Information on EC 3.6.1.8 - ATP diphosphatase

for references in articles please use BRENDA:EC3.6.1.8
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Eukaryota, Archaea

top print hide
EC NUMBER
COMMENTARY hide
3.6.1.8
-
top print hide
RECOMMENDED NAME
GeneOntology No.
ATP diphosphatase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O = AMP + diphosphate
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
-
-
Pyrimidine metabolism
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
ATP diphosphohydrolase (diphosphate-forming)
Also acts on ITP, GTP, CTP and UTP.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
37289-25-1
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
snake
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
acellular slime mold
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
AMP + diphosphate
show the reaction diagram
beta, gamma-imido ATP + H2O
AMP + imido diphosphate
show the reaction diagram
-
-
-
?
beta,gamma-methylene ATP + H2O
AMP + methylene diphosphate
show the reaction diagram
CTP + H2O
CMP + diphosphate
show the reaction diagram
-
-
-
?
dATP + H2O
dAMP + diphosphate
show the reaction diagram
-
-
-
?
GTP + H2O
GMP + diphosphate
show the reaction diagram
ITP + H2O
IMP + diphosphate
show the reaction diagram
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
AMP + diphosphate
show the reaction diagram
CTP + H2O
CMP + diphosphate
show the reaction diagram
-
-
-
?
GTP + H2O
GMP + diphosphate
show the reaction diagram
-
-
-
?
ITP + H2O
IMP + diphosphate
show the reaction diagram
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
no effect
Cu2+
-
no effect
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15-desoxybludein
-
0.065 mg/ml, 11% inhibition
-
AMP
-
slight inhibition
Caffeine
-
25% inhibition at 3 mM
cAMP
-
20% inhibition at 3 mM
eremantolide C
-
0.065 mg/ml, 17% inhibition
glaucolide A
-
0.065 mg/ml, 11% inhibition
glaucolide B
-
0.065 mg/ml, 16% inhibition
GMP
-
competitive inhibition with Ki: 0.001 mM
goyazensolide
-
0.065 mg/ml, 10% inhibition
isogoyazensolide
-
0.065 mg/ml, 11% inhibition
-
licnofolide
-
0.065 mg/ml, 22% inhibition
phosphatidylserine
-
-
thapsigargicin
-
0.065 mg/ml, 57% inhibition
thapsigargin
-
inhibits ATP hydrolysis, 70% inhibition at 0.065 mg/ml, mixed type inhibition, little or not affected by changes in free Ca2+ or Mg2+ concentrations, hydrolysis of ADP is not inhibited
UDPGlc
-
very weak
additional information
-
no inhibition by 0.065 mg/ml 15-desoxygoyazensolide
-
top print hide
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-isobutyl-1-methylxanthine
-
activation in preincubation
cAMP
-
weak activation
glucose
-
activation in preincubation
NaF
-
20 mM
NaN3
-
10 mM
oleic acid
-
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00032 - 2
ATP
0.0022
GTP
-
with 0.1 mM free Ca2+, pH 8
top print hide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
GMP
-
competitive inhibition
0.02
thapsigargin
-
37°C, pH 7.4, inhibition of ATP hydrolysis
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.03 - 0.04
-
with ATP as substrate
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
broad optimum range
8.4 - 9
-
-
9.9
-
particular enzymes
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
20% activity at pH 6, 50% activity at pH 10
7.4 - 8.8
-
much higher activity at pH 8.8
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
additional information
-
18 tissues examined
Manually annotated by BRENDA team
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
two different localisations found
-
Manually annotated by BRENDA team
top print hide
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
2 * 28000, SDS-PAGE
49000
gel filtration
56000
-
SDS-PAGE
61000
-
SDS-PAGE, soluble enzyme
127000
-
SDS-PAGE, sedimentable enzyme
240000
-
gel filtration
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 28000, SDS-PAGE
tetramer
-
4 * 56000, SDS-PAGE and gel filtration
top print hide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
30 min at 60°C causes no loss of activity
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
top
Show AA Sequence (1138 entries)
Please use the Sequence Search for a specific query.
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 3.6.1.8)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)