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Enzyme Nomenclature
Information on EC 4.2.1.69 - cyanamide hydratase
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4.2.1.69 cyanamide hydrataseSpecify your search results
Word Map
- 4.2.1.69
-
detoxify
-
myrothecium
-
broad-spectrum
-
cyanuric
- verrucaria
-
untransformed
-
disinfection
- agriculture
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
cyanamide-hydratase
-
-
-
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hydratase, cyanamide
-
-
-
-
Urea hydro-lyase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
urea = cyanamide + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydration
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
urea hydro-lyase (cyanamide-forming)
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CAS REGISTRY NUMBER
COMMENTARY
50812-20-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cyanamide + H2O
?
-
no constitutive enzyme, induced by addition of cyanamide to growth medium
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-
?
additional information
?
-
-
not: Arg, Orn, urea, guanidine, guanidino-urea, creatin, creatinin, guanidinoacetate
-
-
?
additional information
?
-
-
cyanamide hydratase (cah) gene used as marker system for regeneration of transgenic plants
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cyanamide + H2O
?
-
no constitutive enzyme, induced by addition of cyanamide to growth medium
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
gene cassette with cyanamide hydratase (cah) marker gene for antifungal activity assays in transgenic plants, regeneration of transgenic plants achieved in selective media amended with 5 mM cyanamide
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). DDI2_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
225
0
25152
Swiss-Prot
other Location (Reliability: 5)
DDI3_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
225
0
25152
Swiss-Prot
other Location (Reliability: 5)
CYAH_ALBVE
244
0
26966
Swiss-Prot
other Location (Reliability: 2)
Q4WGU6_ASPFU
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
259
0
28666
TrEMBL
other Location (Reliability: 1)
A2QXJ8_ASPNC
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
259
0
28800
TrEMBL
other Location (Reliability: 2)
A2R1K9_ASPNC
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
244
0
27613
TrEMBL
other Location (Reliability: 1)
I7G8S2_MYCS2
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
199
0
21791
TrEMBL
-
B9WAJ2_CANDC
Candida dubliniensis (strain CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 / NRRL Y-17841)
229
0
26111
TrEMBL
other Location (Reliability: 2)
I7GBG9_MYCS2
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
218
0
24309
TrEMBL
-
I7G498_MYCS2
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
199
0
21842
TrEMBL
-
A7A1Y4_YEAS7
Saccharomyces cerevisiae (strain YJM789)
226
0
25268
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, strains BL 21 (DE3) and JM109, pCAMBIA 1300 vector used for construction of gene cassette for genetic transformation in Nicotiana tabacum, cyanamide hydratase (cah) used as a marker gene
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the expression of the Cah enzyme in soybean leaves does not greatly affect the concentrations of any of the metabolites measured in unsprayed 1C and Jack plants grown under normal conditions or when placed in the dark for 24 h
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transgenic soybean plants can be produced that express the fungal Cah gene and thus acquire resistance to toxic cyanamide. These plants are phenotypically normal and fertile indicating that expression of the Cah protein has no effect on soybean plant metabolism
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wild-type cah and its mutants are cloned and overexpressed in pQE-60 Escherichia coli expression system. Western blot analysis confirms the production of 27700 Da cah enzyme by all the recombinants. Agrobacterium-mediated transformation is performed in Nicotiana tabaccum cv. Samsun plants by employing the leaf-disc method. The integration and expression of cah gene in transgenic plants are confirmed by polymerase chain reaction, Southern and Western blot analyses
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
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due to its innate ability to convert cyanamide to urea and the broad-spectrum antimicrobial activity of cyanamide, the cah gene can be used to facilitate plant growth promotion and biocontrol of phytopathogens
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Stransky, H.; Amberger, A.
Isolierung und Eigenschaften einer Cyanamid-hydratase (E.C.-Gruppe 4.2.1.) aus Myrothecium verrucaria
Z. Pflanzenphysiol.
70
74-87
1973
Albifimbria verrucaria
-
brenda
Kufrevioglu, O.I.; Hartmann, G.
Partial purification of cyanamide hydratase from Myrothecium verrucaria
Doga Tr. J. Chem.
14
332-338
1990
Albifimbria verrucaria
-
brenda
Zhang, X.H.; Zhong, W.Q.; Widholm, J.M.
Expression of a fungal cyanamide hydratase in transgenic soybean detoxifies cyanamide in tissue culture and in planta to provide cyanamide resistance
J. Plant Physiol.
162
1064-1073
2005
Albifimbria verrucaria
brenda
Kirubakaran, S.I.; Sakthivel, N.
Site-directed mutagenesis, heterologous expression of cyanamide hydratase gene and antimicrobial activity of cyanamide
Curr. Microbiol.
56
42-47
2008
Albifimbria verrucaria
brenda
Ulanov, A.; Widholm, J.M.
Effect of the expression of cyanamide hydratase on metabolites in cyanamide-treated soybean plants kept in the light or dark
J. Exp. Bot.
58
4319-4332
2007
Albifimbria verrucaria
brenda
Kirubakaran, S.I.; Begum, S.M.; Ulaganathan, K.; Sakthivel, N.
Characterization of a new antifungal lipid transfer protein from wheat
Plant Physiol. Biochem.
46
918-927
2008
Nicotiana tabacum
brenda
van Driel, H.; Keppel, M.; van den Berg, E.; Schoo, W.; van der Lans, M.; Notkamp, M.; Jansen, E.; Roebroeck, E.; Ohl, S.; Stuiver-Hoekstra, S.
Cyanamide hydratase as selectable marker in potato
Plant Cell
118
125-135
2014
Solanum tuberosum
-
brenda
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