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Information on EC 4.2.1.106 - bile-acid 7alpha-dehydratase for references in articles please use BRENDA:EC4.2.1.106
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EC Tree
IUBMB Comments This enzyme, characterized from the gut bacterium Clostridium scindens (previously known as Eubacterium sp. strain VPI 12708), participates in the 7-dehydroxylation process associated with bile acid degradation.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
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baiE
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7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA = 12alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA + H2O
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoyl-CoA hydro-lyase (12alpha-hydroxy-3-oxochola-4,6-dienoyl-CoA-forming)
This enzyme, characterized from the gut bacterium Clostridium scindens (previously known as Eubacterium sp. strain VPI 12708), participates in the 7-dehydroxylation process associated with bile acid degradation.
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
7alpha-hydroxy-3-oxochol-4-enoate
3-oxocholate-4,6-dienoate
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-enoate
3-oxocholate-4,6-dienoate
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84% of activity with 7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
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7alpha-hydroxy-3-oxochol-4-enoate
3-oxocholate-4,6-dienoate
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84% of activity with 7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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0.16
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
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pH 7.5
9.9 - 25
7alpha-hydroxy-3-oxochol-4-en-24-oate
1.2 - 208
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
9.9
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl pH 7.3
11
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
21
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
25
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
1.2
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
4
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl pH 7.3
11.4
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
208
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
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4.4 - 24
7alpha-hydroxy-3-oxochol-4-en-24-oate
12.6 - 98
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
4.4
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
18
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
18.6
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
24
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl pH 7.3
12.6
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
15
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
22.2
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
98
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl pH 7.3
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0.2 - 2.4
7alpha-hydroxy-3-oxochol-4-en-24-oate
0.1 - 24.5
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
0.2
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
0.9
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
1.6
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
2.4
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl pH 7.3
0.1
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
1.1
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
12.5
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
24.5
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl pH 7.3
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SwissProt
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SwissProt
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type strain TO-931
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Clostridium scindens (strain ATCC 35704 / DSM 5676 / VPI 13733 / 19)
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19500
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2 * 19500, deduced from nucleotide sequence
23000
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2 * 23000, SDS-PAGE
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dimer
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2 * 19500, deduced from nucleotide sequence; 2 * 23000, SDS-PAGE
dimer
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2 * 19500, deduced from nucleotide sequence; 2 * 23000, SDS-PAGE
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Y126F
strongly reduced activity compared to wild type
Y54F
reduced activity compared to wild type
D106N
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inactive mutant
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Y54F
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reduced activity compared to wild type
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DE-52, Phenyl-Sepharose, HPLC-DEAE, gel filtration
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cloning of bai operon containing BaiE gene coding for bile acid 7alpha-dehydratase
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expression in Escherichia coli
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Wells, J.E.; Hylemon, P.B.
Identification and characterization of a bile acid 7alpha-dehydroxylation operon in Clostridium sp. strain TO-931, a highly active 7alpha-dehydroxylating strain isolated from human feces
Appl. Environ. Microbiol.
66
1107-1113
2000
Clostridium sp., Clostridium sp. TO-931
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Kitahara, M.; Takamine, F.; Imamura, T.; Benno, Y.
Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7alpha-dehydroxylating activity
Int. J. Syst. Evol. Microbiol.
51
39-44
2001
Clostridium hiranonis
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Coleman, J.P.; White, W.B.; Egestad, B.; Sjoevall, J.; Hylemon, P.B.
Biosynthesis of a novel bile acid nucleotide and mechanism of 7alpha-dehydroxylation by an intestinal Eubacterium species
J. Biol. Chem.
262
4701-4707
1987
Eubacterium sp., Eubacterium sp. VPI 12708
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Dawson, J.A.; Mallonee, D.H.; Bjorkhem, I.; Hylemon, P.B.
Expression and characterization of a C24 bile acid 7alpha-dehydratase from Eubacterium sp. strain VPI 12708 in Escherichia coli
J. Lipid Res.
37
1258-1267
1996
Eubacterium sp., Eubacterium sp. VPI 12708
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Bhowmik, S.; Chiu, H.P.; Jones, D.H.; Chiu, H.J.; Miller, M.D.; Xu, Q.; Farr, C.L.; Ridlon, J.M.; Wells, J.E.; Elsliger, M.A.; Wilson, I.A.; Hylemon, P.B.; Lesley, S.A.
Structure and functional characterization of a bile acid 7alpha dehydratase BaiE in secondary bile acid synthesis
Proteins
84
316-331
2016
Clostridium hiranonis (Q9RB47), Clostridium hiranonis DSM 13275 (Q9RB47), Clostridium hylemonae (B4YSU1), Clostridium hylemonae DSM 15053 (B4YSU1), Clostridium scindens (B0NI18), Clostridium scindens (P19412), Clostridium scindens ATCC 35704 (B0NI18), Clostridium scindens VPI 12708 (P19412)
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Enzyme Nomenclature
4.2.1.106 bile-acid 7alpha-dehydratase
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