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Information on EC 4.2.1.45 - CDP-glucose 4,6-dehydratase
for references in articles please use BRENDA:EC4.2.1.45
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IUBMB Comments
Requires bound NAD+.
The enzyme appears in viruses and cellular organisms
- 4.2.1.45
- yersinia
-
nad+-dependent
- nad+
- pseudotuberculosis
-
3,6-dideoxyhexose
-
4\'-hydroxyl
showSynonyms
cdp-d-glucose 4,6-dehydratase, cdp-glucose 4,6-dehydratase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CDP-D-glucose 4,6-dehydratase
CDP-D-glucose-4,6-dehydratase
-
-
-
-
cytidine diphosphoglucose oxidoreductase
-
-
-
-
dehydratase, cytidine diphosphoglucose
-
-
-
-
CDP-D-glucose 4,6-dehydratase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CDP-glucose = CDP-4-dehydro-6-deoxy-D-glucose + H2O
stereochemistry, intramolecular oxidation-reduction that involves an internal hydrogen transfer from C-4 of the substrate to C-6 of the resulting product
-
CDP-glucose = CDP-4-dehydro-6-deoxy-D-glucose + H2O
displacement of the hydroxyl-group at C-6 is stereospecific and occurs with inversion
-
CDP-glucose = CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
elimination
-
-
-
-
intramolecular oxido-reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis, CDP-6-deoxy-D-gulose biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
CDP-glucose 4,6-hydro-lyase (CDP-4-dehydro-6-deoxy-D-glucose-forming)
Requires bound NAD+.
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CAS REGISTRY NUMBER
COMMENTARY
37259-55-5
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CDP-glucose
?
-
Substrates: biosynthetic pathway of 3,6-dideoxysugars
Products: -
Products: -
?
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
?
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
?
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
?
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
ir
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
?
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
ir
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
?
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: CDP-6-deoxy-D-glycero-L-threo-4-hexulose
Products: CDP-6-deoxy-D-glycero-L-threo-4-hexulose
?
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
?
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
?
dCDP-D-glucose
dCDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
ir
dCDP-D-glucose
dCDP-4-dehydro-6-deoxy-D-glucose + H2O
-
Substrates: -
Products: -
Products: -
ir
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CDP-glucose
?
-
Substrates: biosynthetic pathway of 3,6-dideoxysugars
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
catalytic prosthetic group, NAD+ serves as a hydride carrier receiving the 4H of the substrate in the oxidative half-reaction and passing the reducing equivalents to C-6 in the reductive half-reaction, the substrate is bound to the si-face of the nicotinamide ring
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line Links Show AA Sequence and Transmembrane Helices (196 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
tetramer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallization of selenomethionine-labeled CDP-D-glucose 4,5-dehydratase and of selenomethionine-labeled CDP-D-glucose 4,5-dehydratase complexed with the substrate analog CDP-D-xylose, hanging-drop vapor diffusion, batch method at room temperature
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable to freezing in all stages of purification
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Glaser, L.; Zarkowsky, H.
Dehydration in nucleotide-linked deoxysugar synthesis
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
5
465-480
1971
Salmonella enterica subsp. enterica serovar Typhimurium
-
brenda
Bevill, R.D.
Cytidin-5'-diphospho-glucose
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
2
2260-2263
1974
Salmonella enterica subsp. enterica serovar Typhimurium
-
brenda
Hey, A.E.; Elbein, A.D.
Biosynthesis of tyvelose. The purification and properties of cytidine diphosphate D-glucose oxidoreductase
J. Biol. Chem.
241
5473-5478
1966
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Matsuhashi, S.; Matsuhashi, M.; Brown, J.G.; Strominger, J.L.
Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. 3. Cytidine diphosphate D-glucose oxidoreductase
J. Biol. Chem.
241
4283-4287
1966
Yersinia pseudotuberculosis
brenda
He, X.; Thorson, J.S.; Liu, H.
Probing the coenzyme and substrate binding events of CDP-D-glucose 4,6-dehydratase: mechanistic implications
Biochemistry
35
4721-4731
1996
Yersinia pseudotuberculosis
brenda
Gavini, N.; Hausman, B.S.; Pulakat, L.; Schreiner, R.P.; Williamson, J.A.
Identification and mutational analysis of rfbG, the gene encoding CDP-D-glucose-4,6-dehydratase, isolated from free living soil bacterium Azotobacter vinelandii
Biochem. Biophys. Res. Commun.
240
153-161
1997
Azotobacter vinelandii
brenda
Hallis, R.M.; Liu, H.
Stereospecificity of hydride transfer for the catalytically recycled NAD+ in CDP-D-glucose 4,6-dehdratase
Tetrahedron
54
15975-15982
1998
Yersinia pseudotuberculosis
-
brenda
Chang, C.W.T.; Chen, X.H.; Liu, H.
CDP-6-deoxy-6,6-difluoro-D-glucose: a mechanism-based inhibitor for CDP-D-glucose 4,6-dehydratase
J. Am. Chem. Soc.
120
9698-9699
1998
Yersinia pseudotuberculosis
-
brenda
Russell, R.N.; Liu, H.w.
Stereochemical and mechanistic studies of CDP-D-glucose oxidoreductase isolated from Yersinia pseudotuberculosis
J. Am. Chem. Soc.
113
7777-7778
1991
Yersinia pseudotuberculosis
-
brenda
Yu, Y.; Russell, R.N.; Thorson, J.S.; Liu, L.i.; Liu, H.w.
Mechanistic studies of the biosynthesis of 3,6-dideoxyhexoses in Yersinia pseudotuberculosis. Purification and stereochemical analysis of CDP-D-glucose oxidoreductase
J. Biol. Chem.
267
5858-5875
1992
Yersinia pseudotuberculosis
-
brenda
Gonzalez-Porque, P.; Strominger, J.L.
Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. VI. Purification to homogeneity and some properties of cytidine diphosphate-D-glucose oxidoreductase, enzyme E1 and enzyme E3
J. Biol. Chem.
247
6748-6756
1972
Yersinia pseudotuberculosis
brenda
Vogan, E.M.; Bellamacina, C.R.; He, X.; Foxman, B.M.; Ringe, D.; Liu, H.W.; Petsko, G.A.
Purification, crystallization and molecular symmetry of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosis
Acta Crystallogr. Sect. D
58
370-373
2002
Yersinia pseudotuberculosis
brenda
Vogan, E.M.; Bellamacina, C.; He, X.; Liu, H.W.; Ringe, D.; Petsko, G.A.
Crystal structure at 1.8 A resolution of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosis
Biochemistry
43
3057-3067
2004
Yersinia pseudotuberculosis
brenda
Koropatkin, N.M.; Holden, H.M.
Structure of CDP-D-glucose 4,6-dehydratase from Salmonella typhi complexed with CDP-D-xylose
Acta Crystallogr. Sect. D
61
365-373
2005
Salmonella enterica subsp. enterica serovar Typhi
brenda
EXTERNAL LINKS (specific for EC-Number 4.2.1.45)
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