Information on EC 4.6.1.1 - adenylate cyclase and Organism(s) Homo sapiens

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Homo sapiens


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria


The taxonomic range for the selected organisms is: Homo sapiens

EC NUMBER
COMMENTARY hide
4.6.1.1
-
RECOMMENDED NAME
GeneOntology No.
adenylate cyclase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
P-O bond cleavage
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
purine metabolism
-
-
Purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP diphosphate-lyase (cyclizing; 3',5'-cyclic-AMP-forming)
Also acts on dATP to form 3',5'-cyclic dAMP. Requires pyruvate. Activated by NAD+ in the presence of EC 2.4.2.31 NAD(P)+---arginine ADP-ribosyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-42-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP
3',5'-cAMP + diphosphate
show the reaction diagram
ATP
3',5'-cyclic AMP + diphosphate
show the reaction diagram
ATP
3',5'-cyclic-AMP + diphosphate
show the reaction diagram
ATP
cAMP + diphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP
3',5'-cyclic AMP + diphosphate
show the reaction diagram
ATP
3',5'-cyclic-AMP + diphosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
bicarbonate
activates
NaF
-
activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1alpha,9alpha-dihydroxy-labd-13(E)-ene-8a,15-diol
-
a derivative of each of the two stereoisomers of labd-13(E)-ene-8a,15-diol, overview
1alpha-hydroxy-labd-13(E)-ene-8a,15-diol
-
a derivative of each of the two stereoisomers of labd-13(E)-ene-8a,15-diol, overview
2',3'-O-(2,4,6-trinitrophenyl)-ADP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-AMP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-ATP
-
most potent inhibitor for isoform ACV
2',3'-O-(2,4,6-trinitrophenyl)-CTP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-GDP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-GTP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-UTP
-
most potent inhibitor for isoforms ACI and ACII
2',3'-O-(N-anthraniloyl)-ADP
-
-
2',3'-O-(N-anthraniloyl)-ATP
-
-
2',3'-O-(N-anthraniloyl)-IMP
-
-
2',3'-O-(N-methylanthraniloyl)-ADP
-
-
2',3'-O-(N-methylanthraniloyl)-ATP
-
-
2',3'-O-(N-methylanthraniloyl)-CDP
-
-
2',3'-O-(N-methylanthraniloyl)-CTP
-
-
2',3'-O-(N-methylanthraniloyl)-GTP
-
-
2',3'-O-(N-methylanthraniloyl)-GTPgammaS
-
potent inhibitor of isoform AC5
2',3'-O-(N-methylanthraniloyl)-IDP
-
-
2',3'-O-(N-methylanthraniloyl)-IMP
-
-
2',3'-O-(N-methylanthraniloyl)-ITP
-
-
2',3'-O-(N-methylanthraniloyl)-ITPgammaS
-
potent inhibitor of isoform AC5
2',3'-O-(N-methylanthraniloyl)-UDP
-
-
2',3'-O-(N-methylanthraniloyl)-UTP
-
-
2',3'-O-(N-methylanthraniloyl)-XTP
-
-
2',5'-dideoxyadenosine
massive inhibition of cAMP formation with a combination of enzyme inhibitors 9-(tetrahydro-29-furyl)adenine and 2',5'-dideoxyadenosine; massive inhibition of cAMP formation with a combination of enzyme inhibitors 9-(tetrahydro-29-furyl)adenine and 2',5'-dideoxyadenosine
2'-deoxy-3'-O-[2-(methylamino)benzoyl]adenosine 5'-triphosphate
-
-
2'-deoxy-3'-O-[2-(methylamino)benzoyl]guanosine 5'-triphosphate
-
-
2-hydroxy-17beta-estradiol
-
specific sAC inhibitor, blocks CO2/HCO3- mediated cAMP production
2-hydroxyestradiol
-
reversible inhibition of recombinant soluble enzyme in dose dependent manner, IC50 in low micromolar range, trans-membrane enzyme VII IC50 of about 2 microM
2-hydroxyestrone
-
reversible inhibition of recombinant soluble enzyme in dose dependent manner, IC50 in low micromolar range
2OH-17beta estradiol
-
inhibits bicarbonate-induced cAMP production
3'-deoxy-2'-O-[2-(methylamino)benzoyl]adenosine 5'-triphosphate
-
-
3'-deoxy-2'-O-[2-(methylamino)benzoyl]guanosine 5'-triphosphate
-
-
4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid
-
-
4-hydroxyestradiol
-
reversible inhibition of recombinant soluble enzyme in dose dependent manner, IC50 in low micromolar range
arginine-vasopressin
-
-
ATP
-
substrate inhibition at high concentrations, inhibition is relieved in the presence of HCO3-, no substrate inhibition in the presence of 50 mM HCO3-
buprenorphine
-
a low-efficacy partial mu-opioid agonist
Ca2+/calcineurin
-
-
-
Carbachol
-
increases intracellular calcium, inhibits A2bR mediated increase in cAMP by 53%
DAMGO
-
a higher efficacy mu-opioid agonist
diphosphate
-
non-competitive inhibitor with respect to ATP
EDTA
-
-
Galphai
-
inhibits Galphas-stimulated activities of ACVI, Gbetagamma does not alter the ability of Galphai to inhibit the activities of ACVI
-
GALPHAI protein
-
inhibits basal activity of isoform V, not the basal activity of isoform VI
-
inhibitory G protein
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L-(+)-2,3-butanediol
-
isoform AC7 activity is inhibited to about 50% with 100 mM L-(+)-2,3-butanediol
labd-13(E)-ene-8a,15-diol
-
isolated from the resin Ladano of the plant Cistus creticus subsp. creticus that grows in the island of Crete, Greece. Docking calculations of the two stereoisomers of the compound and derivatives to the enzyme at the forskolin binding site, overview
lubrol-PX
-
-
morphine
-
a higher efficacy mu-opioid agonist
nalbuphine
-
a low-efficacy partial mu-opioid agonist
NO
-
NO functions either via an as yet unidentified regulator of adenylyl cyclase or the enzyme itself is the target of NO, inhibition is reversed by reducing agents
Oxytocin
-
-
PAPANANOATE
-
nitric oxide donor, completely abolishes A2bR mediated cAMP production
phosphocreatine
-
-
prostaglandin E1
-
-
putrescine
-
-
quinpirole
-
-
S-nitroso-N-acetylpenicillamine
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nitric oxide donor, inhibits A2bR mediated increase in cAMP by 64%
spermidine
-
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spermine
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SQ 22536
i.e. 9-(tetrahydro-29-furyl)adenine, massive inhibition of cAMP formation with a combination of enzyme inhibitors 9-(tetrahydro-29-furyl)adenine and 2',5'-dideoxyadenosine; i.e. 9-(tetrahydro-29-furyl)adenine, massive inhibition of cAMP formation with a combination of enzyme inhibitors 9-(tetrahydro-29-furyl)adenine and 2',5'-dideoxyadenosine
SQ22536
thrombin
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thrombin transiently inhibits adenylyl cyclase 6
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tyrphostin A25
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IC50 0.119mM, IC50 forskolin-stimulated adenylyl cyclase 1 mM, 37C, pH 7.5
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2,3-propanetriol
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isoform AC6 shows about 10% stimulation of activity with 100 mM 1,2,3-propanetriol, isoform AC7 shows about 18% stimulation of activity with 100 mM 1,2,3-propanetriol, isoform AC9 shows about 15% stimulation of activity with 100 mM 1,2,3-propanetriol
1,2-Butanediol
-
isoform AC6 shows about 37% stimulation of activity with 100 mM 1,2-butanediol, isoform AC7 shows about 38% stimulation of activity with 100 mM 1,2-butanediol, isoform AC9 shows about 32% stimulation of activity with 100 mM 1,2-butanediol
1,3-butanediol
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isoforms AC6 and AC7 show about 22% stimulation of activity with 100 mM 1,3-butanediol, isoform AC9 shows about 17% stimulation of activity with 100 mM 1,3-butanediol
1,3-Propanediol
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isoform AC6 shows about 17% stimulation of activity with 100 mM 1,3-propanediol, isoform AC7 shows about 20% stimulation of activity with 100 mM 1,3-propanediol, isoform AC9 shows about 17% stimulation of activity with 100 mM 1,3-propanediol
1,4-Butanediol
-
isoform AC6 shows about 30% stimulation of activity with 100 mM 1,4-butanediol, isoform AC7 shows about 25% stimulation of activity with 100 mM 1,4-butanediol, isoform AC9 shows about 40% stimulation of activity with 100 mM 1,4-butanediol
1-butanol
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isoform AC6 shows about 85% stimulation of activity with 100 mM 1-butanol, isoform AC7 shows about 110% stimulation of activity with 100 mM 1-butanol, isoform AC9 shows about 105% stimulation of activity with 100 mM 1-butanol
1-propanol
-
isoform AC6 shows about 31% stimulation of activity with 100 mM 1-propanol, isoform AC7 shows about 50% stimulation of activity with 100 mM 1-propanol, isoform AC9 shows about 44% stimulation of activity with 100 mM 1-propanol
2,3-Butanediol
-
isoform AC6 shows about 30% stimulation of activity with 100 mM 2,3-butanediol, isoform AC7 shows about 35% stimulation of activity with 100 mM 2,3-butanediol, isoform AC9 shows about 41% stimulation of activity with 100 mM 2,3-butanediol
2-butanol
-
isoform AC6 shows about 60% stimulation of activity with 100 mM 2-butanol, isoform AC7 shows about 70% stimulation of activity with 100 mM 2-butanol, isoform AC9 shows about 60% stimulation of activity with 100 mM 2-butanol
2-propanol
-
isoform AC6 shows about 20% stimulation of activity with 100 mM 2-propanol, isoform AC7 shows about 35% stimulation of activity with 100 mM 2-propanol, isoform AC9 shows about 40% stimulation of activity with 100 mM 2-propanol
A2B adenosine receptor
-
activates the enzyme and regulates its activity and the cAMP signaling pathway in mast and microvascular cell, role of the C-terminus of the A2B receptor in stimulation of adenylate cyclase, which is important for A2B receptor coupling to Gs-adenylate cyclase, overview
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A2bR
-
-
-
Calmodulin
Carbachol
-
carbachol-induced capacitative Ca2+ entry clearly stimulates AC8-mediated cAMP production at the single-cell level
catecholamine
-
activation
CO2
-
induces acidification of cells, accompanied by a rise in intracellular HCO3-
D-(-)-2,3-butanediol
-
isoforms AC6 and AC7show about 30% stimulation of activity with 100 mM D-(-)-2,3-butanediol, isoform AC9 shows about 10% stimulation of activity with 100 mM D-(-)-2,3-butanediol
D1A dopamine receptor
-
-
-
ethanol
forskolin
G-protein
activation by betagamma subunits; betagamma subunit; betagamma subunits
-
G-protein alpha-subunit
-
-
-
Galphas
-
GALPHAS protein
-
250fold stimulation isoform V, 500fold stimulation isoform VI
-
Gbeta1gamma2
-
increases the activation of ACV and ACVI by forskolin or Galphas. Gbetagamma subunits derived from sources other than Gi participate in mediating the full activation of ACVI by the beta-adrenergic receptor agonist, isoproterenol
-
Gsalpha
-
AC5 undergoes a cooperative activation by Gsalpha
-
GTPgammaS
guanosine 5'-(beta,gamma-imido)triphosphate
-
activation
HCO3-
isoprenaline
-
-
isoproterenol
L-(+)-2,3-butanediol
-
isoform AC6 shows about 55% stimulation of activity with 100 mM L-(+)-2,3-butanediol, isoform AC9 shows about 45% stimulation of activity with 100 mM L-(+)-2,3-butanediol
meso-2,3-butanediol
-
isoform AC6 shows about 15% stimulation of activity with 100 mM meso-2,3-butanediol, isoform AC7 shows about 28% stimulation of activity with 100 mM meso-2,3-butanediol, isoform AC9 shows about 10% stimulation of activity with 100 mM meso-2,3-butanediol
NaF
-
activation
NaHCO3
-
10 mM, 30fold activation, half-maximal activation at 11 mM
PKC
-
-
-
prostaglandin E1
-
-
protein kinase A
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stimulates cAMP in the brain, the activation is increased in brains of patients with bipolar mood disorders, overview
-
Protein kinase C
phosphorylation
-
relaxin
-
AC5 activity is potentiated by PKCzeta after exposure to relaxin
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Sodium fluoride
-
-
sphingosine 1-phosphate
stimulates
stimulating G protein
-
thapsigargin
-
thapsigargin-induced capacitative Ca2+ entry clearly stimulates AC8-mediated cAMP production at the single-cell level
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011 - 0.8
ATP
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00014 - 0.0011
2',3'-O-(2,4,6-trinitrophenyl)-ADP
0.0048 - 0.0074
2',3'-O-(2,4,6-trinitrophenyl)-AMP
0.0000037 - 0.000099
2',3'-O-(2,4,6-trinitrophenyl)-ATP
0.000024 - 0.00011
2',3'-O-(2,4,6-trinitrophenyl)-CTP
0.00041 - 0.0034
2',3'-O-(2,4,6-trinitrophenyl)-GDP
0.000023 - 0.00022
2',3'-O-(2,4,6-trinitrophenyl)-GTP
0.0000071 - 0.000024
2',3'-O-(2,4,6-trinitrophenyl)-UTP
0.00064 - 0.0029
2',3'-O-(N-anthraniloyl)-ADP
0.00012 - 0.00064
2',3'-O-(N-anthraniloyl)-ATP
0.0043 - 0.0075
2',3'-O-(N-anthraniloyl)-IMP
0.00079 - 0.0029
2',3'-O-(N-methylanthraniloyl)-ADP
0.0001 - 0.00033
2',3'-O-(N-methylanthraniloyl)-ATP
0.00058 - 0.0037
2',3'-O-(N-methylanthraniloyl)-CDP
0.00015 - 0.00069
2',3'-O-(N-methylanthraniloyl)-CTP
0.000053 - 0.00061
2',3'-O-(N-methylanthraniloyl)-GTP
0.000034 - 0.00037
2',3'-O-(N-methylanthraniloyl)-GTPgammaS
0.000031 - 0.000086
2',3'-O-(N-methylanthraniloyl)-IDP
0.0044 - 0.0085
2',3'-O-(N-methylanthraniloyl)-IMP
0.0000012 - 0.000014
2',3'-O-(N-methylanthraniloyl)-ITP
0.000032 - 0.00012
2',3'-O-(N-methylanthraniloyl)-ITPgammaS
0.00034 - 0.0027
2',3'-O-(N-methylanthraniloyl)-UDP
0.000032 - 0.00046
2',3'-O-(N-methylanthraniloyl)-UTP
0.0011 - 0.003
2',3'-O-(N-methylanthraniloyl)-XTP
0.00032 - 0.0048
2'-deoxy-3'-O-[2-(methylamino)benzoyl]adenosine 5'-triphosphate
0.00027 - 0.0013
2'-deoxy-3'-O-[2-(methylamino)benzoyl]guanosine 5'-triphosphate
0.000065 - 0.00054
3'-deoxy-2'-O-[2-(methylamino)benzoyl]adenosine 5'-triphosphate
0.0018 - 0.0087
3'-deoxy-2'-O-[2-(methylamino)benzoyl]guanosine 5'-triphosphate
1
GTP
-
in 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 10 mM MgCl2, 10 mM CaCl2, at 37C
additional information
additional information
-
inhibition of forskolin-stimulated adenylyl cyclase by mu-opioid recptor agonists in cells expressing different forms of G proteins, overview
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
2-hydroxyestradiol
Homo sapiens;
-
reversible inhibition of recombinant soluble enzyme in dose dependent manner, IC50 in low micromolar range, trans-membrane enzyme VII IC50 of about 2 microM
0.043
4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid
Homo sapiens;
-
in 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 10 mM MgCl2, 10 mM CaCl2, at 37C
0.000022
arginine-vasopressin
Homo sapiens;
-
-
0.0000034
Oxytocin
Homo sapiens;
-
-
0.119
tyrphostin A25
Homo sapiens;
-
IC50 0.119mM, IC50 forskolin-stimulated adenylyl cyclase 1 mM, 37C, pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
Vmax 0.010 nmol/min/mg, wild type isoform VI, 30C; Vmax 0.039 nmol/min/mg, mutant isoform V N1090D, 30C; Vmax 0.077 nmol/min/mg, wild type isoform V, 30C; Vmax 0.089 nmol/min/mg, mutant isoform VI F1078S, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
ciliary beat frequency is stimulated by cAMP independently of intracellular pH
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
forskolin-insensitive AC isoforms in various B cell populations
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
erythroleukemia cell line, high expression of isozyme AC7
Manually annotated by BRENDA team
-
cardiac
Manually annotated by BRENDA team
a macrophage-like cell line
Manually annotated by BRENDA team
-
specific localization of the 50 kDa isoform to the axoneme of ciliated airway epithelial cells
Manually annotated by BRENDA team
-
expresses all nine adenylate cyclase isoforms
Manually annotated by BRENDA team
additional information
-
AC1 and AC8 present at the apical pool of airway epithelial cells, specifically in ciliated cells, AC3 mainly present on the basolateral aspect
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
in SH-SY5Y cells AC9 mainly in irregular discrete punctae which are distinct from lysosomes, early endosomes or tubulovesicular endosomes
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
UNIPROT
ORGANISM
Homo sapiens;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
-
sAC form in axonemes, Western blot analysis
75000
-
Western blot analysis
190000
192000
-
AC9, Western blot analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
glycosylation at N955 and N964
phosphoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallized in complex with substrate and Ca2+ or Mg2+
-
hanging drop vapor diffusion method, using 100 mM sodium acetate (pH 4.8), 200 mM trisodium-citrate, 18% (w/v) PEG 4000, 20% (v/v) glycerol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ACV purified by gel filtration
-
by gel filtration
-
glutathione-Sepharose 4B resin column chromatography, Q-Sepharose column chromatography, and Talon resin column chromatography
-
nickel affinity column chromatography and gel filtration
-
recombinant sAC
-
soluble isozyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ACV fused with a GST tag and expressed in Escherichia coli, wild-type and mutant ACVIdeltaN enzymes expressed in Sf9 cells, COS-7 cells overexpressing ACVI, binding domain vector pGBKT7 together with pGADT7-beta1 or the activating vector pGADT7 together with pGBKT7-N-terminal region of ACV or ACVI co-transformed into AH109 cells
-
chimeras inserted into the mammalian expression vector pCMV-SK, wild-type and chimeric mutants expressed in HEK-293 cells
cloning of AC7 cDNA from HEL cells; gene Adcy7 encoding isozyme AC7
expressed in C6 glioma cells
-
expressed in Escherichia coli in Hi5 cells
-
expressed in Sf9 insect cells
-
expression in Sf9 cells
-
expression of GST-sAC fusion protein in SF9 cells
-
expression of isoenzymes ACI-ACVIII in COS cells
-
expression of isozyme ACVI in Spodoptera frugiperda Sf9 cell membranes, with a combination of four recombinant baculoviruses individually encoding the dopamine D1 receptor, G-protein alphaS-subunit, G-protein beta1gamma2 subunit dimer, and adenylyl cyclase type VI, ACVI. The recovered budded virus fraction produces cAMP in response to the stimulation with dopamine. Co-expression of all three G-protein subunits inaddition to receptor and ACVI led to amaximal response. Budded virusses co-expressingthel proteins also respond to dopamine agonists fenoldopam and SKF38393 and the antagonist flupenthixol, overview
expression of transmembrane isozymes in HEK293T cells, expression of GST-tagged soluble isozyme
HEK-293 cells stably transfected with AC9
-
ligated into pGEM-T Easy vector and expanded in DH5alpha Escherichia coli
-
polymorphism genotyping, and ADRB2 and ADCY6 gene interaction. The effect of ADRB2 on adhesion of erythrocytes in the sickle cell disease is different depending on the genotype of ADCY6. Sickle red cells from patients who were homozygous for either the G allele at single nucleotide polymorphism rs3730070 of ADCY6 demonstrate significantly increased adhesion to laminin compared to red cells from control patients, overview
-
the ADCY10 gene is located in the region linked to spinal bone mineral density
-
transient expression of fluorescent EGFP-tagged AC6 truncation mutants, expression of isozyme AC6 in COS-7 cells, expressing caveolin-1, and in HEK-293 cells or cardiac fibroblasts isolated from caveolin-1 knock-out mice, localization of AC6 in lipid rafts in all cell types, association via the AC6 C-terminal domains, expression analysis, overview
-
transient expression of tagged isozyme AC3 in HEK-PR1 cells, AC6 forms complexes with the IP3 R2 receptor; transient expression of tagged isozyme AC6 in HEK-PR1 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in SK-N-SH human neuroblastoma cells, morphine significantly increases RNA transcript and protein levels of type I adenylate cyclase
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F1078S
-
isoform VI, mutation within the GALPHAS binding pocket
N1090D
-
isoform V, mutation within the GALPHAS binding pocket
N955A
-
mutation prevents glycosylation
N964A
-
mutation prevents glycosylation
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
additional information