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EC unknown
Synonyms PIIN_06735 ,
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Agr5
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PIIN_06735
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(2E,6E)-farnesyl diphosphate + H2O = viridiflorol + diphosphate
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(2E,6E)-farnesyl-diphosphate diphosphate-lyase (viridiflorol-forming)
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(2E,6E)-farnesyl diphosphate + H2O
viridiflorol + diphosphate
(2E,6E)-farnesyl diphosphate + H2O
viridiflorol + viridiflorene + diphosphate
Substrates: -
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additional information
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(2E,6E)-farnesyl diphosphate + H2O
viridiflorol + diphosphate
Substrates: -
?
(2E,6E)-farnesyl diphosphate + H2O
viridiflorol + diphosphate
Substrates: -
?
(2E,6E)-farnesyl diphosphate + H2O
viridiflorol + diphosphate
Substrates: -
?
additional information
?
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Substrates: no substrates: geranyl diphosphate, and geranylgeranyl diphosphate
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additional information
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Substrates: no substrates: geranyl diphosphate, and geranylgeranyl diphosphate
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UniProt
brenda
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UniProt
brenda
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SwissProt
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isoform Agr5, cf. EC 4.2.3.8
SwissProt
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Highest Expressing Human Cell Lines
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physiological function
overexpression of enzyme does not lead to improved fungal colonization ability
physiological function
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overexpression of enzyme does not lead to improved fungal colonization ability
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AGR5_CYCAE
430
0
48034
Swiss-Prot
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G4TNA6_SERID
Serendipita indica (strain DSM 11827)
515
0
57085
TrEMBL
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homology modeling of structure and docking of Mg2+ and diphosphate
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G227C/E267S/V314Y
mutations improve viridiflorol production by 52%
G227C/V314Y
mutations improve viridiflorol production by 38%
G227C/V314Y/M318V
mutations reduce viridiflorol production by 90%
L174F
mutation reduces viridiflorol production by more than 95%
L174I
mutation reduces viridiflorol production by more than 95%
L174M
mutation reduces viridiflorol production by more than 95%. L174 is positioned in vicinity of magnesium binding motif DEYTD
L174T
mutation reduces viridiflorol production by more than 95%
L174V
mutation reduces viridiflorol production by more than 95%
L174Y
mutation reduces viridiflorol production by more than 95%
V314Y
mutation reduces viridiflorol production by more than 50%
additional information
removal of amino acids 2-85 from the N-terminus, i.e. the N-terminal signal peptide, of the wild-type and the double mutant G227C/V314Y markedly increases the viridiflorol titres and specific yields by about 200% and 100% for the wild-type and the mutant, respectively
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expression in Escherichia coli
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synthesis
production of viridiflorol in recombinant Escherichia coli via modification of the mevalonate and viridiflorol pathways at both transcriptional and translational levels. The best strain achieves 25.7 g/l and a yield of 0.22 g viridiflorol per g glucose in 2.5 days
agriculture
sesquiterpene synthase TPS and its products may provide Serendipita indica with a competitive advantage over other plant-associated fungi during root colonization. Viridiflorol has antifungal properties, addition of viridiflorol to the culture medium inhibits the germination of spores from a phytopathogenic fungus
agriculture
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sesquiterpene synthase TPS and its products may provide Serendipita indica with a competitive advantage over other plant-associated fungi during root colonization. Viridiflorol has antifungal properties, addition of viridiflorol to the culture medium inhibits the germination of spores from a phytopathogenic fungus
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Zhang, C.; Chen, X.; Orban, A.; Shukal, S.; Birk, F.; Too, H.; Ruehl, M.
Agrocybe aegerita serves as a gateway for identifying sesquiterpene biosynthetic enzymes in higher fungi
ACS Chem. Biol.
15
1268-1277
2020
Cyclocybe aegerita (A0A5Q0QSI8)
brenda
Ntana, F.; Bhat, W.W.; Johnson, S.R.; J rgensen, H.J.L.; Collinge, D.B.; Jensen, B.; Hamberger, B.
A sesquiterpene synthase from the endophytic fungus Serendipita indica catalyzes formation of viridiflorol
Biomolecules
11
898
2021
Serendipita indica (G4TNA6), Serendipita indica DSM 11827 (G4TNA6)
brenda
Shukal, S.; Chen, X.; Zhang, C.
Systematic engineering for high-yield production of viridiflorol and amorphadiene in auxotrophic Escherichia coli
Metab. Eng.
55
170-178
2019
Cyclocybe aegerita (A0A5Q0QSI8)
brenda
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