We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule.
Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
The enzyme appears in viruses and cellular organisms
Synonyms
BtrJ , [BtrI acyl-carrier protein]L-glutamate ligase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
[BtrI acyl-carrier protein]L-glutamate ligase
-
-
-
BtrJ
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein] = ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
(2)
-
-
ATP + L-glutamate + BtrI acyl-carrier protein = ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
(1)
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
[BtrI acyl-carrier protein]:L-glutamate ligase (ADP-forming)
Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule.
Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + 4-aminobutanoate + BtrI acyl-carrier protein
ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein]
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein]
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
additional information
?
-
ATP + 4-aminobutanoate + BtrI acyl-carrier protein
ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein]
-
GABA-S-BtrI is closely related to holo-BtrI
?
ATP + 4-aminobutanoate + BtrI acyl-carrier protein
ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein]
-
GABA-S-BtrI is closely related to holo-BtrI
?
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
-
-
?
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
-
-
?
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein]
BtrJ is able to catalyze ligation of a second L-glutamate molecule to GABA-S-BtrI, forming an amide bond
-
?
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein]
BtrJ is able to catalyze ligation of a second L-glutamate molecule to GABA-S-BtrI, forming an amide bond
-
?
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
-
-
?
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
-
-
?
additional information
?
-
the reaction mechanism is proposed to involve the generation of an acylphosphate intermediate via hydrolysis of ATP to ADP. No activity with (2S)-4-amino-2-hydroxybutyrate, AHBA. No gamma-L-Glu-gamma-L-Glu-S-BtrI produced in the BtrJ assay
-
?
additional information
?
-
the reaction mechanism is proposed to involve the generation of an acylphosphate intermediate via hydrolysis of ATP to ADP. No activity with (2S)-4-amino-2-hydroxybutyrate, AHBA. No gamma-L-Glu-gamma-L-Glu-S-BtrI produced in the BtrJ assay
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
-
-
-
?
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
-
-
-
?
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
-
-
-
?
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mg2+
essentially required
Mn2+
essentially required, activates
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene btrJ
UniProt
brenda
gene btrJ
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
evolution
BtrJ belongs to the ATP-grasp superfamily
evolution
-
BtrJ belongs to the ATP-grasp superfamily
metabolism
butirosin biosynthetic cluster, proceeds via intermediates tethered to a specific acyl carrier protein and gamma-glutamylation of an ACP-derived gamma-aminobutyrate intermediate. The pathway involves the acyl carrier protein BtrI, an ATP-dependent ligase BtrJ, a pyridoxal phosphate-dependent decarboxylase BtrK, and a two-component flavin-dependent monooxygenase system BtrO and BtrV, overview
metabolism
-
butirosin biosynthetic cluster, proceeds via intermediates tethered to a specific acyl carrier protein and gamma-glutamylation of an ACP-derived gamma-aminobutyrate intermediate. The pathway involves the acyl carrier protein BtrI, an ATP-dependent ligase BtrJ, a pyridoxal phosphate-dependent decarboxylase BtrK, and a two-component flavin-dependent monooxygenase system BtrO and BtrV, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
BTRJ_BACCI
419
0
48648
Swiss-Prot
-
A0A5C5XGJ0_9PLAN
478
0
54179
TrEMBL
-
A0A4P6Q0B8_9ACTN
467
0
49941
TrEMBL
-
A0A178WWF7_9PSEU
456
0
49144
TrEMBL
-
A0A5C6BXK9_9BACT
472
0
52346
TrEMBL
-
A0A5C6DE73_9BACT
454
0
50876
TrEMBL
-
A0A0B0EG32_9BACT
454
0
50876
TrEMBL
-
A0A509G4X6_PAEPO
458
0
50166
TrEMBL
-
A0A1Y2NS72_STRFR
476
0
51307
TrEMBL
-
A0A178X4E8_9PSEU
405
0
42213
TrEMBL
-
A0A517R268_9BACT
470
0
52791
TrEMBL
-
A0A387HB44_9ACTN
430
0
45809
TrEMBL
-
A0A1V4SM97_RUMHU
470
0
53888
TrEMBL
-
A0A5C6BDV7_9BACT
388
0
42451
TrEMBL
-
A0A5C5X8D9_9BACT
472
0
52275
TrEMBL
-
A0A2H6GNB3_9BACT
459
0
49895
TrEMBL
-
A0A1D8FWV3_9ACTN
476
0
51335
TrEMBL
-
A0A6C0QDG5_9ACTN
465
0
49826
TrEMBL
-
A0A5B1CQM7_9BACT
475
0
52158
TrEMBL
-
A0A1V5VQF7_9BACT
445
0
50165
TrEMBL
-
A0A517ZM79_9BACT
416
0
45923
TrEMBL
-
A0A509G3P8_PAEPO
510
0
55961
TrEMBL
-
A0A1E3X480_9BACT
454
0
51311
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
48600
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
50962
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
50969
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
monomer
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
monomer
-
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
additional information
purified BtrJ shows aggregation in solution, preventable by DTT
additional information
-
purified BtrJ shows aggregation in solution, preventable by DTT
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene btrJ, butirosin genetic cluster organization, overview. Expression of N-terminal His6-tagged BtrJ
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Li, Y.; Llewellyn, N.M.; Giri, R.; Huang, F.; Spencer, J.B.
Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway
Chem. Biol.
12
665-675
2005
Bacillus circulans (Q4H4E7), Bacillus circulans NR3312 (Q4H4E7)
brenda
Select items on the left to see more content.
html completed