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Information on EC 6.2.1.53 - L-proline-[L-prolyl-carrier protein] ligase
for references in articles please use BRENDA:EC6.2.1.53
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EC Tree 6 Ligases
6.2 Forming carbon-sulfur bonds
6.2.1 Acid-thiol ligases
6.2.1.53 L-proline-[L-prolyl-carrier protein] ligase
IUBMB Comments
The enzyme participates in the biosynthesis of several pyrrole-containing compounds, such as undecylprodigiosin, prodigiosin, pyoluteorin, and coumermycin A1. It catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4′-phosphopantheine moiety of an L-prolyl-carrier protein.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
show+
+
holo-[L-prolyl-carrier protein]
=
+
+
L-prolyl-[L-prolyl-carrier protein]
Synonyms
l-prolyl-amp ligase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
bmp4
-
-
-
-
L-prolyl-AMP ligase
pigl
-
-
-
-
pltF
SC3F7.11
pltF
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] = AMP + L-prolyl-[L-prolyl-carrier protein]
(1b)
-
-
-
ATP + L-proline + holo-[L-prolyl-carrier protein] = AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
overall reaction
-
-
-
ATP + L-proline = diphosphate + (L-prolyl)adenylate
(1a)
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
apratoxin A biosynthesis, brominated pyrroles biosynthesis, fumitremorgin C biosynthesis, malbrancheamide biosynthesis, prodigiosin biosynthesis, streptorubin B biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
L-proline:[L-prolyl-carrier protein] ligase (AMP-forming)
The enzyme participates in the biosynthesis of several pyrrole-containing compounds, such as undecylprodigiosin, prodigiosin, pyoluteorin, and coumermycin A1. It catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
Pseudomonas fluorescens ATCC BAA-4770
Substrates: -
Products: -
Products: -
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
ATP + L-proline
diphosphate + (L-prolyl)adenylate
Substrates: -
Products: -
Products: -
?
ATP + L-proline
diphosphate + (L-prolyl)adenylate
Pseudomonas fluorescens ATCC BAA-4770
Substrates: -
Products: -
Products: -
?
ATP + L-proline
diphosphate + (L-prolyl)adenylate
Substrates: -
Products: -
Products: -
?
ATP + L-proline
diphosphate + (L-prolyl)adenylate
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
Pseudomonas fluorescens ATCC BAA-4770
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
Pseudomonas fluorescens ATCC BAA-4770
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine
Products: -
Products: -
?
additional information
?
-
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate
Products: -
Products: -
?
additional information
?
-
Pseudomonas fluorescens ATCC BAA-4770
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine
Products: -
Products: -
?
additional information
?
-
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine
Products: -
Products: -
?
additional information
?
-
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate
Products: -
Products: -
?
additional information
?
-
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
Pseudomonas fluorescens ATCC BAA-4770
Substrates: -
Products: -
Products: -
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
AMP + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
ATP + L-proline
diphosphate + (L-prolyl)adenylate
Substrates: -
Products: -
Products: -
?
ATP + L-proline
diphosphate + (L-prolyl)adenylate
Pseudomonas fluorescens ATCC BAA-4770
Substrates: -
Products: -
Products: -
?
ATP + L-proline
diphosphate + (L-prolyl)adenylate
Substrates: -
Products: -
Products: -
?
ATP + L-proline
diphosphate + (L-prolyl)adenylate
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
Pseudomonas fluorescens ATCC BAA-4770
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine
Products: -
Products: -
?
additional information
?
-
Pseudomonas fluorescens ATCC BAA-4770
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine
Products: -
Products: -
?
additional information
?
-
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine
Products: -
Products: -
?
additional information
?
-
Substrates: very poor substrates: D-proline, glycine, no substrate: L-serine
Products: -
Products: -
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein
physiological function
involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein
physiological function
involved in biosynthesis of prodigiosin. PigI is presumed to adenylate proline and transfer the prolyl group to PigG. The prolyl-PigG is then oxidized by PigA
physiological function
an enzyme deletion mutant is white, cannot be complemented by the exogenous addition of 2-methyl-3-n-amyl-pyrrole, and produces 2-methyl-3-n-amyl-pyrrole. Enzymes PigI, PigG and PigA are involved in the early steps of 4-methoxy-2,2'-bipyrrole-5-carbaldehyde biosynthesis
physiological function
-
involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein
-
physiological function
Pseudomonas fluorescens ATCC BAA-4770
-
involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ANEB_ASPA1
Aspergillus aculeatus (strain ATCC 16872 / CBS 172.66 / WB 5094)
1078
0
119429
Swiss-Prot
other Location (Reliability: 1)
REDM_STRCO
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
532
0
56886
Swiss-Prot
-
PLTF_PSEF5
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
498
0
54537
Swiss-Prot
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to 2.0 A resolution, space group P1 with unit-cell parameters a = 51.2, b = 62.8, c = 91.3 A, alpha = 105.1°, beta = 90.1°, gamma = 92.2°
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Williamson, N.R.; Simonsen, H.T.; Ahmed, R.A.; Goldet, G.; Slater, H.; Woodley, L.; Leeper, F.J.; Salmond, G.P.
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces
Mol. Microbiol.
56
971-989
2005
Prodigiosinella confusarubida (Q5W263)
brenda
Han, N.; Ran, T.; Lou, X.; Gao, Y.; He, J.; Tang, L.; Xu, D.; Wang, W.
Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative l-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia
Acta Crystallogr. Sect. F
70
624-627
2014
Serratia sp. FS14
brenda
Thomas, M.; Burkart, M.; Walsh, C.
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis
Chem. Biol.
9
171-184
2002
Pseudomonas fluorescens (Q4KCY5), Streptomyces coelicolor (Q4KCY5), Streptomyces coelicolor A(3)2 (Q4KCY5), Pseudomonas fluorescens ATCC BAA-4770 (Q4KCY5)
brenda
Harris, A.; Williamson, N.; Slater, H.; Cox, A.; Abbasi, S.; Foulds, I.; Simonsen, H.; Leeper, F.; Salmond, G.
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation
Microbiology
150
3547-3560
2004
Prodigiosinella confusarubida (Q5W263)
brenda
EXTERNAL LINKS (specific for EC-Number 6.2.1.53)
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