solubilization of the cyclase in 8 mg/ml digitonin inactivates the enzyme, its activity can be recovered by complementation of the assay buffer with octylthioglucoside above its critical micellar concentration
solubilization of the cyclase in 30 mg/ml Tween 80 inactivates the enzyme, its activity can be recovered by complementation of the assay buffer with octylthioglucoside above its critical micellar concentration
genes encoding the tetrahymanol synthesizing enzyme squalene-tetrahymanol cyclase are found from several phylogenetically diverged eukaryotes that live in oxygen-poor environments and appear to have been laterally transferred among such eukaryotes, overview
genes encoding the tetrahymanol synthesizing enzyme squalene-tetrahymanol cyclase are found from several phylogenetically diverged eukaryotes that live in oxygen-poor environments and appear to have been laterally transferred among such eukaryotes, overview
genes encoding the tetrahymanol synthesizing enzyme squalene-tetrahymanol cyclase are found from several phylogenetically diverged eukaryotes that live in oxygen-poor environments and appear to have been laterally transferred among such eukaryotes, overview
genes encoding the tetrahymanol synthesizing enzyme squalene-tetrahymanol cyclase are found from several phylogenetically diverged eukaryotes that live in oxygen-poor environments and appear to have been laterally transferred among such eukaryotes, overview
genes encoding the tetrahymanol synthesizing enzyme squalene-tetrahymanol cyclase are found from several phylogenetically diverged eukaryotes that live in oxygen-poor environments and appear to have been laterally transferred among such eukaryotes, overview
genes encoding the tetrahymanol synthesizing enzyme squalene-tetrahymanol cyclase are found from several phylogenetically diverged eukaryotes that live in oxygen-poor environments and appear to have been laterally transferred among such eukaryotes, overview
genes encoding the tetrahymanol synthesizing enzyme squalene-tetrahymanol cyclase are found from several phylogenetically diverged eukaryotes that live in oxygen-poor environments and appear to have been laterally transferred among such eukaryotes, overview
phylogenetic evidence reveals that the last common ancestor of diplomonads was dependent on a host and that Trepomonas has adapted secondarily to a free-living lifestyle. Free-living taxa are nested within parasites reveals more examples of secondarily free-living eukaryotes, transcriptome study of Trepomonas sp. PC1, a diplomonad isolated from marine sediment. Phylogenetic analyses show that most of the differences in metabolic capacity between free-living Trepomonas and the parasitic diplomonads are due to recent acquisitions of bacterial genes via gene transfer. The transcriptome includes a gene encoding squalene-tetrahymanol cyclase. This enzyme synthesizes the sterol substitute tetrahymanol in the absence of oxygen, potentially allowing Trepomonas to thrive under anaerobic conditions as a free-living bacterivore, without depending on sterols from other eukaryotes
the eukaryotic squalene-tetrahymanol cyclase is homologous to the bacterial tetrahymanol synthase (Ths), that is not encoded in any eukaryotic genomes. Bacterial tetrahymanol synthase (Ths) is found in a variety of bacterial genomes, including aerobic methanotrophs, nitrite-oxidizers, and sulfate-reducers, and in a subset of aquatic and terrestrial metagenomes
the eukaryotic squalene-tetrahymanol cyclase is homologous to the bacterial tetrahymanol synthase (Ths), that is not encoded in any eukaryotic genomes. Bacterial tetrahymanol synthase (Ths) is found in a variety of bacterial genomes, including aerobic methanotrophs, nitrite-oxidizers, and sulfate-reducers, and in a subset of aquatic and terrestrial metagenomes
eukaryotic squalene-tetrahymanol cyclase catalyzes the cyclization of squalene directly to tetrahymanol, a polycyclic triterpenoid lipid, the enzyme catalyzes the cyclization of squalene directly to tetrahymanol
the enzyme synthesizes the sterol substitute tetrahymanol in the absence of oxygen, potentially allowing Trepomonas to thrive under anaerobic conditions as a free-living bacterivore, without depending on sterols from other eukaryotes. Several microbial eukaryotes that live under anoxic conditions employ the sterol substitute tetrahymanol, which can be synthesized without molecular oxygen. The Trepomonas transcriptome includes a homologue of STC, the enzyme required to synthesize tetrahymanol
eukaryotic squalene-tetrahymanol cyclase catalyzes the cyclization of squalene directly to tetrahymanol, a polycyclic triterpenoid lipid, the enzyme catalyzes the cyclization of squalene directly to tetrahymanol
tetrahymanol, a triterpenoid that does not require molecular oxygen for its biosynthesis, likely functions as a surrogate of sterol in eukaryotes inhabiting oxygen-poor environments
tetrahymanol, a triterpenoid that does not require molecular oxygen for its biosynthesis, likely functions as a surrogate of sterol in eukaryotes inhabiting oxygen-poor environments
tetrahymanol, a triterpenoid that does not require molecular oxygen for its biosynthesis, likely functions as a surrogate of sterol in eukaryotes inhabiting oxygen-poor environments
tetrahymanol, a triterpenoid that does not require molecular oxygen for its biosynthesis, likely functions as a surrogate of sterol in eukaryotes inhabiting oxygen-poor environments
tetrahymanol, a triterpenoid that does not require molecular oxygen for its biosynthesis, likely functions as a surrogate of sterol in eukaryotes inhabiting oxygen-poor environments
tetrahymanol, a triterpenoid that does not require molecular oxygen for its biosynthesis, likely functions as a surrogate of sterol in eukaryotes inhabiting oxygen-poor environments
tetrahymanol, a triterpenoid that does not require molecular oxygen for its biosynthesis, likely functions as a surrogate of sterol in eukaryotes inhabiting oxygen-poor environments
tetrahymanol is a polycyclic triterpenoid lipid in the Tetrahymena ciliates. The diagenetic product, gammacerane, is often used as a biomarker for water column stratification in ancient ecosystems
tetrahymanol is a polycyclic triterpenoid lipid in the Tetrahymena ciliates. The diagenetic product, gammacerane, is often used as a biomarker for water column stratification in ancient ecosystems
tetrahymanol is a polycyclic triterpenoid lipid in the Tetrahymena ciliates. The diagenetic product, gammacerane, is often used as a biomarker for water column stratification in ancient ecosystems
no activity in Rhodopseudomonas palustris, Tetrahymena thermophila, no activity in Methylococcus capsulatus, no activity in Alicyclobacillus acidocaldarius, no activity in Bradyrhizobium japonicum
Lateral transfer of tetrahymanol-synthesizing genes has allowed multiple diverse eukaryote lineages to independently adapt to environments without oxygen