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Information on EC 5.3.1.7 - mannose isomerase
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5.3.1.7 mannose isomeraseIUBMB Comments
Also acts on D-lyxose and D-rhamnose.
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Word Map
- 5.3.1.7
- d-fructose
- synthesis
- d-lyxose
-
isomerized
- radiobacter
-
agrobacterium
-
isomerization
- d-xylulose
- chitosan
-
fortuitously
-
crosslinking
- albumin
- glutaraldehyde
The enzyme appears in viruses and cellular organisms
Synonyms
mannose isomerase, d-mannose isomerase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D-Mannose isomerase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-Mannose = D-fructose
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
D-mannose aldose-ketose-isomerase
Also acts on D-lyxose and D-rhamnose.
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CAS REGISTRY NUMBER
COMMENTARY
9031-25-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-fructose
D-mannose
-
the conversion of 25% D-fructose is 29.4% after 2h
-
?
D-Lyxose
D-Xylulose
reaction of EC 5.3.1.15
-
r
D-Mannose
?
-
constitutive enzyme. Since the enzymes mannokinase and phosphomannose isomerase cannot be detected, the isomerase and an inducible fructokinase must have a function in the metabolism of mannose by this bacterium
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-fructose
D-mannose
-
the conversion of 25% D-fructose is 29.4% after 2h
-
-
?
D-Mannose
?
-
constitutive enzyme. Since the enzymes mannokinase and phosphomannose isomerase cannot be detected, the isomerase and an inducible fructokinase must have a function in the metabolism of mannose by this bacterium
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Mn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
N-bromosuccinimide
-
partial inhibition at 0.1 mM, complete inhibition at 1 mM
phenylmethylsulfonyl fluoride
-
partial inhibition at 0.1 mM, complete inhibition at 1 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 10.5
-
pH 6.0: about 65% of maximal activity, pH 10.5: about 50% of maximal activity
6 - 9.5
-
pH 6: about 35% of maximal activity, pH 9.5: about 40% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45
60
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35 - 65
-
35°C: about 55% of maximal activity, 65°C: about 10% of maximal activity, 70°C: about 20% of maximal activity
40 - 68
-
40°C: about 45% of maximal activity, 68°C: about 25% of maximal activity
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mutant strain capable of growing with sucrose or fructose as carbon source
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
30% of the activity compared to mannose-grown cells
brenda
-
greatest enzyme activity
brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A0L0M9V3_9BURK
417
0
48086
TrEMBL
-
A0A3B0RQM4_9ZZZZ
402
0
46284
TrEMBL
other Location (Reliability: 5)
Q88P39_PSEPK
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
416
0
45472
TrEMBL
-
F9UQ52_LACPL
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
313
0
35210
TrEMBL
-
A0A3B0SL85_9ZZZZ
400
0
46636
TrEMBL
other Location (Reliability: 2)
A0A0L0M6B6_9BURK
399
0
46662
TrEMBL
-
A0A0J7JQJ0_9BURK
403
0
47061
TrEMBL
-
G4M4N0_9BURK
417
0
48309
TrEMBL
-
A0A0L0M3X0_9BURK
408
0
47552
TrEMBL
-
F9UMZ8_LACPL
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
315
0
35272
TrEMBL
-
D9RZ53_THEOJ
Thermosediminibacter oceani (strain ATCC BAA-1034 / DSM 16646 / JW/IW-1228P)
181
0
20841
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
44000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40
-
immobilized enzyme loses 46% of its activity after 4 d, soluble enzyme loses 45% of its activity after 4 d
60
70
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no loss of activity after 24 h dialysis against 50 mM Tris-HCl buffer containing 1 mM EDTA
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
-
potential use of immobilized enzyme in a process for the production of fructose syrups containing a higher proportion of fructose
synthesis
AJH12524
expression in Bacillus subtilis leads to secretion of enzyme. Under optimum conditions, production of D-mannose using crude enzyme reaches about 150 g/l with approximately 25% turnover yield
synthesis
-
optimization of fermentation medium for producing intracellular D-mannose isomerase. The optimized medium containing 1.3% w/v D-mannose, and 0.055 % soybean meal results in 1.32fold enhanced D-mannose production of 1.638 Units/ml
synthesis
under optimum conditions, 101.6 g/l D-mannose is produced from 400 g/l D-fructose after reaction for 9 h, giving a conversion yield of 25.4%
synthesis
-
under optimum conditions, 101.6 g/l D-mannose is produced from 400 g/l D-fructose after reaction for 9 h, giving a conversion yield of 25.4%
synthesis
Thermosediminibacter oceani DSM 1664
-
under optimum conditions, 101.6 g/l D-mannose is produced from 400 g/l D-fructose after reaction for 9 h, giving a conversion yield of 25.4%
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Noltmann, E.A.
Aldose-ketose isomerases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
6
271-354
1972
Mycolicibacterium smegmatis, Lentzea aerocolonigenes, Pelomonas saccharophila, Acidovorax avenae, Acidovorax avenae S-48
-
brenda
Palleroni, N.J.; Doudoroff, M.
Mannose isomerase of Pseudomonas saccharophila
J. Biol. Chem.
218
535-548
1955
Pelomonas saccharophila
brenda
Hey-Ferguson, A.; Elbein, A.D.
Purification of a D-mannose isomerase from Mycobacterium smegmatis
J. Bacteriol.
101
777-780
1970
Mycolicibacterium smegmatis
brenda
Stevens, F.J.; Wu, T.T.
Growth on D-lyxose of a mutant strain of Escherichia coli K12 using a novel isomerase and enzymes related to D-xylose metabolism
J. Gen. Microbiol.
97
257-265
1976
Escherichia coli, Acidovorax avenae, Acidovorax avenae S-48
brenda
Stevens, F.J.; Stevens, P.W.; Hovis, J.G.; Wu, T.T.
Some properties of D-mannose isomerase from Escherichia coli K12
J. Gen. Microbiol.
124
219-223
1981
Escherichia coli
brenda
Allenza, P.; Morrell, M.J.; Detroy, R.W.
Conversion of mannose to fructose by immobilized mannose isomerase from Pseudomonas cepacia
Appl. Biochem. Biotechnol.
24-25
171-182
1990
Burkholderia cepacia
brenda
Sauerstein, J.; Ramm, M.; Schubert, K.; Reuter, G.
Nachweis einer direkten Isomerisierung von Mannose zu Fructose und ihre Bedeutung fur den Mannosestoffwechsel von Pseudomonas syringae pv. phaseolicola
J. Basic Microbiol.
28
673-679
1988
Pseudomonas savastanoi pv. phaseolicola
-
brenda
Takasaki, Y.; Hinoki, K.; Kataoka, Y.; Fukuyama, S.; Nishimura, N.; Hayashi, S.; Imada, K.
Enzymatic production of D-mannose from D-fructose by mannose isomerase
J. Ferment. Bioeng.
76
237-239
1993
Pseudomonas sp.
-
brenda
Hirose, J.; Maeda, K.; Yokoi, H.; Takasaki, Y.
Purification and characterization of mannose isomerase from Agrobacterium radiobacter M-1
Biosci. Biotechnol. Biochem.
65
658-661
2001
Agrobacterium tumefaciens, Agrobacterium tumefaciens M-1
brenda
Liu, Q.; Li, Q.; Yuan, Q.; Feng, W.
Expression of D-mannose isomerase in E. coli and a study of its catalytic activity
J. Beijing Univ. Chem. Technol.
38
101-104
2011
Agrobacterium tumefaciens
-
brenda
Siangsuepchart, A.; Lumyong, S.
Statistical optimization for the production of D-mannose isomerase by Saccharothrix sp. CMU-K747 using response surface methodology
Chiang Mai J. Sci.
42
52-62
2015
Saccharothrix sp. CMU-K747
-
brenda
Hu, X.; Zhang, P.; Miao, M.; Zhang, T.; Jiang, B.
Development of a recombinant D-mannose isomerase and its characterizations for D-mannose synthesis
Int. J. Biol. Macromol.
89
328-335
2016
Escherichia coli (AJH12524)
brenda
Yu, L.; Zhang, W.; Zhang, T.; Jiang, B.; Mu, W.
Efficient biotransformation of D-fructose to D-mannose by a thermostable D-lyxose isomerase from Thermosediminibacter oceani
Process Biochem.
51
2026-2033
2016
Thermosediminibacter oceani (D9RZ53), Thermosediminibacter oceani DSM 16646 (D9RZ53)
-
brenda
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