Any feedback?
Information on EC 4.6.1.1 - adenylate cyclase and Organism(s) Pseudomonas aeruginosa
for references in articles please use BRENDA:EC4.6.1.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 4 Lyases
4.6 Phosphorus-oxygen lyases
4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
4.6.1.1 adenylate cyclase
IUBMB Comments
Also acts on dATP to form 3′,5′-cyclic dAMP. Requires pyruvate. Activated by NAD+ in the presence of EC 2.4.2.31 NAD(P)+—arginine ADP-ribosyltransferase.
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
- 4.6.1.1
- camp
- agonist
- forskolin
-
beta-adrenergic
- pertussis
- phosphodiesterase
- isoproterenol
- prostaglandin
- gtp
- dopamine
- monophosphate
- cholera
- subtypes
- guanine
- pka
-
desensitization
- phospholipase
-
forskolin-stimulated
- g-proteins
-
camp-dependent
- epinephrine
-
pacap
- catecholamine
- parathyroid
- norepinephrine
-
adrenergic
-
muscarinic
-
vasoactive
-
guanylate
-
receptor-mediated
-
beta-adrenoceptor
-
protein-coupled
- glucagon
- bordetella
- vasopressin
-
toxin-sensitive
- naf
-
inotropic
-
gtp-binding
- 3',5'-monophosphate
- carbachol
- propranolol
-
radioligands
-
dibutyryl
- ibmx
-
adp-ribosylation
- somatostatin
- isoprenaline
- medicine
- pharmacology
- drug development
- rolipram
- 3-isobutyl-1-methylxanthine
- analysis
- molecular biology
showSynonyms
adenylate cyclase, adenylyl cyclase, adenyl cyclase, pituitary adenylate cyclase, edema factor, adenylylcyclase, soluble adenylyl cyclase, adenylate cyclase toxin, adcy5, aciii, more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3',5'-cyclic AMP synthetase
-
-
-
-
AC2
-
-
-
-
AC3
-
-
-
-
ACTP10
-
-
-
-
adenyl cyclase
-
-
-
-
Adenylate cyclase, olfactive type
-
-
-
-
adenylyl cyclase
Adenylyl cyclase type 10
-
-
-
-
adenylylcyclase
-
-
-
-
ATP pyrophosphate-lyase
-
-
-
-
Ca(2+)-inhibitable adenylyl cyclase
-
-
-
-
Ca(2+)/calmodulin activated adenylyl cyclase
-
-
-
-
CyaB
cyclase, adenylate
-
-
-
-
Edema factor
-
-
-
-
Rutabaga protein
-
-
-
-
xlAC
-
-
-
-
adenylyl cyclase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
P-O bond cleavage
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP diphosphate-lyase (cyclizing; 3',5'-cyclic-AMP-forming)
Also acts on dATP to form 3',5'-cyclic dAMP. Requires pyruvate. Activated by NAD+ in the presence of EC 2.4.2.31 NAD(P)+---arginine ADP-ribosyltransferase.
CAS REGISTRY NUMBER
COMMENTARY
9012-42-4
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP
3',5'-cAMP + diphosphate
-
Substrates: cAMP is required for expression of the secretion system by which virulence factors are translocated to the host cell
Products: -
Products: -
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
-
required, the enzyme has higher specific activity for Mn2+-ATP versus Mg2+-ATP
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
crude extract of cytosolic proteins from CHO cells
-
at least 500fold increased adenylate cyclase activity
-
additional information
-
is activated by different host cell factors and is inactive until injected into the host cell
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
the N-terminal half of the class IIIb AC consists of a predicted membrane-associated sensor domain
Highest Expressing Human Cell Lines
Cell Line LinksGENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
the Chp chemotaxis-like system regulates intracellular cAMP levels by modulating CyaB activity
physiological function
the enzyme is a key regulator of Pseudomonas aeruginosa virulence
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 12% (w/v) polyethylene glycol 20000
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A197T
the mutant shows increased activity compared to the wild type enzyme
E189R
the mutant shows increased activity compared to the wild type enzyme
E377G
the mutant shows increased activity compared to the wild type enzyme
F399H
the mutant shows increased activity compared to the wild type enzyme
F399I
the mutant shows increased activity compared to the wild type enzyme
I352T
the mutant shows increased activity compared to the wild type enzyme
L326P
the mutant shows increased activity compared to the wild type enzyme
r23ExoY
-
mutant with histidine tag at carboxyl-terminal position, detectable enzyme activity
R318W
the mutant shows increased activity compared to the wild type enzyme
R412H
the mutant shows slightly increased activity compared to the wild type enzyme
R456L
the mutant shows strongly increased activity compared to the wild type enzyme
rExoY
-
mutant with histidine tag at amino-terminal position, detectable enzyme activity
additional information
-
disruption of a class IIIb AC gene results in strong attenuation
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography and Superose 12 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli SG13009[pREP4] andM15[pREP4] cells
the putative catalytic domain (amino acids 217-463) as an N-terminal hexahistidine fusion is expressed in Escherichia coli M15[pREP4] cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yahr, T.L.; Vallis, A.J.; Hancock, M.K.; Barbieri, J.T.; Frank, D.W.
ExoY, an adenylate cyclase secreted by the Pseudomonas aeruginosa type III system
Proc. Natl. Acad. Sci. USA
95
13899-13904
1998
Pseudomonas aeruginosa
Linder, J.U.
Class III adenylyl cyclases: molecular mechanisms of catalysis and regulation
Cell. Mol. Life Sci.
63
1736-1751
2006
Bacillus anthracis, Bordetella pertussis, Saccharomyces cerevisiae, Caenorhabditis elegans, Chlamydomonas reinhardtii, Chloroflexus aurantiacus, Dictyostelium discoideum, Drosophila melanogaster, Escherichia coli, Euglena gracilis, Mus musculus, Myxococcus xanthus, Pseudomonas aeruginosa, Rattus norvegicus, Sinorhizobium meliloti, Schizosaccharomyces pombe, Arthrospira platensis, Trypanosoma brucei, Ustilago maydis, Yersinia enterocolitica, Mycobacterium tuberculosis (P9WQ35), Nostoc sp. PCC 7120 = FACHB-418 (Q7A2D9), Nostoc sp. PCC 7120 = FACHB-418 (Q8YMH0), Nostoc sp. PCC 7120 = FACHB-418 (Q8YVS0), Mycobacterium tuberculosis H37Rv (P9WQ35)
Sinha, S.C.; Sprang, S.R.
Structure, mechanism, regulation and evolution of class III nucleotidyl cyclases
Rev. Physiol. Biochem. Pharmacol.
157
105-140
2007
Aeromonas hydrophila, Bacillus anthracis, Prevotella ruminicola, Bordetella pertussis, Canis lupus familiaris, Caulobacter vibrioides, Escherichia sp., Haemophilus sp., Mycobacterium tuberculosis, Yersinia pestis, Pseudomonas sp., Pseudomonas aeruginosa, Rattus norvegicus, Rhizobium etli, Sinorhizobium meliloti, Arthrospira platensis, Trypanosoma brucei, Vibrio parahaemolyticus, Yersinia sp.
Fulcher, N.B.; Holliday, P.M.; Klem, E.; Cann, M.J.; Wolfgang, M.C.
The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity
Mol. Microbiol.
76
889-904
2010
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAK
Topal, H.; Fulcher, N.B.; Bitterman, J.; Salazar, E.; Buck, J.; Levin, L.R.; Cann, M.J.; Wolfgang, M.C.; Steegborn, C.
Crystal structure and regulation mechanisms of the CyaB adenylyl cyclase from the human pathogen Pseudomonas aeruginosa
J. Mol. Biol.
416
271-286
2012
Pseudomonas aeruginosa (Q9HZ23), Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692 (Q9HZ23)
EXTERNAL LINKS (specific for EC-Number 4.6.1.1)
Transporter Classification Database (TCDB): 8.A.85.1.4
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
