The enzyme, characterized from the bacteria Streptomyces venezuelae and Streptomyces pristinaespiralis, participates in the biosynthesis of the antibiotics chloramphenicol and pristinamycin IA, respectively. cf. EC 5.4.99.5, chorismate mutase.
The expected taxonomic range for this enzyme is: Streptomyces
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SYSTEMATIC NAME
IUBMB Comments
4-amino-4-deoxychorismate pyruvatemutase
The enzyme, characterized from the bacteria Streptomyces venezuelae and Streptomyces pristinaespiralis, participates in the biosynthesis of the antibiotics chloramphenicol and pristinamycin IA, respectively. cf. EC 5.4.99.5, chorismate mutase.
genes papA, papB, papC, papM are involved in the biosynthesis of the pristinamycin I precursor 4-dimethylamino-L-phenylalanine. PapB is a mutase responsible for the synthesis of 4-amino 4-deoxy prephenic acid
metabolic engineering of Escherichia coli for para-amino-phenylethanol and para-amino-phenylacetic acid biosynthesis. Overexpressed the heterologous genes encoding 4-amino-4-deoxychorismate synthase (pabAB from Corynebacterium glutamicum), 4-amino-4-deoxychorismate mutase and 4-amino-4-deoxyprephenate dehydrogenase (papB and papC from Streptomyces venezuelae) and ThDP-dependent keto-acid decarboxylase (aro10 from Saccharomyces cerevisiae) in Escherichia coli
Escherichia coli K-12 cells are genetically engineered for the production of p-amino-L-phenylalanine (L-PAPA) from glycerol as main carbon source. To do so, genes for a 4-amino-4-deoxychorismate synthase (pabAB from Corynebacterium glutamicum), and genes encoding a 4-amino-4-deoxychorismate mutase and a 4-amino-4-deoxyprephenate dehydrogenase (papB and papC, both from Streptomyces venezuelae) are cloned and expressed in Escherichia coli W3110 (lab strain LJ110). In shake flask cultures with minimal medium this leads to the formation of ca. 43 mg/l of L-PAPA from 5 g/l glycerol. By expression of additional chromosomal copies of the tktA and glpX genes, and of plasmid-borne aroFBL genes in a tyrR deletion strain, an improved L-PAPA producer is obtained which gave a titer of 5.47 g/l L-PAPA from 33.3 g/l glycerol in fed-batch cultivation (shake flasks). In a fed-batch fermenter cultivation, a titer of 16.7 g/l L-PAPA is obtained which is the highest so far reported value for this non-proteinogenic amino acid
The gene cluster for chloramphenicol biosynthesis in Streptomyces venezuelae ISP5230 includes novel shikimate pathway homologues and a monomodular non-ribosomal peptide synthetase gene
Identification of analysis of genes from Streptomyces pristinaespiralis encoding enzymes involved in the biosynthesis of the 4-dimethylamino-L-phenylalanine precursor of pristinamycin I
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