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IUBMB Comments Requires Mg2+ Streptomyces sp. SANK 60404. This trifunctional enzyme, which contains a [4Fe-4S]R )-nephthenol and (R )-cembrene A. See EC 4.2.3.150 , cembrene A synthase and EC 4.2.3.149 , nephthenol synthase.
Synonyms DtcycB ,
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DtcycB
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DtcycB
gene name. The trifunctional enzyme also produces (R)-nephthenol and (R)-cembrene A. See EC 4.2.3.150, cembrene A synthase and EC 4.2.3.149, nephthenol synthase
DtcycB
gene name. The trifunctional enzyme also produces (R)-nephthenol and (R)-cembrene A. See EC 4.2.3.150, cembrene A synthase and EC 4.2.3.149, nephthenol synthase
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geranylgeranyl diphosphate + H2O = (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
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geranylgeranyl-diphosphate diphosphate-lyase [(1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol-forming]
Requires Mg2+. Isolated from the bacterium Streptomyces sp. SANK 60404. This trifunctional enzyme, which contains a [4Fe-4S] cluster, also produces (R)-nephthenol and (R)-cembrene A. See EC 4.2.3.150, cembrene A synthase and EC 4.2.3.149, nephthenol synthase.
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geranylgeranyl diphosphate
(1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
geranylgeranyl diphosphate
(1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
Substrates: -
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geranylgeranyl diphosphate
(1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
Substrates: the trifunctional enzyme also produces (R)-nephthenol and (R)-cembrene A. See EC 4.2.3.150, cembrene A synthase and EC 4.2.3.149, nephthenol synthase
?
geranylgeranyl diphosphate
(1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
Substrates: -
?
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geranylgeranyl diphosphate
(1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
geranylgeranyl diphosphate
(1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
Substrates: -
?
geranylgeranyl diphosphate
(1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
Substrates: -
?
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0.0421
geranylgeranyl diphosphate
pH 7.5, 30°C
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0.022
geranylgeranyl diphosphate
pH 7.5, 30°C
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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DTCYB_STRSQ
343
0
37887
Swiss-Prot
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38000
2 * 38000, SDS-PAGE
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homodimer
2 * 38000, SDS-PAGE
homodimer
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2 * 38000, SDS-PAGE
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overexpression in Escherichia coli
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Meguro, A.; Tomita, T.; Nishiyama, M.; Kuzuyama, T.
Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton
ChemBioChem
14
316-321
2013
Streptomyces sp. (M1VDX3), Streptomyces sp. SANK 60404 (M1VDX3)
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