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H8F
little effect on the activity, but slower turnover of dibromomethane than wild-type
H8L
little effect on the activity, but slower turnover of dibromomethane than wild-type
H8Y
little effect on the activity, but slower turnover of dibromomethane than wild-type
S12A
drastically reduced activity
S12T
drastically reduced activity
V18A
lower affinity for GSH and slower turnover of dichlormethane than wild-type
V18I
lower affinity for GSH and slower turnover of dichlormethane than wild-type
V18T
lower affinity for GSH and slower turnover of dichlormethane than wild-type
W117Y
higher KM and lower specific activity for all tested substrates, better heat stability than wild-type
Y6A
little effect on the activity
Y6F
little effect on the activity
H8F
-
little effect on the activity, but slower turnover of dibromomethane than wild-type
-
H8L
-
little effect on the activity, but slower turnover of dibromomethane than wild-type
-
S12A
-
drastically reduced activity
-
V18A
-
lower affinity for GSH and slower turnover of dichlormethane than wild-type
-
V18I
-
lower affinity for GSH and slower turnover of dichlormethane than wild-type
-
V18T
-
lower affinity for GSH and slower turnover of dichlormethane than wild-type
-
W117Y
-
higher KM and lower specific activity for all tested substrates, better heat stability than wild-type
-
Y6A
-
little effect on the activity
-
Y6F
-
little effect on the activity
-
additional information
generation of a METDI2657 gene knockout mutant strain NOK353 from Methylobacterium dichloromethanicum wild-type strain DM4 using the mobilized suicide vector pK18mob, the mutant strain shows reduced growth on methanol compared to the wild-type enzyme
additional information
generation of a METDI2657 gene knockout mutant strain NOK353 from Methylobacterium dichloromethanicum wild-type strain DM4 using the mobilized suicide vector pK18mob, the mutant strain shows reduced growth on methanol compared to the wild-type enzyme
additional information
generation of a METDI2657 gene knockout mutant strain NOK353 from Methylobacterium dichloromethanicum wild-type strain DM4 using the mobilized suicide vector pK18mob, the mutant strain shows reduced growth on methanol compared to the wild-type enzyme
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