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Information on EC 6.3.2.9 - UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase and Organism(s) Pseudomonas aeruginosa

for references in articles please use BRENDA:EC6.3.2.9

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IUBMB Comments

Involved in the synthesis of a cell-wall peptide in bacteria.

The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

Synonyms
murd ligase, d-glutamic acid-adding enzyme, udp-n-acetylmuramoyl-l-alanine:d-glutamate ligase, udp-n-acetylmuramoyl-l-alanyl-d-glutamate synthetase, udp-n-acetylmuramoyl-l-alanine-d-glutamate ligase, d-glutamate-adding enzyme, more

SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-Glutamate-adding enzyme
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D-glutamic acid adding enzyme
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D-Glutamic acid-adding enzyme
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-
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MurD cell wall enzyme
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Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamate
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-
-
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UDP-Mur-Nac-L-Ala:L-Glu ligase
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-
-
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UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
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-
-
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UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
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-
-
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UDPMurNAc-L-alanine:D-glutamate ligase (ADP-forming)
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-
-
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Uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligase
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-
-
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Uridine diphospho-N-acetylmuramoylalanyl-D-glutamate synthetase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxamide formation
-
-
-
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carboxylic acid amide formation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
MetaCyc
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing), UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (ADP-forming)
Involved in the synthesis of a cell-wall peptide in bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-59-0
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
HLPTSSLFDTTG
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-
RPTHSPI
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peptides are synthesized from consensus sequences to evaluate their inhibitory potential against the essential MurD enzyme. The C-7-C mers MurDp1 (RPTHSPI) gives a decrease of MurD ATPase activity with a significant IC50 value of 4 mM. The 12 mers MurDp2 (HLPTSSLFDTTG) inhibits MurD with an IC50 value of 15 mM indicating a weak inhibition. Both peptides show a correlation between an increase in concentration versus an increase in inhibition values
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15
HLPTSSLFDTTG
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-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15
RPTHSPI
Pseudomonas aeruginosa
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peptides are synthesized from consensus sequences to evaluate their inhibitory potential against the essential MurD enzyme. The C-7-C mers MurDp1 (RPTHSPI) gives a decrease of MurD ATPase activity with a significant IC50 value of 4 mM. The 12 mers MurDp2
additional information
additional information
Pseudomonas aeruginosa
-
peptides are synthesized from consensus sequences to evaluate their inhibitory potential against the essential MurD enzyme. The C-7-C mers MurDp1 gives a decrease of MurD ATPase activity with a significant IC50 value of 4 mM. The 12 mers MurDp2 inhibits MurD with an IC50 value of 15 mM
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top print hide Go to Organism Search
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line Links Gene Links
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A8G7CGF6_PSEAI
448
0
48112
TrEMBL
-
A0A2R3ISC7_PSEAI
448
0
47967
TrEMBL
-
A0A8G7SGT0_PSEAI
448
0
48094
TrEMBL
-
A0A8G3AU10_PSEAI
448
0
48090
TrEMBL
-
A0A080VV57_PSEAI
448
0
48152
TrEMBL
-
A0A8B5BDE9_PSEAI
448
0
48094
TrEMBL
-
A0A367MFH4_PSEAI
448
0
48110
TrEMBL
-
A0A8G3KCS8_PSEAI
448
0
48138
TrEMBL
-
A0A7M3AVR4_PSEAI
448
0
48094
TrEMBL
-
A0A8G6HX35_PSEAI
448
0
48096
TrEMBL
-
A0A8G6XL77_PSEAI
448
0
48106
TrEMBL
-
A0A8G7D244_PSEAI
448
0
48079
TrEMBL
-
A0A8G7PTE4_PSEAI
448
0
48108
TrEMBL
-
A0A4P0TDH8_PSEAI
848
9
91857
TrEMBL
-
A0A0C7CYE6_PSEAI
448
0
48080
TrEMBL
-
A0A3S0IXC4_PSEAI
448
0
48049
TrEMBL
-
A0A8G4DND0_PSEAI
448
0
48052
TrEMBL
-
A0A8G4J0H5_PSEAI
448
0
48182
TrEMBL
-
A0A8G2YKL1_PSEAI
448
0
48092
TrEMBL
-
A0A4U9R7F6_PSEAI
448
0
48066
TrEMBL
-
A0A8G2S159_PSEAI
448
0
48082
TrEMBL
-
A0A5E5R0B1_PSEAI
448
0
48108
TrEMBL
-
A0A6A9K1Y6_PSEAI
448
0
48050
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51000
-
x * 51000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 51000, SDS-PAGE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Paradis-Bleau, C.; Beaumont, M.; Boudreault, L.; Lloyd, A.; Sanschagrin, F.; Bugg, T.D.; Levesque, R.C.
Selection of peptide inhibitors against the Pseudomonas aeruginosa MurD cell wall enzyme
Peptides
27
1693-1700
2006
Pseudomonas aeruginosa
Manually annotated by BRENDA team