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Information on EC 4.2.3.106 - (E)-beta-ocimene synthase
for references in articles please use BRENDA:EC4.2.3.106
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EC Tree 4 Lyases
4.2 Carbon-oxygen lyases
4.2.3 Acting on phosphates
4.2.3.106 (E)-beta-ocimene synthase
IUBMB Comments
Widely distributed in plants, which release β-ocimene when attacked by herbivorous insects.
The enzyme appears in viruses and cellular organisms
- 4.2.3.106
-
terpene
-
terpenoids
-
e-beta-ocimene
-
monoterpene
-
floral
-
scent
- herbivory
- myrcene
-
z-3-hexenyl
- lunatus
-
guild
- 1-deoxy-d-xylulose
-
tetranychus
- reductoisomerase
-
insect-induced
-
plant-insect
-
enemies
-
bumblebee
-
urticae
- lewisii
- littoralis
-
gaertn
- mimulus
- agriculture
showSynonyms
attps03, ocimene synthase, ljebetaos, mtebos, jstps, beta-ocimene synthase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ama0a23
AtTPS03
-
-
-
-
beta-ocimene synthase
EbetaOS
EBOS
LjEbetaOS
-
-
-
-
MtEBOS
-
-
-
-
MTSL4
ocimene synthase
OCS
ama0a23
-
-
-
-
beta-ocimene synthase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = (E)-beta-ocimene + diphosphate
-
-
-
-
geranyl diphosphate = (E)-beta-ocimene + diphosphate
protein does not contain the RRx8W motif,suggesting that the reaction mechanism does not proceed via an RR-dependent isomerization of geranyl diphosphate to 3S-linalyl diphosphate
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [(E)-beta-ocimene-forming]
Widely distributed in plants, which release beta-ocimene when attacked by herbivorous insects.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + (Z)-beta-ocimene + myrcene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + (Z)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + (Z)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: products are 97% (E)-beta-ocimene plus 2% (Z)-beta-ocimene and 1% myrcene
Products: products are 97% (E)-beta-ocimene plus 2% (Z)-beta-ocimene and 1% myrcene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: product formation does not proceed via an RR-dependent isomerization of geranyl diphosphate to 3S-linalyl diphosphate
Products: 97% (E)-beta-ocimene plus minor amounts of (Z)-beta-ocimene and myrcene
Products: 97% (E)-beta-ocimene plus minor amounts of (Z)-beta-ocimene and myrcene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: products of Arabidopsis thaliana TPS10 are 56% beta-myrcene, 20% (E)-beta-ocimene, and less than 5% each of (+)-limonene, (-)-limonene, 2-carene, and tricyclene and an unknown monoterpene
Products: products of Arabidopsis thaliana TPS10 are 56% beta-myrcene, 20% (E)-beta-ocimene, and less than 5% each of (+)-limonene, (-)-limonene, 2-carene, and tricyclene and an unknown monoterpene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: main products are 56% beta-myrcene and 20% (E)-beta-ocimene plus minor amounts of (-)-limonene, (+)-limnonene, 2-carene and tricyclene
Products: main products are 56% beta-myrcene and 20% (E)-beta-ocimene plus minor amounts of (-)-limonene, (+)-limnonene, 2-carene and tricyclene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: 94% (E)-beta-ocimene plus 4% (Z)-beta-ocimene and 2% myrcene
Products: 94% (E)-beta-ocimene plus 4% (Z)-beta-ocimene and 2% myrcene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: 97.2% (E)-beta-ocimene
Products: 97.2% (E)-beta-ocimene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: 97.2% (E)-beta-ocimene
Products: 97.2% (E)-beta-ocimene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: products are alpha-thujene and (+)-sabinene
Products: products are alpha-thujene and (+)-sabinene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: products are 98% (E)-beta-ocimene plus 2% (Z)-beta-ocimene
Products: products are 98% (E)-beta-ocimene plus 2% (Z)-beta-ocimene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: 98% (E)-beta-ocimene plus 2% (Z)-beta-ocimene
Products: 98% (E)-beta-ocimene plus 2% (Z)-beta-ocimene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: products are 97.5% (E)-beta-ocimene plus 2% (Z)-beta-ocimene and 0.5% myrcene
Products: products are 97.5% (E)-beta-ocimene plus 2% (Z)-beta-ocimene and 0.5% myrcene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: 97.5% of (E)-beta-ocimene plus 2% (Z)-beta-ocimene and 0.5% myrcene
Products: 97.5% of (E)-beta-ocimene plus 2% (Z)-beta-ocimene and 0.5% myrcene
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme shows both (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme OCS can produce (E)-beta-ocimene and (Z)-beta-ocimene using geranyl diphosphate (GPP) as the substrate. No activity with farnesyl diphosphate as substrate. The activity and kinetics of CsOCS are investigated using a malachite green phosphate assay at pH 7.5, 30°C
Products: -
Products: -
-
additional information
?
-
Substrates: the enzyme shows no activity with farnesyl diphosphate. Reaction products are identified by GC-MS analysis
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: farnesyl diphosphate and geranylgeranyl diphosphate
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: farnesyl diphosphate and geranylgeranyl diphosphate
Products: -
Products: -
?
additional information
?
-
Substrates: in vivo emission of E-beta-ocimene from flowers is very low, but southern race Mimulus lewisii flowers emit significantly more E-beta-ocimene than northern race flowers
Products: -
Products: -
?
additional information
?
-
-
Substrates: in vivo emission of E-beta-ocimene from flowers is very low, but southern race Mimulus lewisii flowers emit significantly more E-beta-ocimene than northern race flowers
Products: -
Products: -
?
additional information
?
-
Substrates: GC-MS product analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: GC-MS product analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: recombinant GmOCS catalyzes geranyl diphosphate (GPP) mainly into (E)-beta-ocimene (94.27%), (Z)-ocimene (3.47%), and linalool (2.26%). No obvious products are observed when GmOCS is incubated with neryl diphosphate (NPP), (E,E)-farnesyl diphosphate ((E,E)-FPP), or (Z,Z)-farnesyl diphosphate ((Z,Z)-FPP). Identification of compounds by GC-MS analysis
Products: -
Products: -
-
additional information
?
-
Substrates: GC-MS analysis of floral volatiles and enzyme reaction product
Products: -
Products: -
?
additional information
?
-
-
Substrates: GC-MS analysis of floral volatiles and enzyme reaction product
Products: -
Products: -
?
additional information
?
-
Substrates: no substrates: farnesyl diphosphate or geranylgeranyl diphosphate.
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrates: farnesyl diphosphate or geranylgeranyl diphosphate.
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: farnesyl diphosphate
Products: -
Products: -
?
additional information
?
-
-
Substrates: the volatile products are trapped by solid phase microextraction (SPME) with polydimethylsiloxane (PDMS) fiber for 30 min at 30°C, and then analyzed by a GC-MS system. In enzyme-catalyzed reaction, recombinant enzyme PbeOCS utilizes only geranyl diphosphate but not neryl diphosphate, farnesyl diphosphate, or geranylgeranyl diphosphate as a substrate, producing (E)-beta-ocimene as the major product and a trace amount of (Z)-beta-ocimene
Products: -
Products: -
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
(E)-beta-ocimene + (Z)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
-
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
Substrates: -
Products: -
Products: -
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: in vivo emission of E-beta-ocimene from flowers is very low, but southern race Mimulus lewisii flowers emit significantly more E-beta-ocimene than northern race flowers
Products: -
Products: -
?
additional information
?
-
-
Substrates: in vivo emission of E-beta-ocimene from flowers is very low, but southern race Mimulus lewisii flowers emit significantly more E-beta-ocimene than northern race flowers
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
additional information
-
the enzyme sequence contains the Mg2+ or Mn2+ cofactor-binding-related DDXX (D/E) and (N,D) DXX (S,T,G) XXXE (NSE/DTE) motifs
Mg2+
required, the enzyme sequence has two Mg2+ binding sites (aspartate-rich motif) DDXXD and NDLXTSXXE motifs
Mg2+
sequence has two Mg2+ binding sites, DDXXD and NDLXTSXXE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0026
(2E,6E)-farnesyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
additional information
additional information
Michaelis-Menten kinetics
-
0.0067
geranyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0048
(2E,6E)-farnesyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
0.034
geranyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.82
(2E,6E)-farnesyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
5.12
geranyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 8.5
the enzyme activity dramatically reduces below pH 7.0 or above pH 8.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25 - 35
25 - 35
at 25°C or 35°C, the enzyme activity drops to below 65% of the initial level compared to optimal 30°C
25 - 35
at 25 or 35°C, the enzyme activity drops to below 65% of the initial level compared to 30°C
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Erythranthe lewisii, southern race distributed in the Sierra Nevada
UniProt
brenda
deduced protein does not contain the RRx8W motif; member of a subfamily of the terpene synthase family designated the Tps-g group, which lacks the RRx8W motif
UniProt
brenda
member of TPSb group of angiosperm monoterpene and hemiterpene synthases
UniProt
brenda
member oft the TPSb subfamily of angiosperm monoterpene synthases
SwissProt
brenda
the tandem arginine motif RRX8W is conserved in the deduced protein of TPS10; bifunctional myrcene/(E)-beta-ocimene synthase
SwissProt
brenda
var. Tieguanyin, leaves are collectd on Juyuan Tea Farm at Anxi county (Quanzhou, Fujian Province, China) (altitude: 810 m, 24°57'22'' N, 117°49' E) in October 2017
UniProt
brenda
deduced protein contains a modification of the conserved RRx8W motif of plant monoterpene synthases. The N-terminal sequence indicates the presence of a plastid transit peptide
UniProt
brenda
member of the Tps-b subfamily containing a RRx8W motif
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
abundant in flower, amount decreases towards fruit development
brenda
-
quantitative RT-PCR analysis shows that HcTPS3 is highly expressed only in flower tissue, likewise, the accumulation of beta-ocimene is highest in Hedychium coronarium flower
brenda
abundant in flower, amount decreases towards fruit development
brenda
(E)-b-ocimene is released from leaves of both undamaged and insect-damaged plants, but at levels two-fold higher in insect-damaged M.edicago truncatula
brenda
maximum TPS transcript accumulation is observed in flower petals, analysis of JsTPS expression in different developmental stages and in different floral part by semiquantitative RT-PCR. Unopened flower (buds) emit no beta-ocimene or farnesene. Senescence initiated in Jasminnum sambac flower after 24 h of petal opening, while low emission of linalool and beta-ocimene is detected until the abscission of floral tissue. beta-Ocimene is not detected in any other floral tissue except petals with a very lowamount of emission
brenda
additional information
transcriptome analysis of trichomes of the cannabis hemp variety Finola reveals sequences of all stages of terpene biosynthesis. Major terpene compounds such as beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene. Transcripts associated with terpene biosynthesis are highly expressed in trichomes compared to non-resin producing tissues. Relative composition of terpene profiles in Cannabis sativa var. Finola pistillate flowers, overview
brenda
additional information
-
transcription factor HcARF5 shows a higher expression level in flowers, and significantly correlates with the key structural beta-ocimene synthesis gene, HcTPS3. Transcript levels of both genes are associated with the flower development
brenda
additional information
during the life span of Jasminum sambac flower, clear variations in monoterpene emission between the day and night periods are observed for linalool and beta-ocimene that continue to emituntil next morning
brenda
additional information
-
during the life span of Jasminum sambac flower, clear variations in monoterpene emission between the day and night periods are observed for linalool and beta-ocimene that continue to emituntil next morning
brenda
additional information
no constitutive expression in flowers, stems or roots
brenda
additional information
transcripts of MtEBOS are not detected via RNA blots in stems, flowers, and roots
brenda
additional information
-
transcripts of MtEBOS are not detected via RNA blots in stems, flowers, and roots
brenda
additional information
-
transcriptome analysis of non-infected and Spodoptera litura-infected seedlings, overview
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
N-terminal sequence indicates the presence of a plastid transit peptide
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
-
the overexpression of GmOCS in soybean plants leads to enhanced resistance against Spodoptera litura
metabolism
transcriptome analysis of trichomes of the cannabis hemp variety Finola reveal sequences of all stages of terpene biosynthesis. Nine cannabis terpene synthases (CsTPS) are identified in subfamilies TPS-a and TPS-b. Functional characterization identified mono- and sesqui-TPS, whose products collectively comprise most of the terpenes of Finola resin, including major compounds such as beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene
physiological function
additional information
evolution
the enzyme is a member of the terpene synthase (TPS) superfamily, CsTPSFN TPS-b subfamily
evolution
the taxa closely related to Mimulus lewisii have evolved several diff erent pollination syndromes, including hummingbird pollination and self pollination (autogamy), floral scent variation contributing to species diversification in this clade, E-beta-ocimene emission within this Mimulus clade does only occur in Mimulus lewisii, given the very low E-beta-ocimene emission in vivo, Mimulus parishii has a fully functional ocimene synthase enzyme in vitro, the other taxa do not emit E-beta- ocimene from flowers, overview
evolution
-
phylogeny, gene structure, conservative motif, gene location, and expression patterns of GmTPS genes, overview
evolution
CsOCS is clustered into the TPS-b subclade, which belongs to the angiosperm monoterpene synthase subfamily. Within this subclade, CsOCS falls into a monophyletic group (the so-called isoprene/ocimene synthase clade), which comprises members encoding enzymes to produce beta-ocimene, myrcene, and isoprene
physiological function
differences in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from accession Wassilewskija, a high-(E)-beta-ocimene emitter, and accession Columbia, a trace-(E)-beta-ocimene emitter, are attributed to allelic variation of closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. The Wassilewskija genome contains a functional allele of TPS02 but not of TPS03, while the opposite is the case for Columbia. Recombinant proteins of the functional Wassilewskija TPS02 and Columbia TPS03 genes both show (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities. Differential subcellular compartmentalization of the two enzymes in plastids and the cytosol is responsible for the ecotype-specific differences in (E)-beta-ocimene/(E,E)-alpha-farnesene emission
physiological function
enzyme is involved in the herbivore-induced indirect defense response of spider mite-infested Lotus japonicus via de novo formation and emission (E)-beta-ocimene
physiological function
floral scent of Jasminum sambac (Oleaceae) includes three major benzenoid esters: benzylacetate, methylbenzoate, and methylsalicylate, and three major terpene compounds viz. (E)-beta-ocimene, linalool and alpha-farnesene, analysis of concentrations and emission rates of benzenoids and terpenoids during the developmental stages of the flowers, overview
physiological function
E-beta-ocimene, a monoterpene produced by ocimene synthase (OS) in Mimulus lewisii, is a floral scent important in attracting the species' bumblebee pollinators
physiological function
-
the upregulation of PbeOCS in Spodoptera litura-infested pear leaves supports a potential role for PbeOCS in herbivore-induced plant defenses. (E)-beta-Ocimene shows repellent effects on larvae of Spodoptera litura in dual-choice bioassays and increases mortalities of larvae in no-choice bioassays
physiological function
-
plastidic localized monoterpene synthase gene GmOCS is responsible for the biosynthesis of (E)-beta-ocimene. (E)-beta-Ocimene synthase gene contributes to the defense against the herbivorous insect Spodoptera litura, (E)-beta-ocimene shows anti-insect function in soybean, it has a pivotal role in repelling a Spodoptera litura attack, mechanisms underlying defense responses of soybean against pests, overview
physiological function
as the major contributors to the floral odors of tea products, terpenoid volatiles play critical roles in the defense response of plants to multiple stresses. Stress-induced beta-ocimene biosynthesis in tea leaves during Oolong tea processing. CsOCS is implicated as a key enzyme for beta-ocimene synthesis during oolong tea processing, importance of ocimene for tea flavors and for inter-/intraplant communications
physiological function
-
beta-ocimene is a key floral volatile compound synthesized by terpene synthase 3 (HcTPS3) in Hedychium coronarium. Auxin response factor (ARF) transcription factors reveal HcARF5 expression profile associated with the biosynthesis of beta-ocimene synthase in Hedychium coronarium. HcARF5 localizes to the nucleus and possesses transcriptional activity, and HcARF5 directly regulates the transcriptional activity of HcTPS3. HcARF5 activates the beta-ocimene synthase (HcTPS3) expression
physiological function
key enzyme for beta-ocimene synthesis during oolong tea processing
additional information
-
the PbeOCS protein has conserved structural features such as the DDxxD motif, the NSE/DTE motif, the RS(X)8Wmotif in the N-terminal region, and the arginine-rich RxR motif located at 35 amino acids upstream of the DDxxD motif, all of which are highly conserved in TPSs in plants
additional information
-
the enzyme contains the terpene-cyclization-related RR(X)8W motif and the Mg2+ or Mn2+ cofactor-binding-related DDXX (D/E) and (N,D) DXX (S,T,G) XXXE (NSE/DTE) motifs
additional information
the enzyme sequence has two Mg2+ binding sites (aspartate-rich motif) DDXXD and NDLXTSXXE motifs
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). TPS1_MELLP
Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7)
324
0
36910
Swiss-Prot
-
TPS2_MELLI
324
0
36757
Swiss-Prot
other Location (Reliability: 2)
BOMS_ORIVU
595
0
69381
Swiss-Prot
other Location (Reliability: 2)
TPS1_LITCU
582
0
67150
Swiss-Prot
other Location (Reliability: 4)
TPS4_MATCR
596
0
69526
Swiss-Prot
other Location (Reliability: 2)
TPS1_DICDI
345
0
40595
Swiss-Prot
other Location (Reliability: 1)
A0A072TWP0_MEDTR
560
0
64990
TrEMBL
other Location (Reliability: 2)
A0A072UIV0_MEDTR
470
0
54805
TrEMBL
other Location (Reliability: 2)
A0A072UIS6_MEDTR
580
0
67133
TrEMBL
Mitochondrion (Reliability: 4)
A0A1S2XFW4_CICAR
594
0
70605
TrEMBL
Secretory Pathway (Reliability: 2)
A0A1S2XM19_CICAR
556
0
64398
TrEMBL
other Location (Reliability: 3)
A0A1S2Z458_CICAR
556
0
64324
TrEMBL
other Location (Reliability: 2)
A0A396GXV1_MEDTR
498
0
58202
TrEMBL
other Location (Reliability: 2)
A0A396I3X8_MEDTR
561
0
65421
TrEMBL
Secretory Pathway (Reliability: 1)
A0A7T0M889_LATOD
560
0
64982
TrEMBL
Secretory Pathway (Reliability: 1)
A0A9D4VPG0_PEA
466
0
54175
TrEMBL
Mitochondrion (Reliability: 4)
A0A9D4Y9R0_PEA
557
0
64799
TrEMBL
Mitochondrion (Reliability: 3)
A0A9D4YE81_PEA
458
0
53727
TrEMBL
Mitochondrion (Reliability: 4)
A0A9D4YNS7_PEA
548
0
64911
TrEMBL
other Location (Reliability: 1)
A0A9D5A7H2_PEA
534
0
62993
TrEMBL
other Location (Reliability: 2)
A0AAV0YNY8_VICFA
556
0
65085
TrEMBL
other Location (Reliability: 2)
A0AAV0YNN0_VICFA
589
0
69317
TrEMBL
Secretory Pathway (Reliability: 3)
G7KA52_MEDTR
553
0
64426
TrEMBL
Secretory Pathway (Reliability: 1)
G7JZP8_MEDTR
552
0
64531
TrEMBL
other Location (Reliability: 1)
G7KJE7_MEDTR
557
0
65036
TrEMBL
other Location (Reliability: 3)
A0A4S4EKW4_CAMSI
248
0
27832
TrEMBL
-
TPS1_ANTMA
579
0
66728
Swiss-Prot
other Location (Reliability: 4)
TPSA_ARATH
591
0
69279
Swiss-Prot
Secretory Pathway (Reliability: 1)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
the overexpression of GmOCS in soybean plants leads to enhanced resistance against Spodoptera litura. GmOCS is a good candidate in anti-herbivore plant modification
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant MBP-tagged enzyme from Escherichia coli strain Rosetta(DE3) by amylose affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta (DE3) cells and in Nicotiana benthamiana leaves
expression in Escherichia coli
gene CsOCS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of MBP-tagged enzyme in Escherichia coli strain Rosetta(DE3), transient recombinant overexpression of enzyme CsOCS in Nicotiana benthamiana leaves via Agrobacterium tumefaciens strain GV3101-mediated transfection method under control of the CaMV S35 promoter, CsOCS is targeted to the plastid. Quantitative real-time PCR enzyme expression analysis
gene CsTPS6FN, sequence comparisons and phylogenetic analysis, RT-PCR expression analysis
gene HcTPS3, quantitative real-time PCR enzyme expression analysis. HcARF5 localizes to the nucleus and possesses transcriptional activity, and HcARF5 directly regulates the transcriptional activity of HcTPS3. HcARF5 activates the beta-ocimene synthase (HcTPS3) expression. Analysis of gene structure, motif prediction, cis-regulatory elements and HcARF targeting miRNAs. Recombinant co-expression of HcTPS3 and HcARF5 in Nicotiana benthamiana leaves via Agrobacterium tumefaciens strain EH105-mediated transfection method, interaction analysis by bimolecular fluorescence complementation (BiFC) assay
-
gene OCS or TPS3, DNA and amino acid sequence determination and analysis, analysis of the acting regulatory elements in promoter sequences of GmOCS, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana protoplasts in the chloroplasts. Transient overexpression of enzyme GmOCS in Nicotiana benthamiana leaves via Agrobacterium tumefaciens strain GV3101-mediated transfection method. The overexpression of GmOCS in soybean plants leads to enhanced resistance against Spodoptera litura
-
gene OCS, transcriptome analysis, cloning, and quantitative PCR enzyme expression analysis, sequence comparisons and phylogenetic analysis and tree, recombinant enzyme expression in Escherichia coli strain BL21 (DE3)
-
gene OS, genotyping and genomic analysis, sequence comparisons, quantitative RT-PCR expression analysis, recombinant overexpression in Escherichia coli strain BL21
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme GmOCS is strongly induced by methyljasmonate and Spodoptera litura attack, but not by mechanical wounding. The transcripts of GmOCS continuously increase with a prolonged attack by Spodoptera litura
-
induced in plants infested with two-spotted spider mites (Tetranychus urticae), coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. Mechanical wounding of the leaves or application of alamethicin also induces transiently increased levels of EbOS transcripts in Lotus japonicus. Wounding or alamethicin does not result in elevated release of (E)-beta-ocimene
mRNA is detected first in mature flower buds, and its level increases until it peaks on day 4 after anthesis. Over the next 3 days, mRNA levels decline sharply by 40% and decrease slowly thereafter
only slight variations in monoterpene synthase mRNA levels during the daily light/dark cycle
stress-induced beta-ocimene biosynthesis in tea leaves during Oolong tea processing. Treatment with exogenous methyl jasmonate amd jasmonate elevate the transcript level of CsOCS and enhance the emission of ocimene from tea leaves
transcripts accumulate in leaves in response to exogenous jasmonic acid treatments, lepidopteran herbivory, and lepidopteran oral secretions
transcripts are upregulated in response to mechanical wounding and treatment with jasmonic acid, concurrent with induced emission of (E)-beta-ocimene
transcripts of LjEbOS are induced in plants infested with two-spotted spider mites, Tetranychus urticae, coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. Mechanical wounding of the leaves or application of alamethicin, a potent fungal elicitor of plant volatile emission, also induces transiently increased levels of transcripts, but not elevated release of (E)-beta-ocimene
treatment with 1-aminocyclopropane-1-carboxylic acid does not cause an increase in transcripts
treatment with exogenous methyl jasmonate elevates the transcript level of the enzyme
treatment with the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid does not cause an increase in transcripts
transcripts accumulate in leaves in response to exogenous jasmonic acid treatments, lepidopteran herbivory, and lepidopteran oral secretions
transcripts accumulate in leaves in response to exogenous jasmonic acid treatments, lepidopteran herbivory, and lepidopteran oral secretions
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
when lima bean plants that have previously been placed downwind of transgenic tobacco plants that are constitutively overexpressing (E)-beta-ocimene synthase are infested by spider mites in an open-flow tunnel, they are more defensive to spider mites and more attractive to predatory mites, in comparison to the infested plants that have been placed downwind of wild-type tobacco plants. Similarly, when the transgenic tobacco-downwind maize plants are infested with Mythimna separata larvae, this results in reduced larval growth and greater attraction of parasitic wasps Cotesia kariyai. In a greenhouse experiment, lima bean plants placed near the transgenic tobacco plants are more attractive when damaged by spider mites, in comparison to the infested plants that have been placed near the wild-type plants. Volatile organic compounds emitted from infested receiver plants affect their conspecific neighboring plants to prime indirect defenses in response to herbivory
food industry
CsOCS is implicated as a key enzyme for beta-ocimene synthesis during oolong tea processing. Manufacturing process of Tieguanyin oolong tea in and the changes of ocimene contents, overview
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bohlmann, J.; Martin, D.; Oldham, N.J.; Gershenzon, J.
Terpenoid secondary metabolism in Arabidopsis thaliana: cDNA cloning, characterization, and functional expression of a myrcene/(E)-beta-ocimene synthase
Arch. Biochem. Biophys.
375
261-269
2000
Arabidopsis thaliana (Q9ZUH4), Arabidopsis thaliana
brenda
Dudareva, N.; Martin, D.; Kish, C.M.; Kolosova, N.; Gorenstein, N.; Fldt, J.; Miller, B.; Bohlmann, J.
(E)-beta-Ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon: function and expression of three terpene synthase genes of a new terpene synthase subfamily
Plant Cell
15
1227-1241
2003
Antirrhinum majus (Q84NC8)
brenda
Navia-Gine, W.G.; Yuan, J.S.; Mauromoustakos, A.; Murphy, J.B.; Chen, F.; Korth, K.L.
Medicago truncatula (E)-beta-ocimene synthase is induced by insect herbivory with corresponding increases in emission of volatile ocimene
Plant Physiol. Biochem.
47
416425
2009
Medicago truncatula (Q5UB07)
-
brenda
Arimura, G.; Ozawa, R.; Kugimiya, S.; Takabayashi, J;, Bohlmann, J.
Herbivore-induced defense response in a model legume. Two-spotted spider mites induce emission of (E)-beta-ocimene and transcript accumulation of (E)-beta-ocimene synthase in Lotus japonicus
Plant Physiol.
135
1976-1983
2004
Lotus japonicus (Q672F7), Lotus japonicus
brenda
Huang, M.; Abel, C.; Sohrabi, R.; Petri, J.; Haupt, I.; Cosimano, J.; Gershenzon, J.; Tholl, D.
Variation of herbivore-induced volatile terpenes among Arabidopsis ecotypes depends on allelic differences and subcellular targeting of two terpene synthases, TPS02 and TPS03
Plant Physiol.
153
1293-1310
2010
Arabidopsis thaliana (A4FVP2)
brenda
Navia-Gine, W.G.; Yuan, J.S.; Mauromoustakos, A.; Murphy, J.B.; Chen, F.; Korth, K.L.
Medicago truncatula (E)-beta-ocimene synthase is induced by insect herbivory with corresponding increases in emission of volatile ocimene
Plant Physiol. Biochem.
47
416-425
2009
Medicago truncatula (Q5UB07), Medicago truncatula
brenda
Shimada, T.; Endo, T.; Fujii, H.; Hara, M.; Omura, M.
Isolation and characterization of (E)-beta-ocimene and 1,8 cineole synthases in Citrus unshiu Marc
Plant Sci.
168
987-995
2005
Citrus unshiu (Q5CD81), Citrus unshiu Marc (Q5CD81)
-
brenda
Faeldt, J.; Arimura, G.; Gershenzon, J.; Takabayashi, J.; Bohlmann, J.
Functional identification of AtTPS03 as (E)-beta-ocimene synthase: a monoterpene synthase catalyzing jasmonate- and wound-induced volatile formation in Arabidopsis thaliana
Planta
216
745-751
2003
Arabidopsis thaliana (A4FVP2), Arabidopsis thaliana
brenda
Chang, Y.; Chu, F.
Molecular cloning and characterization of monoterpene synthases from Litsea cubeba (Lour.) Persoon
Tree Genet. Genomes
7
835-844
2011
Litsea cubeba (G0Y7D1)
-
brenda
Muroi, A.; Ramadan, A.; Nishihara, M.; Yamamoto, M.; Ozawa, R.; Takabayashi, J.; Arimura, G.
The composite effect of transgenic plant volatiles for acquired immunity to herbivory caused by inter-plant communications
PLoS ONE
6
e24594
2011
Phaseolus lunatus (B1P189)
brenda
Peng, F.; Byers, K.; Bradshaw, H.J.
Less is more Independent loss-of-function ocimene synthase alleles parallel pollination syndrome diversification in monkeyflowers (Mimulus)
Am. J. Bot.
104
1055-1059
2017
Erythranthe lewisii (W6A3F2), Erythranthe lewisii
brenda
Bera, P.; Mukherjee, C.; Mitra, A.
Enzymatic production and emission of floral scent volatiles in Jasminum sambac
Plant Sci.
256
25-38
2017
Jasminum sambac (A0A1B0WVE7), Jasminum sambac
brenda
Booth, J.; Page, J.; Bohlmann, J.
Terpene synthases from Cannabis sativa
PLoS ONE
12
e0173911
2017
Cannabis sativa (A0A1V0QSH1)
brenda
Huang, X.; Zhang, H.; Li, H.; Wang, M.; Guo, X.; Liu, E.; Han, X.; Zhen, C.; Li, A.; Shi, W.; Zhang, Y.
Functional characterization of a terpene synthase responsible for (E)-beta-ocimene biosynthesis identified in Pyrus betuleafolia transcriptome after herbivory
Front. Plant Sci.
13
1077229
2022
Pyrus betulifolia
brenda
Han, J.; Li, T.; Wang, X.; Zhang, X.; Bai, X.; Shao, H.; Wang, S.; Hu, Z.; Wu, J.; Leng, P.
AmMYB24 regulates floral terpenoid biosynthesis induced by blue light in snapdragon flowers
Front. Plant Sci.
13
885168
2022
Antirrhinum majus
brenda
Han, T.; Shao, Y.; Gao, R.; Gao, J.; Jiang, Y.; Yang, Y.; Wang, Y.; Yang, S.; Gao, X.; Wang, L.; Li, Y.
Functional characterization of a (E)-beta-ocimene synthase gene contributing to the defense against Spodoptera litura
Int. J. Mol. Sci.
24
7182
2023
Glycine max
brenda
Chen, S.; Xie, P.; Li, Y.; Wang, X.; Liu, H.; Wang, S.; Han, W.; Wu, R.; Li, X.; Guan, Y.; Yang, Z.; Yu, X.
New insights into stress-induced beta-ocimene biosynthesis in tea (Camellia sinensis) leaves during Oolong tea processing
J. Agric. Food Chem.
69
11656-11664
2021
Camellia sinensis (A0A6G6A9V1), Camellia sinensis var. sinensis (A0A4S4EKW4)
brenda
Abbas, F.; Ke, Y.; Zhou, Y.; Yu, Y.; Waseem, M.; Ashraf, U.; Li, X.; Yu, R.; Fan, Y.
Genome-wide analysis of ARF transcription factors reveals HcARF5 expression profile associated with the biosynthesis of beta-ocimene synthase in Hedychium coronarium
Plant Cell Rep.
40
1269-1284
2021
Hedychium coronarium
brenda
Lee, G.W.; Chung, M.S.; Lee, S.S.; Chung, B.Y.; Lee, S.
Transcriptome-guided identification and functional characterization of key terpene synthases involved in constitutive and methyl jasmonate-inducible volatile terpene formation in Eremochloa ophiuroides (Munro) Hack
Plant physiol. Biochem.
141
193-201
2019
Eremochloa ophiuroides
brenda
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