the purfied recombinant His-tagged AcbO protein is actively converting the product of the AcbM-catalysed reaction in absence of any other cofactor or coenzyme
the enzyme is involved in the acarbose biosynthesis, pathway overview. The biosynthesis of the C7-cyclitol, called valienol (or valienamine), of the alpha-glucosidase inhibitor acarbose starts from the cyclization of sedo-heptulose 7-phosphate to 2-epi-5-epivaliolone. Synthesis of the intermediate 2-epi-5-epi-valiolone is catalyzed by the cyclase AcbC encoded in the biosynthetic (acb) gene cluster of Actinoplanes sp. SE50/110. The cyclitol precursor 2-epi-5-epi-valiolone is phosphorylated, forming the intermediate 2-epi-5-epi-valiolone 7-phosphate, by the enzyme AcbM as a first step in its conversion to the valienol moiety. Epimerase AcbO catalyzes the next conversion step, leading to an isomeric phosphorylated substance with the same molecular mass, most likely the epimerization product of 2-epi-5-epivaliolone 7-phosphate to 5-epi-valiolone 7-phosphate
the enzyme is involved in the acarbose biosynthesis, pathway overview. The C7-cyclitol 2-epi-5-epi-valiolone is the first precursor of the cyclitol moiety of the alpha-glucosidase inhibitor acarbose in Actinoplanes sp. SE50. The 2-epi-5-epi-valiolone becomes phosphorylated at C7 by the ATP-dependent kinase AcbM prior to the next modifications
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UNIPROT
LITERATURE
gene acbO, encoded in the acbKMLNOC operon, DNa and amino acid sequence determination and analysis, overexpression of N-terminally His-tagged enzyme in Streptomyces lividans strain TK64, expression in Escherichia coli is not successful, method development, overview
gene acbO, encoded in the acbKMLNOC operon, overexpression of N-terminally His-tagged enzyme in Streptomyces lividans strain TK64 under the control of an inducible promoter (ptipA)
Zhang, C.S.; Stratmann, A.; Block, O.; Brückner, R.; Podeschwa, M.; Altenbach, H.J.; Wehmeier, U.F.; Piepersberg, W.
Biosynthesis of the C(7)-cyclitol moiety of acarbose in Actinoplanes species SE50/110. 7-O-Phosphorylation of the initial cyclitol precursor leads to proposal of a new biosynthetic pathway
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5.1.3.35 2-epi-5-epi-valiolone 7-phosphate 2-epimerase
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