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EC Tree
IUBMB Comments Isolated from the plant Phyla dulcis (Aztec sweet herb). (+)-epi-alpha-Bisabolol is the precursor of the sweetener hernandulcin.
The enzyme appears in viruses and cellular organisms
Synonyms
(+)-epi-alpha-bisabolol synthase, LdTPS8, TPS8,
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(+)-epi-alpha-bisabolol synthase
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(2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate
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(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-epi-alpha-bisabolol-forming)
Isolated from the plant Phyla dulcis (Aztec sweet herb). (+)-epi-alpha-Bisabolol is the precursor of the sweetener hernandulcin.
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(2E,6E)-farnesyl diphosphate + H2O
(+)-epi-alpha-bisabolol + diphosphate
(2E,6E)-farnesyl diphosphate + H2O
(+)-epi-alpha-bisabolol + diphosphate
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(2E,6E)-farnesyl diphosphate + H2O
(+)-epi-alpha-bisabolol + diphosphate
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single terpene product in vitro
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?
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0.0048
(2E,6E)-farnesyl diphosphate
0.0048
(2E,6E)-farnesyl diphosphate
pH 7.5, 30°C
0.0048
(2E,6E)-farnesyl diphosphate
pH 7.4, 30°C
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0.04
(2E,6E)-farnesyl diphosphate
0.04
(2E,6E)-farnesyl diphosphate
pH 7.5, 30°C
0.04
(2E,6E)-farnesyl diphosphate
pH 7.4, 30°C
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UniProt
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TPS8_PHYDL
546
0
63782
Swiss-Prot
Mitochondrion (Reliability: 5 )
A0A2G9GJJ4_9LAMI
515
0
60751
TrEMBL
other Location (Reliability: 2 )
A0A2G9GJS1_9LAMI
543
0
63856
TrEMBL
other Location (Reliability: 5 )
A0A2G9GJH3_9LAMI
120
0
14231
TrEMBL
Secretory Pathway (Reliability: 5 )
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using Ni-NTA chromatography
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expressed in yeast and Escherichia coli. The engineered yeast can synthesize a significant amount (0.3 mg per ml) of alpha-bisabolol in shake-flask cultivation
expression in Saccharomyces cerevisiae and Escherichia coli
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Attia, M.; Kim, S.U.; Ro, D.K.
Molecular cloning and characterization of (+)-epi-alpha-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis
Arch. Biochem. Biophys.
527
37-44
2012
Phyla dulcis, Phyla dulcis (J7LH11)
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4.2.3.138 (+)-epi-alpha-bisabolol synthase
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