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Information on EC 6.1.2.2 - nebramycin 5' synthase

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EC Tree
     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.2 acid-alcohol ligases (ester synthases)
                6.1.2.2 nebramycin 5' synthase
IUBMB Comments
Requires Fe(III). The enzyme from the bacterium Streptoalloteichus tenebrarius catalyses the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin.
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The enzyme appears in viruses and cellular organisms
Synonyms
obramycin carbamoyltransferase, TacA, TobZ, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate
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(1a); (2a)
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kanamycin A + carbamoyl phosphate + ATP + H2O = 6''-O-carbamoylkanamycin A + AMP + diphosphate + phosphate
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(2) overall reaction
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O-carbamoyladenylate + kanamycin A = AMP + 6''-O-carbamoylkanamycin A
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catalytic reaction mechanism, detailed overview
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O-carbamoyladenylate + tobramycin = AMP + nebramycin 5'
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(1b)
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tobramycin + carbamoyl phosphate + ATP + H2O = nebramycin 5' + AMP + diphosphate + phosphate
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(1) overall reaction
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