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Information on EC 5.6.2.3 - DNA 5'-3' helicase

for references in articles please use BRENDA:EC5.6.2.3

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IUBMB Comments

The activity is stimulated by DNA polymerase III. As the lagging ssDNA is created, it becomes coated with S Single-Stranded DNA Binding protein (SSB). Once every 500-2000 nucleotides, primase is stimulated by DnaB helicase to synthesize a primer at the replication fork. This primer is elongated by the lagging strand half of DNA polymerase III holoenzyme.

The enzyme appears in viruses and cellular organisms
  • 5.6.2.3
  • unwind
  • single-stranded
  • atpase
  • duplex
  • ssdna
  • bacteriophage
  • dna-dependent
  • stall
  • bloom
  • telomere
  • replisome
  • primase
  • minichromosome
  • hexameric
  • werner
  • checkpoint
  • ophthalmoplegia
  • rad51
  • tfiih
  • g-quadruplexes
  • rpa
  • oxygenase-1
  • chromatid
  • datp
  • cockayne
  • interstrand
  • fancd2
  • recbcd
  • restart
  • d-loops
  • mcm4
  • loader
  • primosome
  • okazaki
  • lagging-strand
  • warsaw
  • rothmund-thomson
  • break-induced
  • pre-rcs
  • postreplication
  • dyskeratosis
  • translesion
  • helicase-primase
  • pre-replicative
  • medicine
  • blunt-ended
  • ctip
  • cdt1
  • ssdna-binding
  • recql4
  • diagnostics
  • helicase-like
Reaction Schemes
Couples ATP hydroysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.

Synonyms
dna helicase, bach1, twinkle, fancj, rtel1, dnab helicase, ddx11, mcm helicase, mcm protein, blm protein, more

REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Couples ATP hydroysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
show the reaction diagram
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