transporter in the duckweed Spirodela oligorrhiza
Physiol. Plant.
120
271-279
2004
Landoltia punctata
brenda
Gonzalez, E.; Solano, R.; Rubio, V.; Leyva, A.; Paz-Ares, J.
Phosphate transporter traffic facilitator1 is a plant-specific SEC12-related protein that enables the endoplasmic reticulum exit of a high-affinity phosphate transporter in Arabidopsis
Plant Cell
17
3500-3512
2005
Arabidopsis thaliana
brenda
Nagy, R.; Karandashov, V.; Chague, V.; Kalinkevich, K.; Tamasloukht, M.; Xu, G.; Jakobsen, I.; Levy, A.A.; Amrhein, N.; Bucher, M.
The characterization of novel mycorrhiza-specific phosphate transporters from Lycopersicon esculentum and Solanum tuberosum uncovers functional redundancy in symbiotic phosphate transport in solanaceous species
Plant J.
42
236-250
2005
Solanum tuberosum (Q563I1), Solanum tuberosum, Solanum lycopersicum (Q563I3), Solanum lycopersicum
brenda
Li, X.; Yang, H.Y.; Giachelli, C.M.
Role of the sodium-dependent phosphate cotransporter, Pit-1, in vascular smooth muscle cell calcification
Circ. Res.
98
905-912
2006
Homo sapiens
brenda
Jacobsen, S.M.; Lane, M.C.; Harro, J.M.; Shirtliff, M.E.; Mobley, H.L.
The high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection
FEMS Immunol. Med. Microbiol.
52
180-193
2008
Proteus mirabilis, Proteus mirabilis HI4320
brenda
Nagy, R.; Vasconcelos, M.J.; Zhao, S.; McElver, J.; Bruce, W.; Amrhein, N.; Raghothama, K.G.; Bucher, M.
Differential regulation of five Pht1 phosphate transporters from maize (Zea mays L.)
Plant Biol.
8
186-197
2006
Zea mays (Q49B46), Zea mays (Q49B45), Zea mays (Q49B44), Zea mays (Q49B43), Zea mays (Q49B42), Zea mays (Q5CC71)
brenda
Xiao, K.; Liu, J.; Dewbre, G.; Harrison, M.; Wang, Z.Y.
Isolation and characterization of root-specific phosphate transporter promoters from Medicago truncatula
Plant Biol.
8
439-449
2006
Medicago truncatula (O22301), Medicago truncatula (O22302), Medicago truncatula
brenda
Catarecha, P.; Segura, M.D.; Franco-Zorrilla, J.M.; Garcia-Ponce, B.; Lanza, M.; Solano, R.; Paz-Ares, J.; Leyva, A.
A mutant of the Arabidopsis phosphate transporter PHT1;1 displays enhanced arsenic accumulation
Plant Cell
19
1123-1133
2007
Arabidopsis thaliana, Saccharomyces cerevisiae
brenda
Ghoshal, A.; Mukhopadhyay, S.; Demine, R.; Forgber, M.; Jarmalavicius, S.; Saha, B.; Sundar, S.; Walden, P.; Mandal, C.; Mandal, C.
Detection and characterization of a sialoglycosylated bacterial ABC-type phosphate transporter protein from patients with visceral leishmaniasis
Glycoconj. J.
26
675-689
2009
Pseudomonas aeruginosa
brenda
Pavon, L.R.; Lundh, F.; Lundin, B.; Mishra, A.; Persson, B.L.; Spetea, C.
Arabidopsis ANTR1 is a thylakoid Na+-dependent phosphate transporter: functional characterization in Escherichia coli
J. Biol. Chem.
283
13520-13527
2008
Arabidopsis thaliana (O82390), Arabidopsis thaliana
brenda
O'May, G.A.; Jacobsen, S.M.; Longwell, M.; Stoodley, P.; Mobley, H.L.; Shirtliff, M.E.
The high-affinity phosphate transporter Pst in Proteus mirabilis HI4320 and its importance in biofilm formation
Microbiology
155
1523-1535
2009
Proteus mirabilis, Proteus mirabilis HI4320
brenda
Wegmueller, S.; Svistoonoff, S.; Reinhardt, D.; Stuurman, J.; Amrhein, N.; Bucher, M.
A transgenic dTph1 insertional mutagenesis system for forward genetics in mycorrhizal phosphate transport of Petunia
Plant J.
54
1115-1127
2008
Petunia x hybrida
brenda
Ruiz-Pavon, L.; Karlsson, P.M.; Carlsson, J.; Samyn, D.; Persson, B.; Persson, B.L.; Spetea, C.
Functionally important amino acids in the Arabidopsis thylakoid phosphate transporter: homology modeling and site-directed mutagenesis
Biochemistry
49
6430-6439
2010
Arabidopsis thaliana
brenda
Huerlimann, H.C.; Pinson, B.; Stadler-Waibel, M.; Zeeman, S.C.; Freimoser, F.M.
The SPX domain of the yeast low-affinity phosphate transporter Pho90 regulates transport activity
EMBO Rep.
10
1003-1008
2009
Saccharomyces cerevisiae
brenda
Weinman, E.J.; Steplock, D.; Zhang, Y.; Biswas, R.; Bloch, R.J.; Shenolikar, S.
Cooperativity between the phosphorylation of Thr95 and Ser77 of NHERF-1 in the hormonal regulation of renal phosphate transport
J. Biol. Chem.
285
25134-25138
2010
Homo sapiens
brenda
Iharada, M.; Miyaji, T.; Fujimoto, T.; Hiasa, M.; Anzai, N.; Omote, H.; Moriyama, Y.
Type 1 sodium-dependent phosphate transporter (SLC17A1 Protein) is a Cl(-)-dependent urate exporter
J. Biol. Chem.
285
26107-26113
2010
Mus musculus
brenda
Yadav, V.; Kumar, M.; Deep, D.K.; Kumar, H.; Sharma, R.; Tripathi, T.; Tuteja, N.; Saxena, A.K.; Johri, A.K.
A phosphate transporter from the root endophytic fungus Piriformospora indica plays a role in phosphate transport to the host plant
J. Biol. Chem.
285
26532-26544
2010
Serendipita indica (A8N031), Serendipita indica
brenda
Lazard, M.; Blanquet, S.; Fisicaro, P.; Labarraque, G.; Plateau, P.
Uptake of selenite by Saccharomyces cerevisiae involves the high- and low-affinity orthophosphate transporters
J. Biol. Chem.
285
32029-32037
2011
Saccharomyces cerevisiae (P25297), Saccharomyces cerevisiae (P25360), Saccharomyces cerevisiae (P39535), Saccharomyces cerevisiae (P27514), Saccharomyces cerevisiae
brenda
Miao, J.; Sun, J.; Liu, D.; Li, B.; Zhang, A.; Li, Z.; Tong, Y.
Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties
J. Genet. Genomics
36
455-466
2009
Triticum sp.
brenda
Cesselin, B.; Ali, D.; Gratadoux, J.J.; Gaudu, P.; Duwat, P.; Gruss, A.; El Karoui, M.
Inactivation of the Lactococcus lactis high-affinity phosphate transporter confers oxygen and thiol resistance and alters metal homeostasis
Microbiology
155
2274-2281
2009
Lactococcus lactis
brenda
Preuss, C.P.; Huang, C.Y.; Gilliham, M.; Tyerman, S.D.
Channel-like characteristics of the low-affinity barley phosphate transporter PHT1;6 when expressed in Xenopus oocytes
Plant Physiol.
152
1431-1441
2010
Hordeum vulgare
brenda
Cowan, C.M.; Zhang, X.; James, A.W.; Mari Kim, T.; Sun, N.; Wu, B.; Ting, K.; Soo, C.
NELL-1 increases pre-osteoblast mineralization using both phosphate transporter Pit1 and Pit2
Biochem. Biophys. Res. Commun.
422
351-357
2012
Mus musculus
brenda
Ghillebert, R.; Swinnen, E.; De Snijder, P.; Smets, B.; Winderickx, J.
Differential roles for the low-affinity phosphate transporters Pho87 and Pho90 in Saccharomyces cerevisiae
Biochem. J.
434
243-251
2011
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
brenda
Byskov, K.; Jensen, N.; Kongsfelt, I.; Wielsoe, M.; Pedersen, L.; Haldrup, C.; Pedersen, L.
Regulation of cell proliferation and cell density by the inorganic phosphate transporter PiT1
Cell Div.
7
7
2012
Homo sapiens (Q8WUM9), Homo sapiens, Mus musculus (Q61609), Mus musculus
brenda
Nussaume, L.; Kanno, S.; Javot, H.; Marin, E.; Pochon, N.; Ayadi, A.; Nakanishi, T.M.; Thibaud, M.C.
Phosphate import in plants: focus on the PHT1 transporters
Front. Plant Sci.
2
83
2011
Arabidopsis thaliana, Saccharomyces cerevisiae, Neurospora crassa, Diversispora versiformis, Medicago truncatula, Hordeum vulgare, Solanum tuberosum, Oryza sativa, Solanum lycopersicum
brenda
Wang, G.Y.; Shi, J.L.; Ng, G.; Battle, S.L.; Zhang, C.; Lu, H.
Circadian clock-regulated phosphate transporter PHT4;1 plays an important role in Arabidopsis defense
Mol. Plant
4
516-526
2011
Arabidopsis thaliana, Arabidopsis thaliana Col-0
brenda
Remy, E.; Cabrito, T.R.; Batista, R.A.; Teixeira, M.C.; Sa-Correia, I.; Duque, P.
The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation
New Phytol.
195
356-371
2012
Arabidopsis thaliana, Arabidopsis thaliana Col-0
brenda
Preuss, C.P.; Huang, C.Y.; Tyerman, S.D.
Proton-coupled high-affinity phosphate transport revealed from heterologous characterization in Xenopus of barley-root plasma membrane transporter, HvPHT1;1
Plant Cell Environ.
34
681-689
2011
Hordeum vulgare (Q8H6E0), Hordeum vulgare
brenda
Nagarajan, V.K.; Jain, A.; Poling, M.D.; Lewis, A.J.; Raghothama, K.G.; Smith, A.P.
Arabidopsis Pht1;5 mobilizes phosphate between source and sink organs and influences the interaction between phosphate homeostasis and ethylene signaling
Plant Physiol.
156
1149-1163
2011
Arabidopsis thaliana
brenda
Irigoyen, S.; Karlsson, P.M.; Kuruvilla, J.; Spetea, C.; Versaw, W.K.
The sink-specific plastidic phosphate transporter PHT4;2 influences starch accumulation and leaf size in Arabidopsis
Plant Physiol.
157
1765-1777
2011
Arabidopsis thaliana (Q7XJR2)
brenda
Sun, S.; Gu, M.; Cao, Y.; Huang, X.; Zhang, X.; Ai, P.; Zhao, J.; Fan, X.; Xu, G.
A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in Pi-replete rice
Plant Physiol.
159
1571-1581
2012
Oryza sativa
brenda
Wu, Z.; Zhao, J.; Gao, R.; Hu, G.; Gai, J.; Xu, G.; Xing, H.
Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max
PLoS ONE
6
e19752
2011
Glycine max (F5A0U7), Glycine max (C0LZ80), Glycine max gantai (F5A0U7), Glycine max gantai (C0LZ80)
brenda
Pumplin, N.; Zhang, X.; Noar, R.D.; Harrison, M.J.
Polar localization of a symbiosis-specific phosphate transporter is mediated by a transient reorientation of secretion
Proc. Natl. Acad. Sci. USA
109
E665-E672
2012
Medicago truncatula, Medicago truncatula A17
brenda
Liu, P.; Chen, S.; Song, A.; Zhao, S.; Fang, W.; Guan, Z.; Liao, Y.; Jiang, J.; Chen, F.
A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum
BMC Plant Biol.
14
18
2014
Chrysanthemum x morifolium
brenda
Lapis-Gaza, H.R.; Jost, R.; Finnegan, P.M.
Arabidopsis phosphate transporter1 genes PHT1;8 and PHT1;9 are involved in root-to-shoot translocation of orthophosphate
BMC Plant Biol.
14
334
2014
Arabidopsis thaliana
brenda
Wang, G.; Zhang, C.; Battle, S.; Lu, H.
The phosphate transporter PHT4;1 is a salicylic acid regulator likely controlled by the circadian clock protein CCA1
Front. Plant Sci.
5
701
2014
Arabidopsis thaliana
brenda
Chen, G.; Yan, W.; Yang, S.; Wang, A.; Gai, J.; Zhu, Y.
Overexpression of rice phosphate transporter gene OsPT2 enhances tolerance to low phosphorus stress in soybean
J. Agric. Sci. Technol.
17
469-482
2015
Oryza sativa
-
brenda
Park, Y.; Bang, I.
Bacterial phosphate homeostasis: Role of phosphate transporters
Korean J. Microbiol.
48
57-65
2012
Myxococcus xanthus, Escherichia coli, Mycolicibacterium smegmatis, Arthrobacter sp., Rhodobacter capsulatus, Pseudomonas aeruginosa
-
brenda
Xie, X.; Huang, W.; Liu, F.; Tang, N.; Liu, Y.; Lin, H.; Zhao, B.
Functional analysis of the novel mycorrhiza-specific phosphate transporter AsPT1 and PHT1 family from Astragalus sinicus during the arbuscular mycorrhizal symbiosis
New Phytol.
198
836-852
2013
Astragalus sinicus (K0IIN5), Astragalus sinicus (K0I2D6), Astragalus sinicus
brenda
DiTusa, S.F.; Fontenot, E.B.; Wallace, R.W.; Silvers, M.A.; Steele, T.N.; Elnagar, A.H.; Dearman, K.M.; Smith, A.P.
A member of the phosphate transporter 1 (Pht1) family from the arsenic-hyperaccumulating fern Pteris vittata is a high-affinity arsenate transporter
New Phytol.
209
762-772
2016
Pteris vittata
brenda
Wang, X.; Wang, Y.; Pineros, M.A.; Wang, Z.; Wang, W.; Li, C.; Wu, Z.; Kochian, L.V.; Wu, P.
Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in rice
Plant Cell Environ.
37
1159-1170
2014
Oryza sativa
brenda
Cardona-Lopez, X.; Cuyas, L.; Marin, E.; Rajulu, C.; Irigoyen, M.L.; Gil, E.; Puga, M.I.; Bligny, R.; Nussaume, L.; Geldner, N.; Paz-Ares, J.; Rubio, V.
ESCRT-III-Associated Protein ALIX mediates high affinity phosphate transporter trafficking to maintain phosphate homeostasis in Arabidopsis
Plant Cell
27
2560-2581
2015
Arabidopsis thaliana
brenda
Muehe, E.; Eisele, J.; Daus, B.; Kappler, A.; Harter, K.; Chaban, C.
Are rice (Oryza sativa L.) phosphate transporters regulated similarly by phosphate and arsenate? A comprehensive study
Plant Mol. Biol.
85
301-316
2014
Oryza sativa
brenda
Ren, F.; Zhao, C.Z.; Liu, C.S.; Huang, K.L.; Guo, Q.Q.; Chang, L.L.; Xiong, H.; Li, X.B.
A Brassica napus PHT1 phosphate transporter, BnPht1;4, promotes phosphate uptake and affects roots architecture of transgenic Arabidopsis
Plant Mol. Biol.
86
595-607
2014
Brassica napus
brenda
Qin, L.; Zhao, J.; Tian, J.; Chen, L.; Sun, Z.; Guo, Y.; Lu, X.; Gu, M.; Xu, G.; Liao, H.
The high-affinity phosphate transporter GmPT5 regulates phosphate transport to nodules and nodulation in soybean
Plant Physiol.
159
1634-1643
2012
Glycine max (C3UZD2), Glycine max
brenda
Jia, F.; Wan, X.; Zhu, W.; Sun, D.; Zheng, C.; Liu, P.; Huang, J.
Overexpression of mitochondrial phosphate transporter 3 severely hampers plant development through regulating mitochondrial function in Arabidopsis
PLoS ONE
10
e0129717
2015
Arabidopsis thaliana (Q9FMU6)
brenda
Gonzalez, D.; Richez, M.; Bergonzi, C.; Chabriere, E.; Elias, M.
Crystal structure of the phosphate-binding protein (PBP-1) of an ABC-type phosphate transporter from Clostridium perfringens
Sci. Rep.
4
6636
2014
Clostridium perfringens (A0A0H2YSI2), Clostridium perfringens
brenda
Penaloza, E.; Santiago, M.; Cabrera, S.; Munoz, G.; Corcuera, L.; Silva, H.
Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat
Biol. Plant.
61
453-462
2017
Arabidopsis thaliana
-
brenda
Karlsson, P.M.; Herdean, A.; Adolfsson, L.; Beebo, A.; Nziengui, H.; Irigoyen, S.; Uennep, R.; Zsiros, O.; Nagy, G.; Garab, G.; Aronsson, H.; Versaw, W.K.; Spetea, C.
The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth
Plant J.
84
99-110
2015
Arabidopsis thaliana (O82390)
brenda
Daryadel, A.; Haykir, B.; Kueng, C.; Bugarski, M.; Bettoni, C.; Schnitzbauer, U.; Hernando, N.; Hall, A.; Wagner, C.
Acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals
Acta Physiol. (Oxf.)
235
e13815
2022
Mus musculus
brenda
Yang, S.; Feng, Y.; Zhao, Y.; Bai, J.; Wang, J.
Overexpression of a Eutrema salsugineum phosphate transporter gene EsPHT1;4 enhances tolerance to low phosphorus stress in soybean
Biotechnol. Lett.
42
2425-2439
2020
Eutrema salsugineum
brenda
Vuppada, R.; Hansen, C.; Strickland, K.; Kelly, K.; McCleary, W.
Phosphate signaling through alternate conformations of the PstSCAB phosphate transporter
BMC Microbiol.
18
008
2018
Escherichia coli, Escherichia coli BW25113
brenda
Zhu, X.; Boulet, A.; Buckley, K.; Phillips, C.; Gammon, M.; Oldfather, L.; Moore, S.; Leary, S.; Cobine, P.
Mitochondrial copper and phosphate transporter specificity was defined early in the evolution of eukaryotes
eLife
10
1-65
2021
Mus musculus (Q8VEM8)
brenda
Ahmad, I.; Rawoof, A.; Islam, K.; Momo, J.; Ramchiary, N.
Identification and expression analysis of phosphate transporter genes and metabolites in response to phosphate stress in Capsicum annuum
Environ. Exp. Bot.
190
104597
2021
Capsicum annuum
-
brenda
Liao, Y.; Li, J.; Pan, R.; Chiou, T.
Structur-function analysis reveals amino acid residues of Arabidopsis phosphate transporter AtPHT1;1 crucial for its activity
Front. Plant Sci.
10
1158
2019
Arabidopsis thaliana (Q8VYM2)
brenda
Kelliher, J.; Radin, J.; Grim, K.; Solorzano, P.; Degnan, P.; Kehl-Fie, T.
Acquisition of the phosphate transporter NptA enhances Staphylococcus aureus pathogenesis by improving phosphate uptake in divergent environments
Infect. Immun.
86
e00631-37
2018
Staphylococcus aureus (A0A290QKL9), Staphylococcus aureus Newman (A0A290QKL9)
brenda
Sinha, R.; LeVeque, R.; Bowlin, M.; Gray, M.; DiRita, V.
Phosphate transporter PstSCAB of Campylobacter jejuni is a critical determinant of lactate-dependent growth and colonization in chickens
J. Bacteriol.
202
e00716-19
2020
Campylobacter jejuni, Campylobacter jejuni 81-176
brenda
Zhou, J.; Lu, M.; Zhang, C.; Qu, X.; Liu, Y.; Yang, J.; Yuan, J.
Isolation and functional characterisation of the PHT1 gene encoding a high-affinity phosphate transporter in Camellia oleifera
J. Hortic. Sci. Biotechnol.
95
553-564
2020
Camellia oleifera (W8QHB6)
-
brenda
Yu, G.; Huang, S.; He, R.; Li, Y.; Cheng, X.
Transgenic rice overexperessing a tomato mitochondrial phosphate transporter, SlMPT3;1, promotes phosphate uptake and increases grain yield
J. Plant Biol.
61
383-400
2018
Solanum lycopersicum (K4BDP2)
-
brenda
Sun, T.; Zhou, B.; Pei, T.; Meng, H.; Zhang, J.; Ma, F.; Wei, Q.
Phosphate transporter PHT1;7 enhances phosphorus accumulation and improves low phosphorus and drought tolerance
J. Plant Biol.
64
403-416
2021
Malus domestica (A0A6B9RIE8)
-
brenda
Montenegro, R.; Vieto, S.; Wicki-Emmenegger, D.; Vasquez-Castro, F.; Coronado-Ruiz, C.; Fuentes-Schweizer, P.; Calderon, P.; Pereira, R.; Chavarrixada, M.
The putative phosphate transporter PitB (Pp1373) is involved in tellurite uptake in Pseudomonas putida KT2440
Microbiology
167
0000
2021
Pseudomonas putida (Q88N43)
brenda
Luan, M.; Liu, J.; Liu, Y.; Han, X.; Sun, G.; Lan, W.; Luan, S.
Vacuolar phosphate transporter 1 (VPT1) affects arsenate tolerance by regulating phosphate homeostasis in Arabidopsis
Plant Cell Physiol.
59
1345-1352
2018
Arabidopsis thaliana
brenda
Liu, F.; Xu, Y.; Han, G.; Wang, W.; Li, X.; Cheng, B.
Identification and functional characterization of a maize phosphate transporter induced by mycorrhiza formation
Plant Cell Physiol.
59
1683-1694
2018
Zea mays
brenda
Yang, Z.; Yang, J.; Wang, Y.; Wang, F.; Mao, W.; He, Q.; Xu, J.; Wu, Z.; Mao, C.
Protein phosphatase95 regulates phosphate homeostasis by affecting phosphate transporter trafficking in rice
Plant Cell
32
740-757
2020
Oryza sativa Japonica Group
brenda
Liu, X.L.; Wang, L.; Wang, X.W.; Yan, Y.; Yang, X.L.; Xie, M.Y.; Hu, Z.; Shen, X.; Ai, H.; Lin, H.H.; Xu, G.H.; Yang, J.; Sun, S.B.
Mutation of the chloroplast-localized phosphate transporter OsPHT2;1 reduces flavonoid accumulation and UV tolerance in rice
Plant J.
102
53-67
2020
Oryza sativa Japonica Group
brenda
Amna, S.; Qamar, S.; Turab Naqvi, A.; Al-Huqail, A.; Qureshi, M.
Role of sulfur in combating arsenic stress through upregulation of important proteins, and in-silico analysis to study the interaction between phosphate transporter (PHO1), arsenic and phosphate in spinach
Plant Physiol. Biochem.
157
348-358
2020
Spinacia oleracea (A0A0K9RUE0)
brenda
Luan, M.; Zhao, F.; Han, X.; Sun, G.; Yang, Y.; Liu, J.; Shi, J.; Fu, A.; Lan, W.; Luan, S.
Vacuolar phosphate transporters contribute to systemic phosphate homeostasis vital for reproductive development in Arabidopsis 1
Plant Physiol.
179
640-655
2019
Arabidopsis thaliana
brenda
Cao, M.; Liu, H.; Zhang, C.; Wang, D.; Liu, X.; Chen, Q.
Functional analysis of stpht1;7, a Solanum tuberosum L. phosphate transporter gene, in growth and drought tolerance
Plants (Basel)
9
1384
2020
Solanum tuberosum
brenda
Yang, W.; Baek, D.; Yun, D.; Lee, K.; Hong, S.; Bae, K.; Chung, Y.; Kwon, Y.; Kim, D.; Jung, K.; Kim, D.
Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter
PLoS ONE
13
e0194628
2018
Oryza sativa, Oryza sativa Dongjin
brenda
Liu, Y.; Li, C.; Gupta, M.; Verma, N.; Johri, A.; Stroud, R.; Voth, G.
Key computational findings reveal proton transfer as driving the functional cycle in the phosphate transporter PiPT
Proc. Natl. Acad. Sci. USA
118
e2101932118
2021
Serendipita indica (G4TS85), Serendipita indica DSM 11827 (G4TS85)
brenda
Ruili, L.; Jiaoling, W.; Lei, X.; Meihao, S.; Keke, Y.; Hongyu, Z.
Functional analysis of phosphate transporter OsPHT4 family members in rice
Rice Sci.
27
493-503
2020
Oryza sativa Japonica Group, Oryza sativa Japonica Group Shishoubaimao
-
brenda
Tsai, J.; Chu, C.; Lin, M.; Chou, Y.; Hong, R.; Yen, C.; Hsiao, C.; Sun, Y.
Structure of the sodium-dependent phosphate transporter reveals insights into human solute carrier SLC20
Sci. Adv.
6
eabb4024
2020
Thermotoga maritima (Q9WY99), Homo sapiens (Q08357), Thermotoga maritima DSM 3109 (Q9WY99)
brenda
Dong, Z.; Li, W.; Liu, J.; Li, L.; Pan, S.; Liu, S.; Gao, J.; Liu, L.; Liu, X.; Wang, G.; Dai, L.
The rice phosphate transporter protein OsPT8 regulates disease resistance and plant growth
Sci. Rep.
9
5408
2019
Oryza sativa Japonica Group (Q8H6G8)
brenda
EXTERNAL LINKS (specific for EC-Number 7.3.2.1)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
