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EC Tree
IUBMB Comments This enzyme, characterized from the gut bacterium Clostridium scindens (previously known as Eubacterium sp. strain VPI 12708), participates in the 7-dehydroxylation process associated with bile acid degradation.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
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baiE
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7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA = 12alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA + H2O
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoyl-CoA hydro-lyase (12alpha-hydroxy-3-oxochola-4,6-dienoyl-CoA-forming)
This enzyme, characterized from the gut bacterium Clostridium scindens (previously known as Eubacterium sp. strain VPI 12708), participates in the 7-dehydroxylation process associated with bile acid degradation.
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
7alpha-hydroxy-3-oxochol-4-enoate
3-oxocholate-4,6-dienoate
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-enoate
3-oxocholate-4,6-dienoate
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84% of activity with 7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
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7alpha-hydroxy-3-oxochol-4-enoate
3-oxocholate-4,6-dienoate
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84% of activity with 7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
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0.16
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
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pH 7.5
9.9 - 25
7alpha-hydroxy-3-oxochol-4-en-24-oate
1.2 - 208
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
9.9
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl pH 7.3
11
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
21
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
25
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
1.2
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
4
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl pH 7.3
11.4
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
208
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
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4.4 - 24
7alpha-hydroxy-3-oxochol-4-en-24-oate
12.6 - 98
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
4.4
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
18
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
18.6
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
24
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl pH 7.3
12.6
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
15
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
22.2
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
98
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl pH 7.3
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0.2 - 2.4
7alpha-hydroxy-3-oxochol-4-en-24-oate
0.1 - 24.5
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
0.2
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
0.9
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
1.6
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl, pH 7.3
2.4
7alpha-hydroxy-3-oxochol-4-en-24-oate
25°C in 20 mM Tris-HCl pH 7.3
0.1
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
1.1
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
12.5
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl, pH 7.3
24.5
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
25°C in 20 mM Tris-HCl pH 7.3
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SwissProt
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SwissProt
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SwissProt
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SwissProt
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SwissProt
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SwissProt
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SwissProt
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type strain TO-931
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brenda
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Clostridium scindens (strain ATCC 35704 / DSM 5676 / VPI 13733 / 19)
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19500
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2 * 19500, deduced from nucleotide sequence
23000
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2 * 23000, SDS-PAGE
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dimer
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2 * 23000, SDS-PAGE
dimer
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2 * 19500, deduced from nucleotide sequence
dimer
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2 * 23000, SDS-PAGE
dimer
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2 * 19500, deduced from nucleotide sequence
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-
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Y126F
strongly reduced activity compared to wild type
Y54F
reduced activity compared to wild type
Y54F
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reduced activity compared to wild type
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DE-52, Phenyl-Sepharose, HPLC-DEAE, gel filtration
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cloning of bai operon containing BaiE gene coding for bile acid 7alpha-dehydratase
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expression in Escherichia coli
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Wells, J.E.; Hylemon, P.B.
Identification and characterization of a bile acid 7alpha-dehydroxylation operon in Clostridium sp. strain TO-931, a highly active 7alpha-dehydroxylating strain isolated from human feces
Appl. Environ. Microbiol.
66
1107-1113
2000
Clostridium sp., Clostridium sp. TO-931
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Kitahara, M.; Takamine, F.; Imamura, T.; Benno, Y.
Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7alpha-dehydroxylating activity
Int. J. Syst. Evol. Microbiol.
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39-44
2001
[Clostridium] hiranonis
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Coleman, J.P.; White, W.B.; Egestad, B.; Sjoevall, J.; Hylemon, P.B.
Biosynthesis of a novel bile acid nucleotide and mechanism of 7alpha-dehydroxylation by an intestinal Eubacterium species
J. Biol. Chem.
262
4701-4707
1987
Eubacterium sp., Eubacterium sp. VPI 12708
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Dawson, J.A.; Mallonee, D.H.; Bjorkhem, I.; Hylemon, P.B.
Expression and characterization of a C24 bile acid 7alpha-dehydratase from Eubacterium sp. strain VPI 12708 in Escherichia coli
J. Lipid Res.
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1258-1267
1996
Eubacterium sp., Eubacterium sp. VPI 12708
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Bhowmik, S.; Chiu, H.P.; Jones, D.H.; Chiu, H.J.; Miller, M.D.; Xu, Q.; Farr, C.L.; Ridlon, J.M.; Wells, J.E.; Elsliger, M.A.; Wilson, I.A.; Hylemon, P.B.; Lesley, S.A.
Structure and functional characterization of a bile acid 7alpha dehydratase BaiE in secondary bile acid synthesis
Proteins
84
316-331
2016
[Clostridium] scindens (B0NI18), [Clostridium] scindens (P19412), [Clostridium] hylemonae (B4YSU1), [Clostridium] hiranonis (Q9RB47), [Clostridium] scindens ATCC 35704 (B0NI18), [Clostridium] hiranonis DSM 13275 (Q9RB47), [Clostridium] hylemonae DSM 15053 (B4YSU1), [Clostridium] scindens VPI 12708 (P19412)
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Enzyme Nomenclature
4.2.1.106 bile-acid 7alpha-dehydratase
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