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IUBMB CommentsThe enzyme, characterized from several bacterial species, is involved in an L-lyxonate degradation pathway.
The expected taxonomic range for this enzyme is: Pseudomonas aeruginosa
Synonyms
l-lyxonate dehydratase,
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lyxD
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lyxD
Roseibium aggregatum
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L-lyxonate = 2-dehydro-3-deoxy-L-arabinonate + H2O
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L-lyxonate hydro-lyase
The enzyme, characterized from several bacterial species, is involved in an L-lyxonate degradation pathway.
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D-gulonate
L-lyxonate + H2O
L-lyxonate
2-dehydro-3-deoxy-L-arabinonate + H2O
additional information
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D-gulonate
L-lyxonate + H2O
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D-gulonate
L-lyxonate + H2O
Roseibium aggregatum
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L-lyxonate
2-dehydro-3-deoxy-L-arabinonate + H2O
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L-lyxonate
2-dehydro-3-deoxy-L-arabinonate + H2O
Roseibium aggregatum
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additional information
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no activity with L-mannonate
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additional information
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Roseibium aggregatum
no activity with L-mannonate
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L-lyxonate
2-dehydro-3-deoxy-L-arabinonate + H2O
L-lyxonate
2-dehydro-3-deoxy-L-arabinonate + H2O
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L-lyxonate
2-dehydro-3-deoxy-L-arabinonate + H2O
Roseibium aggregatum
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Mg2+
contains Mg2+, 5 mM used in assay conditions
Mg2+
Roseibium aggregatum
contains Mg2+, 5 mM used in assay conditions
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1
D-gulonate
Roseibium aggregatum
wild type enzyme, at pH 7.5 and 30°C
0.2
L-lyxonate
Roseibium aggregatum
wild type enzyme, at pH 7.5 and 30°C
0.2
L-lyxonate
Roseibium aggregatum
mutant enzyme Y216F, at pH 7.5 and 30°C
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3
D-gulonate
Roseibium aggregatum
wild type enzyme, at pH 7.5 and 30°C
2
L-lyxonate
Roseibium aggregatum
wild type enzyme, at pH 7.5 and 30°C
2
L-lyxonate
Roseibium aggregatum
mutant enzyme Y216F, at pH 7.5 and 30°C
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3
D-gulonate
Roseibium aggregatum
wild type enzyme, at pH 7.5 and 30°C
10
L-lyxonate
Roseibium aggregatum
wild type enzyme, at pH 7.5 and 30°C
10
L-lyxonate
Roseibium aggregatum
mutant enzyme Y216F, at pH 7.5 and 30°C
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UniProt
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Roseibium aggregatum
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UniProt
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A0NP48_ROSAI
Roseibium aggregatum (strain ATCC 25650 / DSM 13394 / JCM 20685 / NBRC 16684 / NCIMB 2208 / IAM 12614 / B1)
390
0
43690
TrEMBL
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Q9I1Q2_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
391
0
44202
TrEMBL
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in complex with Mg2+, sitting drop vapor diffusion method, using 0.5 M magnesium formate and 0.1 M Bis-Tris at pH 6.5
Roseibium aggregatum
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H316Q
Roseibium aggregatum
inactive
R179K
Roseibium aggregatum
insoluble protein
R179Q
Roseibium aggregatum
inactive
Y216F
Roseibium aggregatum
the mutation does not affect the activity of the enzyme
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Ni-NTA column chromatography and HiLoad S200 gel filtration
Roseibium aggregatum
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expressed in Escherichia coli BL21(DE3) cells
Roseibium aggregatum
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Ghasempur, S.; Eswaramoorthy, S.; Hillerich, B.S.; Seidel, R.D.; Swaminathan, S.; Almo, S.C.; Gerlt, J.A.
Discovery of a novel L-lyxonate degradation pathway in Pseudomonas aeruginosa PAO1
Biochemistry
53
3357-3366
2014
Roseibium aggregatum (A0NP48), Pseudomonas aeruginosa (Q9I1Q2)
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4.2.1.176 L-lyxonate dehydratase
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