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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
L-ornithine racemase,
or-5 , Racemase, ornithine,
STM2358 ,
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Racemase, ornithine
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L-ornithine = D-ornithine
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D-alanine
L-alanine
conversion is lower than 10% compared to conversion of L-ornithine
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r
L-arginine
D-arginine
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r
L-asparagine
D-asparagine
conversion is lower than 10% compared to conversion of L-ornithine
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r
L-lysine
D-lysine
very low activity
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r
L-serine
D-serine
conversion is 10% compared to conversion of L-ornithine
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r
additional information
?
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L-Ornithine
D-Ornithine
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r
L-Ornithine
D-Ornithine
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isomeric form not specified
the product of this reaction is not identical with D-ornithine, the literature reference is probably incorrect
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L-Ornithine
D-Ornithine
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enzyme is strictly specific
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?
L-Ornithine
D-Ornithine
preferred substrate
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r
additional information
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the recombinant gene STM2358 product has ornithine racemase activity, no measureable alanine racemase activity of the enzyme
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additional information
?
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the recombinant gene STM2358 product has ornithine racemase activity, no measureable alanine racemase activity of the enzyme
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L-Ornithine
D-Ornithine
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r
L-Ornithine
D-Ornithine
preferred substrate
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r
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pyridoxal 5'-phosphate
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pyridoxal 5'-phosphate
dependent on
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0.77
L-ornithine
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pH 8.5, room temperature
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980
L-ornithine
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pH 8.5, room temperature
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UniProt
brenda
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brenda
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TREMBL
brenda
the product of this reaction is not identical with D-ornithine, the literature reference is probably incorrect
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brenda
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metabolism
catalyzes the first step in the ornithine fermentation pathway
evolution
a BLAST search using the translated amino acid sequence of STM2358 results in significant higher similarity (37% identity) with sequences of ornithine racemase than with alanine racemase (27% identity), suggesting that it may be an ornithine racemase
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21500
2 * 21500, gel filtration
46800
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2 * 46800, SDS-PAGE
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dimer
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2 * 46800, SDS-PAGE
homodimer
2 * 21500, gel filtration
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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expressed His-tagged recombinant protein
gene STM2358, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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Somack, R.; Costilow, R.N.
Purification and properties of a pyridoxal phosphate and coenzyme B12 dependent D-alpha-ornithine 5,4-aminomutase
Biochemistry
12
2597-2604
1973
Acetoanaerobium sticklandii
brenda
Chen, H.P.; Lin, C.F.; Lee, Y.J.; Tsay, S.S.; Wu, S.H.
Purification and properties of ornithine racemase from Clostridium sticklandii
J. Bacteriol.
182
2052-2054
2000
Acetoanaerobium sticklandii
brenda
Fonknechten, N.; Perret, A.; Perchat, N.; Tricot, S.; Lechaplais, C.; Vallenet, D.; Vergne, C.; Zaparucha, A.; Le Paslier, D.; Weissenbach, J.; Salanoubat, M.
A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine
J. Bacteriol.
191
3162-3167
2009
Acetoanaerobium sticklandii (C1FW08)
brenda
Phillips, R.S.; Poteh, P.; Miller, K.A.; Hoover, T.R.
STM2360 encodes a D-ornithine/D-lysine decarboxylase in Salmonella enterica serovar typhimurium
Arch. Biochem. Biophys.
634
83-87
2017
Salmonella enterica subsp. enterica serovar Typhimurium (Q8ZNC6), Salmonella enterica subsp. enterica serovar Typhimurium
brenda
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History
Enzyme Nomenclature
5.1.1.12 ornithine racemase
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